Gut microbiota modulates alcohol withdrawal-induced anxiety in mice
Open accessAbstract
Excessive alcohol consumption remains a major public health problem that affects millions of people worldwide. Accumulative experimental evidence has suggested an important involvement of gut microbiota in the modulation of host's immunological and neurological functions. However, it is previously unknown whether enteric microbiota is implicated in the formation of alcohol withdrawal-induced anxiety. Using a murine model of chronic alcoholism and withdrawal, we examined the impact of alcohol consumption on the possible alterations of gut microbiota as well as alcohol withdrawal-induced anxiety and behavior changes. The 16S rRNA sequencing revealed that alcohol consumption did not alter the abundance of bacteria, but markedly changed the composition of gut microbiota. Moreover, the transplantation of enteric microbes from alcohol-fed mice to normal healthy controls remarkably shaped the composition of gut bacteria, and elicited behavioral signs of alcohol withdrawal-induced anxiety. Using quantitative real-time polymerase chain reaction, we further confirmed that the expression of genes implicated in alcohol addiction, BDNF, CRHR1 and OPRM1, was also altered by transplantation of gut microbes from alcohol-exposed donors. Collectively, our findings suggested a possibility that the alterations of gut microbiota composition might contribute to the development of alcohol withdrawal-induced anxiety, and reveal potentially new etiologies for treating alcohol addiction.
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FMT from alcohol-treated mice to healthy control mice resulted in depressive behaviour in recipient mice.
“This transplantation resulted in depressive behaviour in the recipient mice. This behaviour was evaluated using the forced swim and tail suspension tests [29].”
From Effect of fecal microbiota transplant on symptoms of psychiatric disorders: a systematic review (2020) · cites this paper as [29]
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- Authors
- Xiao, H.-W., Ge, C., Feng, G.-X., Li, Y., Luo, D., Dong, J.-L., Li, H., Wang, H., Cui, M., & Fan, S.-J.
- Affiliations
- Chinese Academy of Medical Sciences & Peking Union Medical College (CN)
- Feinstein Institute for Medical Research (US)
- Corresponding author
- Cui, Ming
- Venue
- Toxicology Letters
- Publisher
- Elsevier BV
- Published
- 2018-05
- Volume
- 287
- Pages
- 23-30
- ISSN
- 0378-4274 (ISSN-L)
- PMC
- PMC5912338
- OpenAlex
- W2789550003
- Cited by
- 102
- References
- 36
- Citations by year
- 2026: 4 · 2025: 10 · 2024: 14 · 2023: 14 · 2022: 16 · 2021: 18 · 2020: 11 · 2019: 11 · 2018: 4
- FWCI
- 3.91
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Toxicology Letters (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Natural Science Foundation of China (81502664, 81572969, 81402541)
- CAMS Innovation Fund for Medical Sciences (2016-I2M-1-017)
- Fundamental Research Funds for CAMS/PUMC (2016ZX310200)
- PUMC Youth Fund and the Fundamental Research Funds for the Central Universities (33320140187, 3332016099, 3332016143)
- IRM-CAMS Research Fund (1547, 1522)
- Technology and Development and Research Projects for Research Institutes
- Ministry of Science and Technology (2014EG150134)
- Tianjin Science and Technology Support Plan Project (14ZCZDSY00001)
- U.S. National Center of Complementary and Alternative Medicine (R01AT005076)
- National Institute of General Medical Sciences (R01GM063075)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Health Sciences › Medicine › Gastroenterology › Gastrointestinal motility and disorders
- Health Sciences › Medicine › Pathology and Forensic Medicine › Alcohol Consumption and Health Effects
- Keywords
- gut microbiota, alcohol withdrawal, anxiety, alcoholism, chronic alcoholism, fecal microbiota transplantation, brain-derived neurotrophic factor, CRHR1, OPRM1, microbiota composition, mouse model
- MeSH terms
- Gastrointestinal Microbiome, Fecal Microbiota Transplantation, CRF Receptor, Type 1, Ethanol, Alcoholism, Animals, Anxiety, Bacteria, Behavior, Animal, Disease Models, Animal, Feces, Hippocampus, Intestines, Male, Mice, Inbred C57BL, Motor Activity, Substance Withdrawal Syndrome, Receptors, Opioid, mu, Receptors, Corticotropin-Releasing Hormone, Brain-Derived Neurotrophic Factor, Ribotyping, Host-Pathogen Interactions
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