Somaclonal variation among somatic embryo derived plants—Evaluation of agronomically important somaclones and detection of genetic changes by RAPD in Cymbopogon winterianus
Open accessAbstract
A total of one hundred five plants of Cymbopogon winterianus derived from indirect somatic embryogenesis were established in the field. Extensive somaclonal variation was noticed within these plants for all eight agronomic characters such as plant height, diameter of bush, number of tiller/clump, number of leaves/clump, leaf length, leaf breadth, weight of 100 leaves and oil content in relation to the donor parent. Significant variations were also recorded for two major constituents of essential oil such as citronellal and geraniol in some selected somaclones. Ten somaclones that were retained improved oil content and quality in initial screening and, in a replicated trial, were further assessed for their stability in the field for four clonal propagations. Out of the ten superior somaclones only five superior somaclones (SC1, SC2, SC3, SC6 and SC10) which showed relative stability both in oil content and quality were subjected to random amplified polymorphic DNA (RAPD) analysis. Out of the eighteen primers used, ten primers revealed polymorphism showing distinctly different banding patterns in the five improved somaclones, which were equally prominent in their differences from the control. Out of the 44 polymorphic bands, 30 were parental bands which were missing from some somaclonal progenies and 14 were novel non-parental bands which were detected only in some somaclones that confirmed the presence of genetic changes due to somaclonal variation.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In Cymbopogon winterianus, somaclones with high agronomic characters and citronellal and geraniol essential oil content were identified.
“in 2015, Dey et al. [64] identified Cymbopogon winterianus somaclones with high agronomic characters (plant height, diameter of bush, number of tiller/clump, number of leaves/clump, leaf length, leaf breadth, weight of 100 leaves, and citronellal and geraniol essential oil content).”
From Somaclonal Variation—Advantage or Disadvantage in Micropropagation of the Medicinal Plants (2023) · cites this paper as Dey et al. [64]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Somaclonal Variation in Plants: Causes and Detection Methods
Bairu, M. W., Aremu, A. O., & Van Staden, J. (2011) · Plant Growth Regulation · doi:10.1007/s10725-010-9554-x · Cited by 674 · see citing works
Kukreja, A. K., Dhawan, O. P., Mathur, A. K., Ahuja, P. S., & Mandal, S. (1991) · Euphytica · doi:10.1007/bf00023270 · Cited by 28 · see citing works
Flagged first, then by citations
Research works printed but not linked: 17
- Abdellatif, M.F., Hegazy, A.E., Aboshama, H.M., Emara, H.A., El-Shahed, A.A., 2012. Morphological and molecular characterization of somaclonal variations in tissue culture-derived banana plants. Journal of Genetic Engineering and Biotechnology 10, 47-53.
- Aruingtyas, E.L., Widoretno, W., Idriyani, S., 2012. Somaclonal variation of soybean (Glysin max. L. Merr) stimulated by draught stress based on Random Amplified Polymorphic DNA (RAPDs). American Journal of Molecular Biology 2, 85-91.
- Bajaj, Y.P.S., 1990. Somaclonal variation-origin, induction, cryopreservation and implica- tion in plant breeding. In: Bajaj, Y.P.S. (Ed.), Biotechnology in Agriculture and Forestry 11Somaclonal Variation in Crop Improvement. Springer-Verlag, Berlin, Heidelberg, New York, pp. 3-48.
- Clevenger, J.F., 1928. Apparatus for determination of volatile oil. Journal of the American Pharmaceutical Association 17, 346.
- Dey, T., Bhattacharya, S., Ghosh, P.D., 2010a. Somatic embryogenesis from rhizome explants of Cymbopogon winterianus. Biologia Plantarum 54 (2), 325-328.
- Dey, T., Saha, S., Dhar, T.N., Adhikary, S., Ghosh, P.D., 2010b. Optimization and comparison of efficiency between two DNA isolation protocols in Cymbopogon species. General and Applied Plant Physiology 36 (3-4), 232-238.
- Guenther, E., 1972. The Essential Oils vol. I. Robert E. KriegerPubl Co, New York, Huntington, pp. 316-319.
- Jaccard, P., 1908. Nouvelles recherches sur la distribution florale. Bulletin de la SosiétéVaudoise Sciences Naturelles 44, 223-270.
- Kukreja, A.K., Dhawan, O.P., Ahuja, P.S., Sharma, S., Mathur, A.K., 1992. Genetic improve- ments of mints: on the qualitative traits of essential oil of in vitro derived clones of Japanese mint (Mentha arvensis var. piperascens Holms). Journal of Essential Oil Research 4, 623-629.
- Ngezahayo, F., Dong, Y., Liu, B., 2007. Somaclonal variation at the nucleotide sequence level in rice (Oryza sativa L.) as revealed by RAPD and ISSR markers and by pairwise sequence analysis. Journal of Applied Genetics 48 (4), 329-336.
