The DOI matches its Crossref record; 48 of the 48 references it cites were found in a registry or an index.
Esrrb Is a Direct Nanog Target Gene that Can Substitute for Nanog Function in Pluripotent Cells
Open accessAbstract
Embryonic stem cell (ESC) self-renewal efficiency is determined by the level of Nanog expression. However, the mechanisms by which Nanog functions remain unclear, and in particular, direct Nanog target genes are uncharacterized. Here we investigate ESCs expressing different Nanog levels and Nanog(-/-) cells with distinct functionally inducible Nanog proteins to identify Nanog-responsive genes. Surprisingly, these constitute a minor fraction of genes that Nanog binds. Prominent among Nanog-reponsive genes is Estrogen-related receptor b (Esrrb). Nanog binds directly to Esrrb, enhances binding of RNAPolII, and stimulates Esrrb transcription. Overexpression of Esrrb in ESCs maintains cytokine-independent self-renewal and pluripotency. Remarkably, this activity is retained in Nanog(-/-) ESCs. Moreover, Esrrb can reprogram Nanog(-/-) EpiSCs and can rescue stalled reprogramming in Nanog(-/-) pre-iPSCs. Finally, Esrrb deletion abolishes the defining ability of Nanog to confer LIF-independent ESC self-renewal. These findings are consistent with the functional placement of Esrrb downstream of Nanog.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Takahashi, K., & Yamanaka, S. (2006) · Cell · doi:10.1016/j.cell.2006.07.024 · Cited by 26,673
Core Transcriptional Regulatory Circuitry in Human Embryonic Stem Cells
Boyer, L. A., Lee, T. I., Cole, M. F., Johnstone, S. E., Levine, S. S., Zucker, J. P., Guenther, M. G., Kumar, R. M., Murray, H. L., Jenner, R. G., et al. (2005) · Cell · doi:10.1016/j.cell.2005.08.020 · Cited by 4,505
Niwa, H., Miyazaki, J.-I., & Smith, A. G. (2000) · Nature Genetics · doi:10.1038/74199 · Cited by 3,596
The ground state of embryonic stem cell self-renewal
Ying, Q.-L., Wray, J., Nichols, J., Batlle-Morera, L., Doble, B., Woodgett, J., Cohen, P., & Smith, A. (2008) · Nature · doi:10.1038/nature06968 · Cited by 3,442
Nichols, J., Zevnik, B., Anastassiadis, K., Niwa, H., Klewe-Nebenius, D., Chambers, I., Schöler, H., & Smith, A. (1998) · Cell · doi:10.1016/s0092-8674(00)81769-9 · Cited by 3,392
Self-citationFunctional Expression Cloning of Nanog, a Pluripotency Sustaining Factor in Embryonic Stem Cells
Chambers, I., Colby, D., Robertson, M., Nichols, J., Lee, S., Tweedie, S., & Smith, A. (2003) · Cell · doi:10.1016/s0092-8674(03)00392-1 · Cited by 3,255
Self-citationThe Homeoprotein Nanog Is Required for Maintenance of Pluripotency in Mouse Epiblast and ES Cells
Mitsui, K., Tokuzawa, Y., Itoh, H., Segawa, K., Murakami, M., Takahashi, K., Maruyama, M., Maeda, M., & Yamanaka, S. (2003) · Cell · doi:10.1016/s0092-8674(03)00393-3 · Cited by 3,191
Chen, X., Xu, H., Yuan, P., Fang, F., Huss, M., Vega, V. B., Wong, E., Orlov, Y. L., Zhang, W., Jiang, J., et al. (2008) · Cell · doi:10.1016/j.cell.2008.04.043 · Cited by 2,501
The Oct4 and Nanog transcription network regulates pluripotency in mouse embryonic stem cells
Loh, Y.-H., Wu, Q., Chew, J.-L., Vega, V. B., Zhang, W., Chen, X., Bourque, G., George, J., Leong, B., Liu, J., et al. (2006) · Nature Genetics · doi:10.1038/ng1760 · Cited by 2,437
Multipotent cell lineages in early mouse development depend on SOX2 function
