Cited by 90Mosquito-borne diseases and controlSARS-CoV-2 and COVID-19 ResearchRNA Interference and Gene Delivery

Modified mRNA-LNP Vaccines Confer Protection against Experimental DENV-2 Infection in Mice

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Zhang, Mengling, Sun, Jin, Li, Min, Jin, Xia·2020·Molecular Therapy - Methods & Clinical Development

Abstract

Dengue virus (DENV) infection is a major global public health concern, and there is no effective vaccine for it. In this study, we describe the design and characterization of three nucleotide-modified mRNA vaccines (prME-mRNA, E80-mRNA, and NS1-mRNA) for DENV-2. Our results showed that vaccination with E80-mRNA alone or a combination of E80-mRNA and NS1-mRNA can induce high levels of neutralizing antibodies and antigen-specific T cell responses; furthermore, these vaccines confer complete protection against DENV-2 challenge in immunocompetent mice. These data provide foundations for further development of a tetravalent DENV vaccine based on nucleotide-modified mRNA.

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Crossref · 10.1016/j.omtm.2020.07.013

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  • Lipid nanoparticle-mRNA vaccines have been tested against other viruses in animal models, including human immunodeficiency virus, Powassan virus, Venezuelan equine encephalitis virus, dengue virus and Ebola virus.

    “Furthermore, lipid nanoparticle–mRNA vaccines have been tested against other viruses in animal models, including human immunodeficiency virus28,144,181,190, Powassan virus195, Venezuelan equine encephalitis virus196, dengue virus73 and Ebola virus197.”

    From Lipid nanoparticles for mRNA delivery (2021) · cites this paper as [73]

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Authors
Zhang, M., Sun, J., Li, M., & Jin, X.
Affiliations
  • Chinese Academy of Sciences (CN)
  • Fudan University (CN)
Corresponding author
Jin, Xia
Venue
Molecular Therapy - Methods & Clinical Development
Publisher
Elsevier BV
Published
2020-09
Volume
18
Pages
702-712
ISSN
2329-0501 (ISSN-L)
OpenAlex
W3045088564
Cited by
90
References
58
Citations by year
2026: 14 · 2025: 23 · 2024: 21 · 2023: 9 · 2022: 13 · 2021: 10
FWCI
6.00
Type
journal article
Language
en
Open access
Locations
  • Molecular Therapy — Methods & Clinical Development (publishedVersion, cc-by-nc-nd)
  • PubMed Central (publishedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
National Natural Science Foundation of China
Topics
  • Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Mosquito-borne diseases and control
  • Health Sciences › Medicine › Infectious Diseases › SARS-CoV-2 and COVID-19 Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
Keywords
dengue virus type 2, mRNA vaccines, lipid nanoparticles, neutralizing antibodies, T cell response, tetravalent dengue vaccine, vaccine protection

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