The DOI matches its Crossref record; 51 of the 51 references it cites were found in a registry or an index.
Flow Bioreactors as Complementary Tools for Biocatalytic Process Intensification
Open accessAbstract
Biocatalysis has widened its scope and relevance since new molecular tools, including improved expression systems for proteins, protein and metabolic engineering, and rational techniques for immobilization, have become available. However, applications are still sometimes hampered by low productivity and difficulties in scaling up. A practical and reasonable step to improve the performances of biocatalysts (including both enzymes and whole-cell systems) is to use them in flow reactors. This review describes the state of the art on the design and use of biocatalysis in flow reactors. The encouraging successes of this enabling technology are critically discussed, highlighting new opportunities, problems to be solved and technological advances.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Modifying enzyme activity and selectivity by immobilization
Rodrigues, R. C., Ortiz, C., Berenguer-Murcia, Á., Torres, R., & Fernández-Lafuente, R. (2013) · Chem. Soc. Rev. · doi:10.1039/c2cs35231a · Cited by 1,810
Cambié, D., Bottecchia, C., Straathof, N. J. W., Hessel, V., & Noël, T. (2016) · Chemical Reviews · doi:10.1021/acs.chemrev.5b00707 · Cited by 1,502
The past, present and potential for microfluidic reactor technology in chemical synthesis
Elvira, K. S., i Solvas, X. C., Wootton, R. C. R., & deMello, A. J. (2013) · Nature Chemistry · doi:10.1038/nchem.1753 · Cited by 1,203
On-demand continuous-flow production of pharmaceuticals in a compact, reconfigurable system
Adamo, A., Beingessner, R. L., Behnam, M., Chen, J., Jamison, T. F., Jensen, K. F., Monbaliu, J.-C. M., Myerson, A. S., Revalor, E. M., Snead, D. R., et al. (2016) · Science · doi:10.1126/science.aaf1337 · Cited by 941
Biocatalysis in Organic Chemistry and Biotechnology: Past, Present, and Future
Reetz, M. T. (2013) · Journal of the American Chemical Society · doi:10.1021/ja405051f · Cited by 762
Taming hazardous chemistry by continuous flow technology
Movsisyan, M., Delbeke, E. I. P., Berton, J. K. E. T., Battilocchio, C., Ley, S. V., & Stevens, C. V. (2016) · Chemical Society Reviews · doi:10.1039/c5cs00902b · Cited by 692
Evaluation of immobilized enzymes for industrial applications
Liese, A., & Hilterhaus, L. (2013) · Chemical Society Reviews · doi:10.1039/c3cs35511j · Cited by 673
Ten key issues in modern flow chemistry
Wegner, J., Ceylan, S., & Kirschning, A. (2011) · Chemical Communications · doi:10.1039/c0cc05060a · Cited by 641
Continuous flow reactors: a perspective
Wiles, C., & Watts, P. (2012) · Green Chem. · doi:10.1039/c1gc16022b · Cited by 626
Novel Process Windows for Enabling, Accelerating, and Uplifting Flow Chemistry
Hessel, V., Kralisch, D., Kockmann, N., Noël, T., & Wang, Q. (2013) · ChemSusChem · doi:10.1002/cssc.201200766 · Cited by 596
The role of flow in green chemistry and engineering
Newman, S. G., & Jensen, K. F. (2013) · Green Chemistry · doi:10.1039/c3gc40374b · Cited by 550
Flow chemistry—Microreaction technology comes of age
Jensen, K. F. (2017) · AIChE Journal · doi:10.1002/aic.15642 · Cited by 508
Organic Synthesis: March of the Machines
Ley, S. V., Fitzpatrick, D. E., Ingham, R. J., & Myers, R. M. (2015) · Angewandte Chemie International Edition · doi:10.1002/anie.201410744 · Cited by 455
Jiménez-González, C., Poechlauer, P., Broxterman, Q. B., Yang, B.-S., am Ende, D., Baird, J., Bertsch, C., Hannah, R. E., Dell’Orco, P., Noorman, H., et al. (2011) · Organic Process Research & Development · doi:10.1021/op100327d · Cited by 428
The Use of Gases in Flow Synthesis
Mallia, C. J., & Baxendale, I. R. (2016) · Organic Process Research & Development · doi:10.1021/acs.oprd.5b00222 · Cited by 361
The synthesis of active pharmaceutical ingredients (APIs) using continuous flow chemistry
Baumann, M., & Baxendale, I. R. (2015) · Beilstein Journal of Organic Chemistry · doi:10.3762/bjoc.11.134 · Cited by 359
Biocatalysis engineering: the big picture
Sheldon, R. A., & Pereira, P. C. (2017) · Chemical Society Reviews · doi:10.1039/c6cs00854b · Cited by 353
Advances in synthesis of biodiesel via enzyme catalysis: Novel and sustainable approaches
Guldhe, A., Singh, B., Mutanda, T., Permaul, K., & Bux, F. (2015) · Renewable and Sustainable Energy Reviews · doi:10.1016/j.rser.2014.09.035 · Cited by 275
Compartmentalized Droplets for Continuous Flow Liquid–Liquid Interface Catalysis
Zhang, M., Wei, L., Chen, H., Du, Z., Binks, B. P., & Yang, H. (2016) · Journal of the American Chemical Society · doi:10.1021/jacs.6b04265 · Cited by 238
KMnO4-Mediated Oxidation as a Continuous Flow Process
Sedelmeier, J., Ley, S. V., Baxendale, I. R., & Baumann, M. (2010) · Organic Letters · doi:10.1021/ol101345z · Cited by 217
Velasco‐Lozano, S., Benítez‐Mateos, A. I., & López‐Gallego, F. (2016) · Angewandte Chemie International Edition · doi:10.1002/anie.201609758 · Cited by 216
On Being Green: Can Flow Chemistry Help?
