Cited by 63Retinal Development and DisordersPhotoreceptor and optogenetics researchRetinoids in leukemia and cellular processes

Membrane-binding and enzymatic properties of RPE65

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Kiser, Philip D., Palczewski, Krzysztof·2010·Progress in Retinal and Eye Research

Abstract

Regeneration of visual pigments is essential for sustained visual function. Although the requirement for non-photochemical regeneration of the visual chromophore, 11-cis-retinal, was recognized early on, it was only recently that the trans to cis retinoid isomerase activity required for this process was assigned to a specific protein, a microsomal membrane enzyme called RPE65. In this review, we outline progress that has been made in the functional characterization of RPE65. We then discuss general concepts related to protein-membrane interactions and the mechanism of the retinoid isomerization reaction and describe some of the important biochemical and structural features of RPE65 with respect to its membrane-binding and enzymatic properties.

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Crossref · 10.1016/j.preteyeres.2010.03.002

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  • 11-cis-retinal is produced in the RPE and delivered to photoreceptors.

    “The photosensitive active retinoid, 11-cis-retinal, is produced in the RPE and delivered to the photoreceptors.”

    From Chemistry of the Retinoid (Visual) Cycle (2014) · cites this paper as [284]

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Authors
Kiser, P. D., & Palczewski, K.
Affiliations
Case Western Reserve University (US)
Corresponding author
Palczewski, Krzysztof
Venue
Progress in Retinal and Eye Research
Publisher
Elsevier BV
Published
2010-09
Volume
29
Issue
5
Pages
428-442
ISSN
1350-9462 (ISSN-L)
OpenAlex
W2118546994
Cited by
62
References
120
Citations by year
2026: 1 · 2025: 1 · 2024: 2 · 2023: 2 · 2022: 3 · 2021: 3 · 2020: 4 · 2019: 4 · 2018: 2 · 2017: 5 · 2016: 3 · 2015: 5 · 2014: 8 · 2013: 6 · 2012: 7
FWCI
1.07
Type
journal article
Language
en
Open access
Locations
  • Progress in Retinal and Eye Research (publishedVersion)
  • PubMed (publishedVersion)
  • Europe PMC (PubMed Central) (acceptedVersion)
  • PubMed Central (acceptedVersion)
Funders
National Eye Institute (T32EY007157, R01EY009339)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Retinal Development and Disorders
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Photoreceptor and optogenetics research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Retinoids in leukemia and cellular processes
Keywords
RPE65, 11-cis-retinal, visual pigment regeneration, protein-membrane interactions, membrane binding
MeSH terms
Retinoid Isomerohydrolase, Animals, Carrier Proteins, Eye Proteins, Humans, Protein Binding, cis-trans-Isomerases, Retinal Pigment Epithelium, Leber Congenital Amaurosis

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