Avilion, A. A., Nicolis, S. K., Pevny, L. H., Perez, L., Vivian, N., & Lovell-Badge, R. (2003) · Genes & Development · doi:10.1101/gad.224503 · Cited by 2,369
New cell lines from mouse epiblast share defining features with human embryonic stem cells
Tesar, P. J., Chenoweth, J. G., Brook, F. A., Davies, T. J., Evans, E. P., Mack, D. L., Gardner, R. L., & McKay, R. D. G. (2007) · Nature · doi:10.1038/nature05972 · Cited by 2,152
Huangfu, D., Maehr, R., Guo, W., Eijkelenboom, A., Snitow, M., Chen, A. E., & Melton, D. A. (2008) · Nature Biotechnology · doi:10.1038/nbt1418 · Cited by 1,606
Nanog safeguards pluripotency and mediates germline development
Chambers, I., Silva, J., Colby, D., Nichols, J., Nijmeijer, B., Robertson, M., Vrana, J., Jones, K., Grotewold, L., & Smith, A. (2007) · Nature · doi:10.1038/nature06403 · Cited by 1,500
Self-citationStem Cells, the Molecular Circuitry of Pluripotency and Nuclear Reprogramming
Jaenisch, R., & Young, R. (2008) · Cell · doi:10.1016/j.cell.2008.01.015 · Cited by 1,427
Connecting microRNA Genes to the Core Transcriptional Regulatory Circuitry of Embryonic Stem Cells
Marson, A., Levine, S. S., Cole, M. F., Frampton, G. M., Brambrink, T., Johnstone, S., Guenther, M. G., Johnston, W. K., Wernig, M., Newman, J., et al. (2008) · Cell · doi:10.1016/j.cell.2008.07.020 · Cited by 1,370
An Extended Transcriptional Network for Pluripotency of Embryonic Stem Cells
Kim, J., Chu, J., Shen, X., Wang, J., & Orkin, S. H. (2008) · Cell · doi:10.1016/j.cell.2008.02.039 · Cited by 1,332
Pluripotency governed by Sox2 via regulation of Oct3/4 expression in mouse embryonic stem cells
Masui, S., Nakatake, Y., Toyooka, Y., Shimosato, D., Yagi, R., Takahashi, K., Okochi, H., Okuda, A., Matoba, R., Sharov, A. A., et al. (2007) · Nature Cell Biology · doi:10.1038/ncb1589 · Cited by 1,252
A protein interaction network for pluripotency of embryonic stem cells
Wang, J., Rao, S., Chu, J., Shen, X., Levasseur, D. N., Theunissen, T. W., & Orkin, S. H. (2006) · Nature · doi:10.1038/nature05284 · Cited by 1,099
Nanog Is the Gateway to the Pluripotent Ground State
Silva, J., Nichols, J., Theunissen, T. W., Guo, G., van Oosten, A. L., Barrandon, O., Wray, J., Yamanaka, S., Chambers, I., & Smith, A. (2009) · Cell · doi:10.1016/j.cell.2009.07.039 · Cited by 1,031
Self-citationDissecting self-renewal in stem cells with RNA interference
Ivanova, N., Dobrin, R., Lu, R., Kotenko, I., Levorse, J., DeCoste, C., Schafer, X., Lun, Y., & Lemischka, I. R. (2006) · Nature · doi:10.1038/nature04915 · Cited by 959
A parallel circuit of LIF signalling pathways maintains pluripotency of mouse ES cells
Niwa, H., Ogawa, K., Shimosato, D., & Adachi, K. (2009) · Nature · doi:10.1038/nature08113 · Cited by 872
Niche-Independent Symmetrical Self-Renewal of a Mammalian Tissue Stem Cell
Conti, L., Pollard, S. M., Gorba, T., Reitano, E., Toselli, M., Biella, G., Sun, Y., Sanzone, S., Ying, Q.-L., Cattaneo, E., et al. (2005) · PLoS Biology · doi:10.1371/journal.pbio.0030283 · Cited by 870
Hanna, J., Cheng, A. W., Saha, K., Kim, J., Lengner, C. J., Soldner, F., Cassady, J. P., Muffat, J., Carey, B. W., & Jaenisch, R. (2010) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1004584107 · Cited by 847
Klf4 reverts developmentally programmed restriction of ground state pluripotency
