Expression and subcellular distribution of native estrogen receptor β in murine C2C12 cells and skeletal muscle tissue
Open accessAbstract
We have recently described the expression and intracellular localization of ER alpha in murine C2C12 cells and skeletal muscle tissue. In separate studies, a protective role of 17beta-estradiol against apoptosis exerted mainly at the mitochondrial level was also shown in the C2C12 muscle cell line. However, this functional evidence was in accordance with the participation of ER beta. We have then here investigated the expression and subcellular distribution of native ER beta in similar skeletal muscle cultured cells and tissue developed in vivo. ER beta was detected by immunoblotting using specific antibodies and ligand blot analysis after subcellular fractionation. Immunolocalization was confirmed using conventional and confocal microscopy. ER beta was found to a great extent in mitochondria and in lower amounts in the cytosolic fraction, differently to ER alpha which localizes in microsomes, cytosol, mitochondria, and also in the nucleus of muscle tissue. ER beta expression was also demonstrated by RT-PCR. Finally, the mitochondrial localization of native ER beta in C2C12 muscle cells was corroborated after transient transfection with specific ER beta siRNAs. These data raise the possibility that the antiapoptotic action of 17beta-estradiol in muscle cells may be related in part to a direct action of the hormone on mitochondria through ER beta.
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Subpopulations of ERα and ERβ exist in mitochondria, although it is still unclear if they can directly regulate mtDNA transcription.
“Interestingly, it was found that subpopulations of ERα and ERβ exist in mitochondria, although it is still unclear if they can directly regulate mtDNA transcription (132–134).”
From From mitochondria to sarcopenia: role of 17β-estradiol and testosterone (2023) · cites this paper as [134]
Both ERα and ERβ are expressed in skeletal muscles of mice and humans.
“Both ERα and ERβ are expressed in skeletal muscles of mice and humans [10,12,13,14].”
From Phytoecdysteroids and Anabolic Effect of Atriplex dimorphostegia: UPLC-PDA-MS/MS Profiling, In Silico and In Vivo Models (2023) · cites this paper as [12]
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- Authors
- Milanesi, L., Vasconsuelo, A., de Boland, A. R., & Boland, R.
- Affiliations
- Universidad Nacional del Sur (AR)
- Corresponding author
- Boland, Ricardo
- ORCID
- Vasconsuelo, Andrea
- Venue
- Steroids
- Publisher
- Elsevier BV
- Published
- 2009-06
- Volume
- 74
- Issue
- 6
- Pages
- 489-497
- ISSN
- 0039-128X (ISSN-L)
- OpenAlex
- W1968488064
- Cited by
- 47
- References
- 33
- Citations by year
- 2026: 1 · 2024: 2 · 2023: 3 · 2021: 3 · 2020: 1 · 2019: 5 · 2018: 3 · 2017: 2 · 2016: 3 · 2015: 4 · 2014: 5 · 2013: 3 · 2012: 3
- FWCI
- 2.33
- Type
- journal article
- Language
- en
- Open access
- green
- hdl.handle.net
- Licence
- cc-by-nc-sa
- www.elsevier.com/tdm/userlicense/1.0/ (tdm)
- Locations
- Steroids (publishedVersion)
- PubMed (publishedVersion)
- CONICET Digital (CONICET) (publishedVersion, cc-by-nc-sa)
- Conicet (submittedVersion, cc-by-nc-sa)
- Funders
- Consejo Nacional de Investigaciones Científicas y Técnicas
- Agencia Nacional de Promoción Científica y Tecnológica (CONICET)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Estrogen and related hormone effects
- Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
- Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Effects and risks of endocrine disrupting chemicals
- Keywords
- estrogen receptor beta, subcellular localization, mitochondrial localization, C2C12 muscle cells, skeletal muscle, estradiol, apoptosis, anti-apoptotic effects, estrogen receptor alpha, siRNA knockdown, immunolocalization
- MeSH terms
- Animals, Cell Line, Cytosol, Estradiol, Immunohistochemistry, Mitochondria, Radioligand Assay, Subcellular Fractions, Transfection, Tritium, Blotting, Western, Gene Expression, Muscle, Skeletal, Microscopy, Confocal, Reverse Transcriptase Polymerase Chain Reaction, Myoblasts, RNA, Small Interfering, Estrogen Receptor beta, Mice
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