Pituitary CRH-binding protein and stress in female mice

Stinnett, Gwen S.; Westphal, Nicole J.; Seasholtz, Audrey F.

doi:10.1016/j.physbeh.2015.02.050

Cited by 33Stress Responses and CortisolNeuropeptides and Animal PhysiologyAdrenal Hormones and Disorders

Pituitary CRH-binding protein and stress in female mice

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Stinnett, Gwen S., Westphal, Nicole J., Seasholtz, Audrey F.·2015·Physiology & Behavior

DOI 10.1016/j.physbeh.2015.02.050PMC PMC4546865

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Abstract

The CRH-binding protein (CRH-BP) binds CRH with very high affinity and inhibits CRH-mediated ACTH release from anterior pituitary cells in vitro, suggesting that the CRH-BP functions as a negative regulator of CRH activity. Our previous studies have demonstrated sexually dimorphic expression of CRH-BP in the murine pituitary. Basal CRH-BP expression is higher in the female pituitary, where CRH-BP mRNA is detected in multiple anterior pituitary cell types. In this study, we examined stress-induced changes in CRH-BP mRNA and protein expression in mouse pituitary and assessed the in vivo role of CRH-BP in modulating the stress response. Pituitary CRH-BP mRNA was greater than 200-fold more abundant in females than males, and restraint stress increased pituitary CRH-BP mRNA by 11.8-fold in females and 3.2-fold in males as assessed by qRT-PCR. In females, restraint stress increased CRH-BP mRNA levels not only in POMC-expressing cells, but also in PRL-expressing cells. The increase in female pituitary CRH-BP mRNA following stress resulted in significant increases in CRH-BP protein 4-6h after a 30-minute restraint stress as detected by [(125)I]-CRH:CRH-BP cross-linking analyses. Based on this temporal profile, the physiological role of CRH-BP was assessed using a stressor of longer duration. In lipopolysaccharide (LPS) stress studies, female CRH-BP-deficient mice showed elevated levels of stress-induced corticosterone release as compared to wild-type littermates. These studies demonstrate a role for the pituitary CRH-BP in attenuating the HPA response to stress in female mice.

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Female mice have higher pituitary Crh-BP mRNA transcripts than male mice, even after restraint stress.
“Stinnett et al. (2015), indicated higher Crh-BP mRNA transcripts in pituitary from female mice versus male mice, even after restraint stress”
From Hypothalamic–pituitary–adrenal and hypothalamic–pituitary–gonadal axes: sex differences in regulation of stress responsivity (2017) · cites this paper as Stinnett, Westphal, & Seasholtz, 2015

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Regulation of Pituitary Corticotropin-Releasing Hormone-Binding Protein Messenger Ribonucleic Acid Levels by Restraint Stress and Adrenalectomy**This work is supported by National Institutes of Health Grant DK-42730 (to A.F.S) and by a Young Investigator Award (to A.F.S.) from the National Alliance for Research on Schizophrenia and Depression.
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Self-citation

Authors: Stinnett, G. S., Westphal, N. J., & Seasholtz, A. F.
Affiliations: University of Michigan (US)
Corresponding author: Seasholtz, Audrey F.
ORCID: Stinnett, Gwen S.
Seasholtz, Audrey F.
Venue: Physiology & Behavior
Publisher: Elsevier BV
Published: 2015-10
Volume: 150
Pages: 16-23
ISSN: 0031-9384 (ISSN-L)
DOI: 10.1016/j.physbeh.2015.02.050
PMC: PMC4546865
OpenAlex: W2085518231
Cited by: 33
References: 59
Citations by year: 2026: 4 · 2025: 3 · 2024: 4 · 2022: 5 · 2021: 2 · 2020: 2 · 2019: 1 · 2018: 3 · 2017: 6 · 2016: 2 · 2015: 1
FWCI: 2.08
Type: journal article
Language: en
Open access: green
www.ncbi.nlm.nih.gov
Licence: www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations: Physiology & Behavior (publishedVersion)
PubMed (publishedVersion)
Europe PMC (PubMed Central) (acceptedVersion)
PubMed Central (acceptedVersion)
Funders: NRSA (048775)
NIH (DK42730, DK57660)
University of Michigan Medical School
Topics: Life Sciences › Neuroscience › Behavioral Neuroscience › Stress Responses and Cortisol
Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuropeptides and Animal Physiology
Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Adrenal Hormones and Disorders
Keywords: CRH-binding protein, corticotropin-releasing factor, anterior pituitary, restraint stress, lipopolysaccharide, hypothalamic-pituitary-adrenal axis, corticosterone release, sex differences, lactotrophs, stress response regulation
MeSH terms: Adrenocorticotropic Hormone, Animals, Carrier Proteins, Corticosterone, Corticotropin-Releasing Hormone, Female, Gene Expression Regulation, Iodine Isotopes, Lipopolysaccharides, Male, Mice, Inbred C57BL, Mice, Transgenic, Pituitary Gland, RNA, Messenger, Sex Characteristics, Stress, Physiological, Time Factors, Mice

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Pituitary CRH-binding protein and stress in female mice

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