Ley, S. V. (2012) · The Chemical Record · doi:10.1002/tcr.201100041 · Cited by 207
Microreactors in Organic Chemistry and Catalysis
(2013) · Wiley eBooks · doi:10.1002/9783527659722 · Cited by 198
Continuous Flow Enzyme-Catalyzed Polymerization in a Microreactor
Kundu, S., Bhangale, A. S., Wallace, W. E., Flynn, K. M., Guttman, C. M., Gross, R. A., & Beers, K. L. (2011) · Journal of the American Chemical Society · doi:10.1021/ja111346c · Cited by 182
Enabling Technologies for the Future of Chemical Synthesis
Fitzpatrick, D. E., Battilocchio, C., & Ley, S. V. (2016) · ACS Central Science · doi:10.1021/acscentsci.6b00015 · Cited by 167
Biotransformations in microstructured reactors: more than flowing with the stream?
Bolivar, J. M., Wiesbauer, J., & Nidetzky, B. (2011) · Trends in biotechnology · doi:10.1016/j.tibtech.2011.03.005 · Cited by 144
Integration and application of optical chemical sensors in microbioreactors
Gruber, P., Marques, M. P. C., Szita, N., & Mayr, T. (2017) · Lab on a Chip · doi:10.1039/c7lc00538e · Cited by 142
Planchestainer, M., Contente, M. L., Cassidy, J., Molinari, F., Tamborini, L., & Paradisi, F. (2016) · Green Chemistry · doi:10.1039/c6gc01780k · Cited by 127
Self-citationA breakthrough method for the accurate addition of reagents in multi-step segmented flow processing
Lange, H., Carter, C. F., Hopkin, M. D., Burke, A., Goode, J. G., Baxendale, I. R., & Ley, S. V. (2011) · Chemical Science · doi:10.1039/c0sc00603c · Cited by 117
Rogers, T. A., & Bommarius, A. S. (2009) · Chemical Engineering Science · doi:10.1016/j.ces.2009.12.005 · Cited by 100
Towards a continuous flow environment for lipase-catalyzed reactions
Itabaiana, I., Miranda, L. S. M., & de Souza, R. O. M. A. (2012) · Journal of Molecular Catalysis B Enzymatic · doi:10.1016/j.molcatb.2012.08.008 · Cited by 83
Polakovič, M., Švitel, J., Bučko, M., Filip, J., Neděla, V., Ansorge‐Schumacher, M. B., & Gemeiner, P. (2017) · Biotechnology Letters · doi:10.1007/s10529-017-2300-y · Cited by 79
Toward Microfluidic Reactors for Cell‐Free Protein Synthesis at the Point‐of‐Care
Timm, A. C., Shankles, P. G., Foster, C. M., Doktycz, M. J., & Retterer, S. T. (2015) · Small · doi:10.1002/smll.201502764 · Cited by 68
Mesoscale Flow Chemistry: A Plug-Flow Approach to Reaction Optimisation
Wheeler, R. C., Benali, O., Deal, M. J., Farrant, E., Macdonald, S. J. F., & Warrington, B. H. (2007) · Organic Process Research & Development · doi:10.1021/op7000707 · Cited by 63
Bartha-Vári, J. H., Toșa, M. I., Irimie, F. D., Weiser, D., Boros, Z., Vértessy, B. G., Paizs, C., & Poppe, L. (2015) · ChemCatChem · doi:10.1002/cctc.201402894 · Cited by 57
Li, H., Moncecchi, J. R., & Truppo, M. D. (2015) · Organic Process Research & Development · doi:10.1021/op5003215 · Cited by 57
Dall’Oglio, F., Contente, M. L., Conti, P., Molinari, F., Danila, M., Pinto, A., Romano, D., Ubiali, D., Tamborini, L., & Serra, I. (2017) · Catalysis Communications · doi:10.1016/j.catcom.2017.01.025 · Cited by 51
Self-citationZambelli, P., Tamborini, L., Cazzamalli, S., Pinto, A., Arioli, S., Balzaretti, S., Plou, F. J., Fernández‐Arrojo, L., Molinari, F., Conti, P., et al. (2015) · Food Chemistry · doi:10.1016/j.foodchem.2015.06.002 · Cited by 49
Self-citationThree-Enzyme Cascade Bioreactor for Rapid Digestion of Genomic DNA into Single Nucleosides
