Cited by 99Probiotics and Fermented FoodsAnimal Nutrition and PhysiologySalmonella and Campylobacter epidemiology

Protection activity of a novel probiotic strain of Bacillus subtilis against Salmonella Enteritidis infection

Thirabunyanon, Mongkol, Thongwittaya, Narin·2012·Research in Veterinary Science

Abstract

The activity of 240 bacterial isolates screened from the gastrointestinal tracts of native chickens were evaluated for use as a potential probiotic in food animal production in order to protect against animal diseases and reduce pathogenic contamination of human food products. In observing the antagonistic activity of 117 bacilli isolates, 10 of these isolates exhibited higher growth inhibition of seven foodborne pathogens, including Salmonella Enteritidis, Salmonella Typhimurium, Escherichia coli, Bacillus cereus, Staphylococcus aureus, Listeria monocytogenes, and Vibrio cholerae. Beneficial probiotic criteria from these isolates - which included non-pathogenicity, acid and bile salt tolerance, hydrophobicity, and adhesion to intestinal epithelial cells - exhibited that one isolate of NC11 had the most potential as a probiotic. 16S rRNA gene sequencing showed that this NC11 isolate was Bacillus subtilis. This B. subtilis NC11 was sensitive to all antibiotics and was not cytotoxic to intestinal epithelial cells. Reduction of S. Enteritidis attachment to the surfaces of intestinal epithelial cells via action of a cultured medium from B. subtilis NC11 was observed by scanning electron microscopy. B. subtilis NC11 cells, as well as the bacterial cultured medium or the cultured medium adjusted to pH 7, significantly inhibited S. Enteritidis invasion (P<0.01) of intestinal epithelial cells. This study indicates that B. subtilis NC11 has characteristics of a potential probiotic, and exhibits strong inhibition activity against S. Enteritidis infection to intestinal epithelial cells.

Integrity signals

Registry

Crossref · 10.1016/j.rvsc.2011.08.008

Cites

38 of 38 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Cites

Flagged first, then by citations

Authors
Thirabunyanon, M., & Thongwittaya, N.
Affiliations
Maejo University (TH)
Corresponding author
Thirabunyanon, Mongkol
Venue
Research in Veterinary Science
Publisher
Elsevier BV
Published
2012-08
Volume
93
Issue
1
Pages
74-81
ISSN
0034-5288 (ISSN-L)
OpenAlex
W2002249097
Cited by
99
References
38
Citations by year
2026: 2 · 2025: 2 · 2024: 5 · 2023: 9 · 2022: 12 · 2021: 9 · 2020: 9 · 2019: 5 · 2018: 13 · 2017: 9 · 2016: 5 · 2015: 6 · 2014: 6 · 2013: 5 · 2012: 2
FWCI
6.08
Type
journal article
Language
en
Open access
closed
Locations
  • Research in Veterinary Science (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Thailand Research Fund
  • National Research Council of Thailand
Topics
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
  • Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Nutrition and Physiology
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Salmonella and Campylobacter epidemiology
Keywords
Bacillus subtilis, probiotics, Salmonella Enteritidis, antibacterial activity, chickens, intestinal epithelial cells, bacterial adhesion, bacterial invasion, foodborne pathogens, antagonistic activity, acid and bile tolerance, hydrophobicity, antibiotic susceptibility
MeSH terms
Animals, Bacillus subtilis, Cells, Cultured, Chickens, Intestinal Mucosa, Microscopy, Electron, Scanning, Poultry Diseases, RNA, Ribosomal, 16S, Salmonella enteritidis, Salmonella Infections, Animal, Sequence Analysis, DNA, Probiotics

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review