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Cited by 48Advanced Glycation End Products researchGenomics, phytochemicals, and oxidative stressRetinal Diseases and Treatments

Bioactive compounds isolated from apple, tea, and ginger protect against dicarbonyl induced stress in cultured human retinal epithelial cells

Sampath, Chethan, Zhu, Yingdong, Sang, Shengmin, Ahmedna, Mohamed·2016·Phytomedicine
DOI 10.1016/j.phymed.2015.12.013
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Abstract

Background Methylglyoxal (MGO) is known to be a major precursor of advanced glycation end products (AGEs) which are linked to diabetes and its related complications. Naturally occurring bioactive compounds could play an important role in countering AGEs thereby minimizing the risk associated with their formation.Methods In this study, eight specific bioactive compounds isolated from apple, tea and ginger were evaluated for their AGEs scavenging activity using Human Retinal Pigment Epithelial (H-RPE) cells treated with MGO.Results Among the eight specific compounds evaluated, (-)-epigallocatechin 3-gallate (EGCG) from tea, phloretin in apple, and [6]-shogaol and [6]-gingerol from ginger were found to be most effective in preventing MGO-induced cytotoxicity in the epithelial cells. Investigation of possible underlying mechanisms suggests that that these compounds could act by modulating key regulative detoxifying enzymes via modifying nuclear factor-erythroid 2-related factor 2 (Nrf2) function. MGO-induced cytotoxicity led to increased levels of AGEs causing increase in Nε-(Carboxymethyl) lysine (CML) and glutathione (GSH) levels and over expression of receptor for advanced glycation end products (RAGE). Data also showed that translocation of Nrf2 from cytosol to nucleus was inhibited, which decreased the expression of detoxifying enzyme like heme oxygenase-1 (HO-1). The most potent bioactive compounds scavenged dicarbonyl compounds, inhibited AGEs formation and significantly reduced carbonyl stress by Nrf2 related pathway and restoration of HO-1 expression.Conclusions These findings demonstrated the protective effect of bioactive compounds derived from food sources against MGO-induced carbonyl stress through activation of the Nrf2 related defense pathway, which is of significant importance for therapeutic interventions in complementary treatment/management of diabetes-related complications.

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Crossref · 10.1016/j.phymed.2015.12.013

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  • In the presence of MGO, the translocation of Nrf2 from the cytosol to the nucleus is inhibited, which results in a decreased expression of detoxifying enzymes such as heme oxygenase-1.

    “In fact, in the presence of MGO, the translocation of Nrf2 from the cytosol to the nucleus is inhibited, which results in a decreased expression of detoxifying enzymes such as heme oxygenase-1 [195].”

    From GANAB and N-Glycans Substrates Are Relevant in Human Physiology, Polycystic Pathology and Multiple Sclerosis: A Review (2022) · cites this paper as [195]

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Authors
Sampath, C., Zhu, Y., Sang, S., & Ahmedna, M.
Affiliations
  • Qatar University (QA)
  • North Carolina Agricultural and Technical State University (US)
Corresponding author
Ahmedna, Mohamed
ORCID
Venue
Phytomedicine
Publisher
Elsevier BV
Published
2016-02
Volume
23
Issue
2
Pages
200-213
ISSN
0944-7113 (ISSN-L)
DOI
10.1016/j.phymed.2015.12.013
OpenAlex
W2229729265
Cited by
48
References
59
Citations by year
2026: 1 · 2025: 6 · 2024: 2 · 2023: 3 · 2022: 7 · 2021: 5 · 2020: 3 · 2019: 8 · 2018: 2 · 2017: 7 · 2016: 4
FWCI
6.91
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Phytomedicine (publishedVersion)
  • PubMed (publishedVersion)
  • Qatar University QSpace (Qatar University) (submittedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Clinical Biochemistry › Advanced Glycation End Products research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics, phytochemicals, and oxidative stress
  • Health Sciences › Medicine › Ophthalmology › Retinal Diseases and Treatments
Keywords
methylglyoxal, advanced glycation end products, carbonyl stress, Nrf2, heme oxygenase-1, retinal pigment epithelial cells, epigallocatechin gallate, phloretin, shogaol, gingerol, RAGE, glutathione, diabetes complications, apple, tea, ginger
MeSH terms
Receptor for Advanced Glycation End Products, Catechin, Catechols, Cell Line, Epithelial Cells, Fatty Alcohols, Glutathione, Humans, Lysine, Phloretin, Pyruvaldehyde, Tea, Glycation End Products, Advanced, Protective Agents, Zingiber officinale, Malus, NF-E2-Related Factor 2, Heme Oxygenase-1, Retinal Pigment Epithelium, Phytochemicals

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