HPLC methods for simultaneous determination of ascorbic and dehydroascorbic acids
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UV and ECD are the most common detection systems used in HPLC, but none of them enables the simultaneous detection of L-ascorbic and dehydroascorbic acid in a single run.
“UV (ultraviolet) and ECD (electrochemical detection) are the most common detection systems used in HPLC. Unfortunately, none of them enables the simultaneous detection of L-ascorbic and dehydroascorbic acid in a single run.”
From Vitamin C-Sources, Physiological Role, Kinetics, Deficiency, Use, Toxicity, and Determination (2021) · cites this paper as [256]
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- Authors
- Nováková, L., Solich, P., & Solichová, D.
- Affiliations
- Charles University (CZ)
- Corresponding author
- Nováková, L.
- Venue
- TrAC Trends in Analytical Chemistry
- Publisher
- Elsevier BV
- Published
- 2008-11
- Volume
- 27
- Issue
- 10
- Pages
- 942-958
- ISSN
- 0165-9936 (ISSN-L)
- OpenAlex
- W2027378012
- Cited by
- 210
- References
- 73
- Citations by year
- 2026: 6 · 2025: 17 · 2024: 15 · 2023: 13 · 2022: 10 · 2021: 12 · 2020: 16 · 2019: 15 · 2018: 7 · 2017: 16 · 2016: 17 · 2015: 9 · 2014: 13 · 2013: 10 · 2012: 13
- FWCI
- 6.36
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- TrAC Trends in Analytical Chemistry (publishedVersion)
- Topics
- Health Sciences › Nursing › Nutrition and Dietetics › Vitamin C and Antioxidants Research
- Physical Sciences › Chemistry › Spectroscopy › Analytical Chemistry and Chromatography
- Physical Sciences › Chemistry › Analytical Chemistry › Analytical Methods in Pharmaceuticals
- Keywords
- vitamin C, dehydroascorbic acid, simultaneous determination, HPLC assay, separation mechanism, detection methods, analyte stability, method validation
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