Nicotine causes age-dependent changes in gene expression in the adolescent female rat brain
Abstract
Humans often start smoking during adolescence. Recent results suggest that rodents may also be particularly vulnerable to nicotine dependence during adolescence. We examined the effect of chronic nicotine exposure on gene expression profiles during adolescence in female rats, who were dosed with nicotine (and control animals were dosed with saline) via subcutaneously implanted osmotic minipumps. Brain samples were collected at four ages: before puberty (postnatal day 25), at about the time of puberty in females (postnatal day 35), and after puberty (postnatal days 45 and 55). The expression of 7931 genes in three brain areas was measured using DNA microarrays. Quantitative RT-PCR was also employed to confirm the expression patterns of selected genes. We used a novel clustering technique (principal cluster analysis) to classify 162 nicotine-regulated genes into five clusters, of which only one (cluster A) showed similar patterns of gene expression across all three brain areas (ventral striatum, prefrontal cortex, and hippocampus). Three clusters of genes (A, B, and C) showed dramatic peaks in their nicotine responses at the same age (p35). The other two clusters (D1 and D2) showed smaller peaks and/or valleys in their nicotine responses at p35 and p45. Thus, the age of maximal gene expression response to nicotine in female rats corresponds approximately to the age of maximal behavioral response and the age of puberty.
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The adolescent PFC has its maximal nicotine response in gene regulation for vesicle release, signal transduction, cytoskeleton dynamics, and transcription.
“The adolescent PFC shows maximal nicotine response in gene regulation involved in vesicle release, signal transduction, cytoskeleton dynamics, and transcription”
From Short- and Long-Term Consequences of Nicotine Exposure during Adolescence for Prefrontal Cortex Neuronal Network Function (2012) · cites this paper as Polesskaya et al. 2007
Adolescent exposure causes stronger PFC gene-expression changes than exposure during other developmental periods or adulthood.
“Animal studies have shown that exposure during adolescence induces stronger changes in gene expression in the PFC than during other periods of development and adulthood (Schochet et al. 2005, 2008; Polesskaya et al. 2007).”
From Short- and Long-Term Consequences of Nicotine Exposure during Adolescence for Prefrontal Cortex Neuronal Network Function (2012) · cites this paper as Polesskaya et al. 2007
Chronic nicotine exposure initiates long-term structural and functional adaptations.
“suggesting the role of chronic nicotine exposure in initiating long-term structural and functional adaptations (Polesskaya et al. 2007).”
From Short- and Long-Term Consequences of Nicotine Exposure during Adolescence for Prefrontal Cortex Neuronal Network Function (2012) · cites this paper as Polesskaya et al. 2007
Cites
Structural maturation of neural pathways in children and adolescents: in vivo study
Paus, T., Zijdenbos, A., Worsley, K., Collins, D. L., Blumenthal, J., Giedd, J. N., Rapoport, J. L., & Evans, A. C. (1999) · Science · doi:10.1126/science.283.5409.1908 · Cited by 1,322 · see citing works
Age-dependent effects of nicotine on locomotor activity and conditioned place preference in rats
Belluzzi, J., Lee, A., Oliff, H., & Leslie, F. (2004) · Psychopharmacology · doi:10.1007/s00213-003-1758-6 · Cited by 280 · see citing works
Evidence for enhanced neurobehavioral vulnerability to nicotine during periadolescence in rats
Adriani, W., Spijker, S., Deroche-Gamonet, V., Laviola, G., Le Moal, M., Smit, A. B., & Piazza, P. V. (2003) · The Journal of Neuroscience · doi:10.1523/jneurosci.23-11-04712.2003 · Cited by 257 · see citing works
Puberty and the onset of substance use and abuse
Patton, G. C., McMorris, B. J., Toumbourou, J. W., Hemphill, S. A., Donath, S., & Catalano, R. F. (2004) · Pediatrics · doi:10.1542/peds.2003-0626-f · Cited by 254 · see citing works
Nicotine-induced conditioned place preference in adolescent and adult rats
Vastola, B. J., Douglas, L. A., Varlinskaya, E. I., & Spear, L. P. (2002) · Physiology & Behavior · doi:10.1016/s0031-9384(02)00818-1 · Cited by 251 · see citing works
Bühring, H.-J., Streble, A., & Valent, P. (2004) · International Archives of Allergy and Immunology · doi:10.1159/000077351 · Cited by 231 · see citing works
Diminished nicotine withdrawal in adolescent rats: implications for vulnerability to addiction
O’Dell, L. E., Bruijnzeel, A. W., Smith, R. T., Parsons, L. H., Merves, M. L., Goldberger, B. A., Richardson, H. N., Koob, G. F., & Markou, A. (2006) · Psychopharmacology · doi:10.1007/s00213-006-0383-6 · Cited by 153 · see citing works
Self-citationSchochet, T. L., Kelley, A. E., & Landry, C. F. (2005) · Neuroscience · doi:10.1016/j.neuroscience.2005.05.057 · Cited by 93 · see citing works
Neurochemical alterations produced by daily nicotine exposure in periadolescent vs. adult male rats
Collins, S. L., Wade, D., Ledon, J., & Izenwasser, S. (2004) · European Journal of Pharmacology · doi:10.1016/j.ejphar.2004.08.039 · Cited by 62 · see citing works
Developmental differences in nicotine place conditioning
TORRELLA, T. A., BADANICH, K. A., PHILPOT, R. M., KIRSTEIN, C. L., & WECKER, L. (2004) · Annals of the New York Academy of Sciences · doi:10.1196/annals.1308.052 · Cited by 48 · see citing works
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Goriounova, N. A., & Mansvelder, H. D. (2012) · Cold Spring Harbor Perspectives in Medicine · doi:10.1101/cshperspect.a012120 · Cited by 307 · see citing works
“The adolescent PFC shows maximal nicotine response in gene regulation involved in vesicle release, signal transduction, cytoskeleton dynamics, and transcription, suggesting the role of chronic nicotine exposure in initiating longterm structural and functional adaptations (Polesskaya et al. 2007).”
