Phylogenetic classification of ten novel species belonging to the genus Bifidobacterium comprising B. phasiani sp. nov., B. pongonis sp. nov., B. saguinibicoloris sp. nov., B. colobi sp. nov., B. simiiventris sp. nov., B. santillanense sp. nov., B. miconis sp. nov., B. amazonense sp. nov., B. pluvialisilvae sp. nov., and B. miconisargentati sp. nov
Abstract
Ten Bifidobacterium strains, i.e., 6T3, 64T4, 79T10, 80T4, 81T8, 82T1, 82T10, 82T18, 82T24, and 82T25, were isolated from mantled guereza (Colobus guereza), Sumatran orangutan (Pongo abeli), silvery marmoset (Mico argentatus), golden lion tamarin (Leontopithecus rosalia), pied tamarin (Saguinus bicolor), and common pheasant (Phaisanus colchinus). Cells are Gram-positive, non-motile, non-sporulating, facultative anaerobic, and fructose 6-phosphate phosphoketolase-positive. Phylogenetic analyses based on the core genome sequences revealed that isolated strains exhibit close phylogenetic relatedness with Bifidobacterium genus members belonging to the Bifidobacterium bifidum, Bifidobacterium longum, Bifidobacterium pullorum, and Bifidobacterium tissieri phylogenetic groups. Phenotypic characterization and genotyping based on the genome sequences clearly show that these strains are distinct from each of the type strains of the so far recognized Bifidobacterium species. Thus, B. phasiani sp. nov. (6T3 = LMG 32224 T = DSM 112544 T), B. pongonis sp. nov. (64T4 = LMG 32281 T = DSM 112547 T), B. saguinibicoloris sp. nov. (79T10 = LMG 32232 T = DSM 112543 T), B. colobi sp. nov. (80T4 = LMG 32225 T = DSM 112552 T), B. simiiventris sp. nov. (81T8 = LMG 32226 T = DSM 112549 T), B. santillanense sp. nov. (82T1 = LMG 32284 T = DSM 112550 T), B. miconis sp. nov. (82T10 = LMG 32282 T = DSM 112551 T), B. amazonense sp. nov. (82T18 = LMG 32297 T = DSM 112548 T), pluvialisilvae sp. nov. (82T24 = LMG 32229 T = DSM 112545 T), and B. miconisargentati sp. nov. (82T25 = LMG 32283 T = DSM 112546 T) are proposed as novel Bifidobacterium species.
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The phylogenetically related B. bifidum species (PBS) are predominantly inhabitants of the non-human primates' gut.
“The phylogenetically related B. bifidum species (PBS), collectively forming the B. bifidum group, and Ninclude B. aerophilum, B. amazonense, B. biavatii, B. goeldii, B. hapali, B. jacchi, B. leontopitheci, B. miconis, B. ramosum, B. samirii, B. scardovii, and B. simiiventris , are predominantly inhabitants of the non-human primates' gut [25-28] .”
From Genome-scale insights into the metabolic landscape and evolutionary development of Bifidobacterium bifidum (2026) · cites this paper as [26]
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Selleri, E., Longhi, G., Tarracchini, C., Carolis, E. D., Petraro, S., Mancabelli, L., Milani, C., Turroni, F., Ventura, M., & Lugli, G. A. (2026) · Microbiome Research Reports · doi:10.20517/mrr.2026.19
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- Authors
- Lugli, G. A., Calvete-Torre, I., Alessandri, G., Milani, C., Turroni, F., Laiolo, P., Ossiprandi, M. C., Margolles, A., Ruiz, L., & Ventura, M.
- Affiliations
- University of Parma (IT)
- Universidad de Oviedo (ES)
- Corresponding author
- Ventura, Marco
- ORCID
- Venue
- Systematic and Applied Microbiology
- Publisher
- Elsevier BV
- Published
- 2021-11
- Volume
- 44
- Issue
- 6
- Pages
- 126273
- ISSN
- 0723-2020 (ISSN-L)
- OpenAlex
- W3207696291
- Cited by
- 21
- References
- 41
- Citations by year
- 2026: 4 · 2025: 1 · 2024: 6 · 2023: 4 · 2022: 5 · 2021: 1
- FWCI
- 3.61
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- Systematic and Applied Microbiology (publishedVersion)
- PubMed (publishedVersion)
- DIGITAL.CSIC (Spanish National Research Council (CSIC)) (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Phylogenetic Studies
- Physical Sciences › Environmental Science › Ecology › Microbial Community Ecology and Physiology
- Keywords
- Bifidobacterium, new species, Bifidobacterium bifidum, Bifidobacterium longum, core genome phylogeny, species delimitation, gut microbiota, primates, colobine monkeys
- MeSH terms
- Animals, Base Composition, Bifidobacterium, DNA, Bacterial, Fatty Acids, Feces, Phylogeny, RNA, Ribosomal, 16S, Bacterial Typing Techniques, Sequence Analysis, DNA
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