Yin, J., Xu, T., Zhang, N., & Wang, H. (2016) · Analytical Chemistry · doi:10.1021/acs.analchem.6b01682 · Cited by 49
Valikhani, D., Bolivar, J. M., Pfeiffer, M., & Nidetzky, B. (2016) · ChemCatChem · doi:10.1002/cctc.201601019 · Cited by 48
Highly Efficient Oxidation of Amines to Aldehydes with Flow‐based Biocatalysis
Contente, M. L., Dall’Oglio, F., Tamborini, L., Molinari, F., & Paradisi, F. (2017) · ChemCatChem · doi:10.1002/cctc.201701147 · Cited by 45
Self-citationMembrane microreactors for catalytic reactions
Tan, X., & Li, K. (2013) · Journal of Chemical Technology & Biotechnology · doi:10.1002/jctb.4155 · Cited by 43
Enzymatic microreactors in biocatalysis: history, features, and future perspectives
Laurenti, E., & Vianna, A. D. S. (2016) · Biocatalysis · doi:10.1515/boca-2015-0008 · Cited by 43
Rapid protein immobilization for thin film continuous flow biocatalysis
Britton, J., Raston, C. L., & Weiss, G. A. (2016) · Chemical Communications · doi:10.1039/c6cc04210d · Cited by 41
Bolivar, J. M., Tribulato, M. A., Petrášek, Z., & Nidetzky, B. (2016) · Biotechnology and Bioengineering · doi:10.1002/bit.26011 · Cited by 38
Using flow technologies to direct the synthesis and assembly of materials in solution
Robertson, K. (2017) · Chemistry Central Journal · doi:10.1186/s13065-016-0229-1 · Cited by 22
Wiktor, P. J., Brunner, A., Kahn, P. A., Qiu, J., Magee, M., Bian, X., Karthikeyan, K., & LaBaer, J. (2015) · Scientific Reports · doi:10.1038/srep08736 · Cited by 20
Microreactors and CFD as Tools for Biocatalysis Reactor Design: A case study
Bodla, V. K., Seerup, R., Krühne, U., Woodley, J. M., & Gernaey, K. V. (2013) · Chemical Engineering & Technology · doi:10.1002/ceat.201200667 · Cited by 20
Stereoselective Hydrolase‐Catalyzed Processes in Continuous‐Flow Mode
Boros, Z., Hornyánszky, G., Nagy, J., et al. (2014) · doi:10.1002/9783527682492.ch9 · Cited by 9
Properties and Use of Microreactors
Barrow, D. A. (2008) · Wiley eBooks · doi:10.1002/9783527622856.ch3 · Cited by 6
Bioorganic and Biocatalytic Reactions
Miyazaki, M., Briones‐Nagata, M. P., Honda, T., & Yamaguchi, H. (2013) · Wiley eBooks · doi:10.1002/9783527659722.ch10 · Cited by 5
Cited by
Extending the application of biocatalysis to meet the challenges of drug development
Devine, P. N., Howard, R. M., Kumar, R., Thompson, M. P., Truppo, M. D., & Turner, N. J. (2018) · Nature Reviews Chemistry · doi:10.1038/s41570-018-0055-1 · Cited by 426
Britton, J., Majumdar, S., & Weiss, G. A. (2018) · Chemical Society Reviews · doi:10.1039/c7cs00906b · Cited by 389
The Hitchhiker's guide to biocatalysis: recent advances in the use of enzymes in organic synthesis
Sheldon, R. A., Brady, D., & Bode, M. L. (2020) · Chemical Science · doi:10.1039/c9sc05746c · Cited by 299
Biocatalysis with Laccases: An Updated Overview
Bassanini, I., Ferrandi, E. E., Riva, S., & Monti, D. (2020) · Catalysts · doi:10.3390/catal11010026 · Cited by 139
Wang, D., Zhang, H., Wang, Y., Pinelo, M., Mazzei, R., Fan, R., Wan, Y., & Luo, J. (2025) · Chemical Engineering Journal · doi:10.1016/j.cej.2025.160844 · Cited by 19
Metadata
- Authors
- Tamborini, L., Fernandes, P., Paradisi, F., & Molinari, F.
- Venue
- Trends in Biotechnology
- Publisher
- Elsevier BV
- Published
- 2018-01
- Volume
- 36
- Issue
- 1
- Pages
- 73-88
- ISSN
- 0167-7799 (ISSN-L)
- OpenAlex
- W2765697954
- Cited by
- 328
- References
- 51
- FWCI
- 2.37
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
