Individual and combined multigenerational effects induced by polystyrene nanoplastic and glyphosate in Daphnia magna (Strauss, 1820)
Abstract
In this study, the acute and multigenerational effects of the individual and combined toxicity of polystyrene nanoplastic (PSNP - 15.6, 31.2 62.5, 125, 250 and 500 mg/L) and glyphosate (Gly - 6.2, 12.5, 25, 50, 100 and 200 mg/L) on the freshwater crustacean Daphnia magna were investigated. The acute toxicity interactions were predicted mathematically using Abbott's model and multiple toxicological endpoints. In the multigenerational tests, we evaluated the effects in filial (F1 and F2) generations of daphnids after parental (F0) exposure to Gly and PSNP, as individual compounds and as a mixture, during their life history. Based on Abbott's model, the combined individual toxicities of Gly and PSNP are increased when they are present as a mixture. This indicates synergy between the components of the mixture, especially in the case of co-exposure to Gly and PSNP in higher equitoxic proportions. The mixture of PSNP and Gly caused an increase in immobility and ROS production and decrease in swimming activity. Multigenerational responses indicated that the exposure of F0 daphnids to Gly and PSNP as a mixture induced effects in the F1 and F2 reproduction parameters in the recovery tests. Thus, the results reported herein provide important information on the interaction of hydrophilic organic and nanoplastic pollutants in aqueous ecosystems. This will be useful in future studies on the toxicity of mixtures and multigenerational effects and provide a basis for management decisions aimed at the protection of environmental health.
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The increase in distance traveled, particularly under noncorrected pH and at intermediate concentrations such as 7 g L -1, is consistent with stress-induced hyperactivity.
“The increase in distance traveled, particularly under noncorrected pH and at intermediate concentrations such as 7 g L -1 , is consistent with stress-induced hyperactivity, often interpreted as an escape or avoidance response to unfavorable environmental conditions [97].”
From Wildfire Ash Alters Freshwater Crustacean Behavior: Disentangling the Contributions of Suspended Macroparticles and Ash‐Induced Alkalinization (2026) · cites this paper as [97]
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Aquatic ecotoxicity of glyphosate, its formulations, and co-formulants: evidence from 2010 to 2023
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Self-citation
- Authors
- Nogueira, D. J., Silva, A. C. D. O. D., da Silva, M. L. N., Vicentini, D. S., & Matias, W. G.
- Affiliations
- Universidade Federal de Santa Catarina (BR)
- Corresponding author
- Matias, William Gerson
- Venue
- Science of The Total Environment
- Publisher
- Elsevier BV
- Published
- 2022-03
- Volume
- 811
- Pages
- 151360
- ISSN
- 0048-9697 (ISSN-L)
- OpenAlex
- W3212000163
- Cited by
- 74
- References
- 65
- Citations by year
- 2026: 19 · 2025: 11 · 2024: 17 · 2023: 18 · 2022: 9
- FWCI
- 2.73
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- The Science of The Total Environment (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Conselho Nacional de Desenvolvimento Científico e Tecnológico
- Universidade Federal de Santa Catarina
- Topics
- Physical Sciences › Environmental Science › Pollution › Microplastics and Plastic Pollution
- Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Environmental Toxicology and Ecotoxicology
- Physical Sciences › Environmental Science › Pollution › Pharmaceutical and Antibiotic Environmental Impacts
- Keywords
- glyphosate, Daphnia magna, multigenerational effects, combined toxicity, synergistic toxicity, acute toxicity, reproduction, reactive oxygen species, swimming activity, immobility, freshwater crustaceans, aquatic ecotoxicology
- MeSH terms
- Microplastics, Glyphosate, Animals, Daphnia, Glycine, Polystyrenes, Reproduction, Water Pollutants, Chemical, Ecosystem
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