BackgroundMoylan, S., Jacka, F. N., Pasco, J. A., & Berk, M. (2013) · Brain and Behavior · doi:10.1002/brb3.137 · Cited by 186 · see citing works
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Korpi, E. R., den Hollander, B., Farooq, U., Vashchinkina, E., Rajkumar, R., Nutt, D. J., Hyytiä, P., & Dawe, G. S. (2015) · Pharmacological Reviews · doi:10.1124/pr.115.010967 · Cited by 148 · see citing works
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Poorthuis, R. B., Goriounova, N. A., Couey, J. J., & Mansvelder, H. D. (2009) · Biochemical Pharmacology · doi:10.1016/j.bcp.2009.04.031 · Cited by 102 · see citing works
Rodent models of nicotine withdrawal syndrome
Malin, D. H., & Goyarzu, P. (2009) · Handbook of experimental pharmacology · doi:10.1007/978-3-540-69248-5_14 · Cited by 89 · see citing works
Eppolito, A. K., Bachus, S. E., McDonald, C. G., Meador-Woodruff, J. H., & Smith, R. F. (2010) · Neurotoxicology and Teratology · doi:10.1016/j.ntt.2009.12.009 · Cited by 69 · see citing works
Self-citationGoriounova, N. A., & Mansvelder, H. D. (2012) · Frontiers in Synaptic Neuroscience · doi:10.3389/fnsyn.2012.00003 · Cited by 65 · see citing works
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Spaeth, A. M., Barnet, R. C., Hunt, P. S., & Burk, J. A. (2010) · Pharmacology Biochemistry and Behavior · doi:10.1016/j.pbb.2010.07.011 · Cited by 29 · see citing works
Eddins, D., Petro, A., & Levin, E. D. (2022) · Neurotoxicology and Teratology · doi:10.1016/j.ntt.2022.107122 · Cited by 2 · see citing works
- Authors
- Polesskaya, O. O., Fryxell, K. J., Merchant, A. D., Locklear, L. L., Ker, K.-F., McDonald, C. G., Eppolito, A. K., Smith, L. N., Wheeler, T. L., & Smith, R. F.
- Affiliations
- George Mason University (US)
- Corresponding author
- Fryxell, Karl J.
- Venue
- Neurotoxicology and Teratology
- Publisher
- Elsevier BV
- Published
- 2007-01
- Volume
- 29
- Issue
- 1
- Pages
- 126-140
- ISSN
- 0892-0362 (ISSN-L)
- OpenAlex
- W2069099310
- Cited by
- 36
- References
- 10
- Citations by year
- 2024: 1 · 2023: 1 · 2022: 4 · 2021: 4 · 2020: 1 · 2017: 1 · 2016: 2 · 2015: 3 · 2014: 2 · 2013: 3 · 2012: 3
- FWCI
- 1.33
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Neurotoxicology and Teratology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- George Mason University
- Topics
- Health Sciences › Medicine › Physiology › Smoking Behavior and Cessation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nicotinic Acetylcholine Receptors Study
- Health Sciences › Medicine › Pediatrics, Perinatology and Child Health › Prenatal Substance Exposure Effects
- Keywords
- nicotine, adolescent brain, gene expression, puberty, female rats, nicotine dependence, chronic nicotine exposure, ventral striatum, prefrontal cortex, hippocampus, smoking initiation
- MeSH terms
- Age Factors, Aging, Animals, Animals, Newborn, Brain, Female, Nicotine, Reproducibility of Results, Cluster Analysis, Gene Expression Regulation, Developmental, Nicotinic Agonists, Reverse Transcriptase Polymerase Chain Reaction, Rats, Long-Evans, Oligonucleotide Array Sequence Analysis, Rats
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