Differential co-localisation of the P2X7 receptor subunit with vesicular glutamate transporters VGLUT1 and VGLUT2 in rat CNS
Abstract
Presynaptic P2X(7) receptors are thought to play a role in the modulation of transmitter release and have been localised to terminals with the location and morphology typical of excitatory boutons. To test the hypothesis that this receptor is preferentially associated with excitatory terminals we combined immunohistochemistry for the P2X(7) receptor subunit (P2X(7)R) with that for two vesicular glutamate transporters (VGLUT1 and VGLUT2) in the rat CNS. This confirmed that P2X(7)R immunoreactivity (IR) is present in glutamatergic terminals; however, whether it was co-localised with VGLUT1-IR or VGLUT2-IR depended on the CNS region examined. In the spinal cord, P2X(7)R-IR co-localised with VGLUT2-IR. In the brainstem, co-localisation of P2X(7)R-IR with VGLUT2-IR was widespread, but co-localisation with VGLUT1-IR was seen only in the external cuneate nucleus and spinocerebellar tract region of the ventral medulla. In the cerebellum, P2X(7)R-IR co-localised with both VGLUT1 and VGLUT2-IR in the granular layer. In the hippocampus it was co-localised only with VGLUT1-IR, including in the polymorphic layer of the dentate gyrus and the substantia radiatum of the CA3 region. In other forebrain areas, P2X(7)R-IR co-localised with VGLUT1-IR throughout the amygdala, caudate putamen, striatum, reticular thalamic nucleus and cortex and with VGLUT2-IR in the dorsal lateral geniculate nucleus, amygdala and hypothalamus. Dual labelling studies performed using markers for cholinergic, monoaminergic, GABAergic and glycinergic terminals indicated that in certain brainstem and spinal cord nuclei the P2X(7)R is also expressed by subpopulations of cholinergic and GABAergic/glycinergic terminals. These data support our previous hypothesis that the P2X(7)R may play a role in modulating glutamate release in functionally different systems throughout the CNS but further suggest a role in modulating release of inhibitory transmitters in some regions.
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P2X7R may be co-localized in glutamatergic and GABAergic terminals.
“Dual labeling immunoreactivity studies in rats indicate that P2X7R may be co-localized in glutamatergic and GABAergic terminals [98].”
From P2X7 Receptor Signaling in Stress and Depression (2019) · cites this paper as [98]
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- Authors
- Atkinson, L., Batten, T. F. C., Moores, T. S., Varoqui, H., Erickson, J. D., & Deuchars, J.
- Affiliations
- University of Leeds (GB)
- Louisiana State University (US)
- Corresponding author
- Deuchars, J
- Venue
- Neuroscience
- Publisher
- Elsevier BV
- Published
- 2004-01
- Volume
- 123
- Issue
- 3
- Pages
- 761-768
- ISSN
- 0306-4522 (ISSN-L)
- OpenAlex
- W2002054197
- Cited by
- 80
- References
- 22
- Citations by year
- 2026: 1 · 2024: 1 · 2023: 4 · 2022: 2 · 2021: 2 · 2020: 3 · 2019: 2 · 2018: 1 · 2017: 2 · 2016: 1 · 2015: 2 · 2014: 4 · 2013: 5 · 2012: 6
- FWCI
- 3.74
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Neuroscience (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Physiology › Adenosine and Purinergic Signaling
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Health Sciences › Medicine › Physiology › Pain Mechanisms and Treatments
- Keywords
- vesicular glutamate transporter, VGLUT1, VGLUT2, colocalization, glutamatergic terminals, presynaptic receptors, glutamate release, inhibitory neurotransmission, cholinergic nerve terminals, GABAergic terminals, spinal cord, brainstem, cerebellum, hippocampus
- MeSH terms
- Animals, Brain, Brain Chemistry, Carrier Proteins, Spinal Cord, Rats, Wistar, Presynaptic Terminals, Receptors, Purinergic P2, Membrane Transport Proteins, Vesicular Transport Proteins, Vesicular Glutamate Transport Protein 1, Vesicular Glutamate Transport Protein 2, Rats, Receptors, Purinergic P2X7
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