Guo, G., Yang, J., Nichols, J., Hall, J. S., Eyres, I., Mansfield, W., & Smith, A. (2009) · Development · doi:10.1242/dev.030957 · Cited by 765
Hayashi, K., Lopes, S. M. C. D. S., Tang, F., & Surani, M. A. (2008) · Cell Stem Cell · doi:10.1016/j.stem.2008.07.027 · Cited by 649
Emerging properties of animal gene regulatory networks
Davidson, E. H. (2010) · Nature · doi:10.1038/nature09645 · Cited by 579
An Oct4-Centered Protein Interaction Network in Embryonic Stem Cells
van den Berg, D. L. C., Snoek, T., Mullin, N. P., Yates, A., Bezstarosti, K., Demmers, J., Chambers, I., & Poot, R. A. (2010) · Cell Stem Cell · doi:10.1016/j.stem.2010.02.014 · Cited by 542
Self-citationReprogramming of fibroblasts into induced pluripotent stem cells with orphan nuclear receptor Esrrb
Feng, B., Jiang, J., Kraus, P., Ng, J.-H., Heng, J.-C. D., Chan, Y.-S., Yaw, L.-P., Zhang, W., Loh, Y.-H., Han, J., et al. (2009) · Nature Cell Biology · doi:10.1038/ncb1827 · Cited by 470
The transcriptional foundation of pluripotency
Chambers, I., & Tomlinson, S. R. (2009) · Development · doi:10.1242/dev.024398 · Cited by 457
Self-citationOct4 and LIF/Stat3 Additively Induce Krüppel Factors to Sustain Embryonic Stem Cell Self-Renewal
Hall, J., Guo, G., Wray, J., Eyres, I., Nichols, J., Grotewold, L., Morfopoulou, S., Humphreys, P., Mansfield, W., Walker, R., et al. (2009) · Cell Stem Cell · doi:10.1016/j.stem.2009.11.003 · Cited by 383
Self-citationGlobal regulatory logic for specification of an embryonic cell lineage
Oliveri, P., Tu, Q., & Davidson, E. H. (2008) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0711220105 · Cited by 381
Greber, B., Wu, G., Bernemann, C., Joo, J. Y., Han, D. W., Ko, K., Tapia, N., Sabour, D., Sterneckert, J., Tesar, P., et al. (2010) · Cell Stem Cell · doi:10.1016/j.stem.2010.01.003 · Cited by 354
Nanog promotes transfer of pluripotency after cell fusion
Silva, J., Chambers, I., Pollard, S., & Smith, A. (2006) · Nature · doi:10.1038/nature04914 · Cited by 351
Self-citationMetastable Pluripotent States in NOD-Mouse-Derived ESCs
Hanna, J., Markoulaki, S., Mitalipova, M., Cheng, A. W., Cassady, J. P., Staerk, J., Carey, B. W., Lengner, C. J., Foreman, R., Love, J., et al. (2009) · Cell Stem Cell · doi:10.1016/j.stem.2009.04.015 · Cited by 328
Culture and differentiation of embryonic stem cells
Smith, A. G. (1991) · Journal of Tissue Culture Methods · doi:10.1007/bf01666137 · Cited by 265
Stat3 Activation Is Limiting for Reprogramming to Ground State Pluripotency
Yang, J., van Oosten, A. L., Theunissen, T. W., Guo, G., Silva, J. C. R., & Smith, A. (2010) · Cell Stem Cell · doi:10.1016/j.stem.2010.06.022 · Cited by 236
Epiblast Stem Cell Subpopulations Represent Mouse Embryos of Distinct Pregastrulation Stages
Han, D. W., Tapia, N., Joo, J. Y., Greber, B., Araúzo-Bravo, M. J., Bernemann, C., Ko, K., Wu, G., Stehling, M., Do, J. T., et al. (2010) · Cell · doi:10.1016/j.cell.2010.10.015 · Cited by 203
The developmental dismantling of pluripotency is reversed by ectopic Oct4 expression
Osorno, R., Tsakiridis, A., Wong, F., Cambray, N., Economou, C., Wilkie, R., Blin, G., Scotting, P. J., Chambers, I., & Wilson, V. (2012) · Development · doi:10.1242/dev.078071 · Cited by 171
Self-citationNanog Overcomes Reprogramming Barriers and Induces Pluripotency in Minimal Conditions
Theunissen, T. W., van Oosten, A. L., Castelo-Branco, G., Hall, J., Smith, A., & Silva, J. C. R. (2011) · Current Biology · doi:10.1016/j.cub.2010.11.074 · Cited by 161