- Osipova, E.S., Kokaeva, Z.G., Troitskij, A.V., Dolgikh, Y.I., Shamina, Z.B., Gotimskij, S.A., 2001. RAPD analysis of maize somaclones. Genetika-Moskva 37 (1), 91-96.
- Patnaik, J., Debata, B.K., 2001. RAPD analysis of variant somaclones of palmarosa (Cymbopogon martinii var. motia). In: Sahoo, S., Ramesh, D.B., Rao, Y.R., Debata, B.K., Misra, V. (Eds.), Conservation and Utilization of Medicinal and Aromatic Plants. RRL, Bhubaneswar, Allied Publishers Ltd, New Delhi, pp. 296-301.
- Peyvandi, M., Farahzadi, H.N., Arbabian, S., Noormohammadi, Hosseini-Mazinami, 2010. Somaclonal variation among somatic embryo derived plants of Olea europaea L "cv. Kroneiki". Journal of Sciences, Islamic Republic of Iran 21 (1), 7-14.
- Saker, M.M., Bekheet, S.A., Taha, H.S., Fahmy, A.S., Moursy, H.A., 2000. Detection of somaclonal variation in tissue culture derived date palm plants using isoenzyme analysis and RAPD fingerprints. Biologia Plantarum 43 (3), 347-351.
- Sreenath, H.L., Jagadishchandra, K.S., 1991. Cymbopogon spreng (aromatic grasses) in vitro culture, regeneration and production of essential oils. In: Bajaj, Y.P.S. (Ed.), Biotechnology in Agriculture and ForestryMedicinal and Aromatic Plants III vol. 15. Springer-Verlag, Berlin-Heidelberg, New York, pp. 211-236.
- Tawar, P.N., Sawant, R.A., Dalvi, S.G., Nikam, A.A., Tawar, P.G., Devarumath, R.M., 2008. An assessment of somaclonal variation in micropropagated plants of sugarcane by RAPD markers. Sugar Technology 10 (2), 124-127.
- Viehmannova, I., Bortlova, Z., Vitamvas, J., Cepkova, P.H., Eliasova, K., Svobodova, E., Travnickova, M., 2014. Assessment of somaclonal variation in somatic embryo derived plants of Yacon [Smallanthus sonchifolius (Poepp. and Endl.) H. Robinson] using inter simple sequence repeat analysis and flow cytometry. Electronic Journal of Biotechnology http://dx.doi.org/10.1016/j.ejbt.2013.12.011. · DOI
Cited by
Sorted by citations
Somaclonal variations and their applications in horticultural crops improvement
Krishna, H., Alizadeh, M., Singh, D., Singh, U., Chauhan, N., Eftekhari, M., & Sadh, R. K. (2016) · 3 Biotech · doi:10.1007/s13205-016-0389-7 · Cited by 436 · see citing works
Somaclonal Variation—Advantage or Disadvantage in Micropropagation of the Medicinal Plants
Duta-Cornescu, G., Constantin, N., Pojoga, D.-M., Nicuta, D., & Simon-Gruita, A. (2023) · International Journal of Molecular Sciences · doi:10.3390/ijms24010838 · Cited by 108 · see citing works
“[64] identified Cymbopogon winterianus somaclones with high agronomic characters (plant height, diameter of bush, number of tiller/clump, number of leaves/clump, leaf length, leaf breadth, weight of 100 leaves, and citronellal and geraniol essential oil content).”
BackgroundBiswas, P., & Kumar, N. (2023) · Molecular Marker Techniques · doi:10.1007/978-981-99-1612-2_12 · Cited by 29 · see citing works
CHAVEZ-CORTAZAR, A., MATA-ROSAS, M., OYAMA, K., SAMAIN, M. S., & QUESADA, M. (2020) · Biologia plantarum · doi:10.32615/bp.2020.006 · Cited by 12 · see citing works
- Authors
- Dey, T., Saha, S., & Ghosh, P. D.
- Affiliations
- University of Kalyani (IN)
- Corresponding author
- Dey, T.
- ORCID
- Venue
- South African Journal of Botany
- Publisher
- Elsevier BV
- Published
- 2015-01
- Volume
- 96
- Pages
- 112-121
- ISSN
- 0254-6299 (ISSN-L)
- OpenAlex
- W2067920289
- Cited by
- 38
- References
- 2
- Citations by year
- 2026: 2 · 2025: 4 · 2024: 1 · 2023: 8 · 2022: 3 · 2021: 4 · 2020: 4 · 2019: 5 · 2018: 2 · 2017: 2 · 2016: 2 · 2015: 1
- FWCI
- 0.68
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- South African Journal of Botany (publishedVersion, cc-by)
- Funders
- University Grants Commission (UGC), New Delhi, India (F-30-140/2004)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant tissue culture and regeneration
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Genetics and Plant Breeding
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Genetic and Mutation Studies
- Keywords
- somaclonal variation, Cymbopogon winterianus, Cymbopogon citratus, somatic embryogenesis, somaclones, essential oil content, citronellal, geraniol, agronomic traits, clonal stability, RAPD polymorphism, genetic alterations
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
