Cell-based reporters reveal in vivo dynamics of dopamine and norepinephrine release in murine cortex
Open accessAbstract
The neuronal coding of stimulus-to-action sequences is believed to involve the release of dopamine (DA) and norepinephrine (NE). The electrochemical similarity of these monoamines, however, confounds real-time measurements of their release. Here we report cell-based neurotransmitter fluorescent engineered reporters (CNiFERs) that use the specificity of G protein-coupled receptors (GPCRs) to discriminate nanomolar concentrations of DA and NE. CNiFERs were implanted into the frontal cortex of mice to measure the timing of neurotransmitter release during classical conditioning with the use of two-photon microscopy. The onset of DA release correlated with that of licking and shifted from the time of the reward toward that of the cue upon conditioning. In contrast, concurrent release of NE did not correlate with licking or the cue. This generation of CNiFERs provides unique tools to assess the release of monoamines. The molecular design of these CNiFERs may be generalized to realize CNiFERs for any molecule that activates a GPCR.
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Catecholamine secretion can be monitored in a real-time manner using specific probes.
“Catecholamine secretion can be monitored in a real-time manner using specific probes (Muller et al., 2014; Wang et al., 2018).”
From Intestinal microbiota modulates adrenomedullary response through Nod1 sensing in chromaffin cells (2021) · cites this paper as Muller et al., 2014
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- Authors
- Muller, A., Joseph, V., Slesinger, P. A., & Kleinfeld, D.
- Affiliations
- University of California San Diego (US)
- Corresponding author
- Slesinger, Paul A
- Kleinfeld, David
- Venue
- Nature Methods
- Published
- 2014-10-26
- Volume
- 11
- Issue
- 12
- Pages
- 1245-1252
- ISSN
- 1548-7091 (ISSN-L)
- 1548-7105
- PMC
- PMC4245316
- OpenAlex
- W2123300469
- Cited by
- 139
- References
- 53
- Citations by year
- 2026: 4 · 2025: 10 · 2024: 12 · 2023: 14 · 2022: 13 · 2021: 17 · 2020: 17 · 2019: 12 · 2018: 18 · 2017: 6 · 2016: 11 · 2015: 4 · 2014: 1
- FWCI
- 4.36
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature Methods (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institutes of Health (DP1OD006831)
- University of California, San Diego
- National Institute on Drug Abuse (DA007315, R01DA029706, DA029706)
- National Institute of Neurological Disorders and Stroke (DP1NS082097)
- National Institute of Biomedical Imaging and Bioengineering (R01EB003832, EB/003832)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Advanced Fluorescence Microscopy Techniques
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurotransmitter Receptor Influence on Behavior
- Keywords
- dopamine release, noradrenaline release, G protein-coupled receptors, frontal cortex, classical conditioning, monoamine release, two-photon microscopy, in vivo imaging
- MeSH terms
- Animals, Behavior, Animal, Cerebral Cortex, Dopamine, Fluorescent Dyes, Learning, Synaptic Transmission, Norepinephrine, Fluorescence Resonance Energy Transfer, Receptors, G-Protein-Coupled, Mice
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