Estrogen-Related Receptor Beta Interacts with Oct4 To Positively Regulate Nanog Gene Expression
van den Berg, D. L. C., Zhang, W., Yates, A., Engelen, E., Takacs, K., Bezstarosti, K., Demmers, J., Chambers, I., & Poot, R. A. (2008) · Molecular and Cellular Biology · doi:10.1128/mcb.00301-08 · Cited by 161
Self-citationZhang, X., Zhang, J., Wang, T., Esteban, M. A., & Pei, D. (2008) · Journal of Biological Chemistry · doi:10.1074/jbc.m803481200 · Cited by 158
Guo, G., & Smith, A. G. (2010) · Development · doi:10.1242/dev.052753 · Cited by 152
Chen, J., & Nathans, J. (2007) · Developmental Cell · doi:10.1016/j.devcel.2007.07.011 · Cited by 118
GeneProf: analysis of high-throughput sequencing experiments
Halbritter, F., Vaidya, H. J., & Tomlinson, S. R. (2011) · Nature Methods · doi:10.1038/nmeth.1809 · Cited by 93
Self-citationRobson, P., Stein, P., Zhou, B., Schultz, R. M., & Baldwin, H. S. (2001) · Developmental Biology · doi:10.1006/dbio.2001.0274 · Cited by 78
Vernallis, A. B., Hudson, K., & Heath, J. K. (1997) · Journal of Biological Chemistry · doi:10.1074/jbc.272.43.26947 · Cited by 52
Transcription factor heterogeneity and epiblast pluripotency
Osorno, R., & Chambers, I. (2011) · Philosophical Transactions of the Royal Society B Biological Sciences · doi:10.1098/rstb.2011.0043 · Cited by 38
Self-citationTranscription initiation: imposing specificity by localization
Ptashne, M., & Gann, A. A. F. (2001) · Essays in Biochemistry · doi:10.1042/bse0370001 · Cited by 25
Cited by
Master Transcription Factors and Mediator Establish Super-Enhancers at Key Cell Identity Genes
Whyte, W. A., Orlando, D. A., Hnisz, D., Abraham, B. J., Lin, C. Y., Kagey, M. H., Rahl, P. B., Lee, T. I., & Young, R. A. (2013) · Cell · doi:10.1016/j.cell.2013.03.035 · Cited by 4,143
Single Cell RNA-Sequencing of Pluripotent States Unlocks Modular Transcriptional Variation
Kolodziejczyk, A. A., Kim, J. K., Tsang, J. C. H., Ilicic, T., Henriksson, J., Natarajan, K. N., Tuck, A. C., Gao, X., Bühler, M., Liu, P., et al. (2015) · Cell Stem Cell · doi:10.1016/j.stem.2015.09.011 · Cited by 570
Dynamic stem cell states: Naive to primed pluripotency in rodents and humans
Weinberger, L., Ayyash, M., Novershtern, N., & Hanna, J. H. (2016) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm.2015.28 · Cited by 622
Epigenetics of Reprogramming to Induced Pluripotency
Papp, B., & Plath, K. (2013) · Cell · doi:10.1016/j.cell.2013.02.043 · Cited by 307
Mitotic binding of Esrrb marks key regulatory regions of the pluripotency network
Festuccia, N., Dubois, A., Vandormael-Pournin, S., Gallego Tejeda, E., Mouren, A., Bessonnard, S., Mueller, F., Proux, C., Cohen-Tannoudji, M., & Navarro, P. (2016) · Nature Cell Biology · doi:10.1038/ncb3418 · Cited by 158
Self-citation
Metadata
- Authors
- Festuccia, N., Osorno, R., Halbritter, F., Karwacki-Neisius, V., Navarro, P., Colby, D., Wong, F., Yates, A., Tomlinson, S. R., & Chambers, I.
- Venue
- Cell Stem Cell
- Publisher
- Elsevier BV
- Published
- 2012-10
- Volume
- 11
- Issue
- 4
- Pages
- 477-490
- ISSN
- 1875-9777 (ISSN-L)
- 1934-5909
- OpenAlex
- W2076612253
- Cited by
- 334
- References
- 48
- FWCI
- 13.24
- Type
- journal article
- Language
- en
- Open access
- hybrid
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
