High-throughput analysis of vitamin C in human plasma with the use of HPLC with monolithic column and UV-detection
Abstract
Vitamin C plays a central role in the body. One of its important functions is its role as an antioxidant, and accurate measurements are important for interpretations of this role. However, its reactive nature and instability complicates the assessment, especially in biological samples. A high-throughput chromatographic method using monolithic column and UV-detection was developed for the assessment of plasma ascorbic acid and total ascorbic acid. The method showed excellent analytical sensitivity, specificity, precision, recovery and linearity during the validation study. The method was used for the assessment of ascorbic acid and total ascorbic acid during several clinical studies.
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Dehydroascorbic acid concentration in healthy adults is normally below 2% of that of L-ascorbic acid.
“Dehydroascorbic acid concentration in healthy adults is normally below 2% of that of L-ascorbic acid [211].”
From Vitamin C-Sources, Physiological Role, Kinetics, Deficiency, Use, Toxicity, and Determination (2021) · cites this paper as [211]
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- Authors
- KARLSEN, A., BLOMHOFF, R., & GUNDERSEN, T.
- Affiliations
- University of Oslo (NO)
- Corresponding author
- GUNDERSEN, T
- Venue
- Journal of Chromatography B
- Publisher
- Elsevier BV
- Published
- 2005-09-25
- Volume
- 824
- Issue
- 1-2
- Pages
- 132-138
- ISSN
- 1570-0232 (ISSN-L)
- OpenAlex
- W2077813312
- Cited by
- 94
- References
- 32
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 1 · 2023: 2 · 2022: 3 · 2021: 4 · 2020: 3 · 2019: 2 · 2018: 4 · 2017: 1 · 2016: 5 · 2015: 6 · 2014: 13 · 2013: 7 · 2012: 2
- FWCI
- 4.57
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Chromatography B (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Kreftforeningen
- Norges Forskningsråd
- Topics
- Health Sciences › Nursing › Nutrition and Dietetics › Vitamin C and Antioxidants Research
- Physical Sciences › Chemistry › Analytical Chemistry › Analytical Methods in Pharmaceuticals
- Physical Sciences › Chemistry › Spectroscopy › Analytical Chemistry and Chromatography
- Keywords
- vitamin C, plasma, total ascorbic acid, monoliths, high-throughput analysis, UV detection, antioxidants, biological sample analysis, method validation
- MeSH terms
- Adult, Ascorbic Acid, Chromatography, High Pressure Liquid, Dehydroascorbic Acid, Humans, Infant, Middle Aged, Reference Standards, Spectrophotometry, Ultraviolet, Reproducibility of Results
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