Cited by 94Vitamin C and Antioxidants ResearchAnalytical Methods in PharmaceuticalsAnalytical Chemistry and Chromatography

High-throughput analysis of vitamin C in human plasma with the use of HPLC with monolithic column and UV-detection

KARLSEN, A, BLOMHOFF, R, GUNDERSEN, T·2005·Journal of Chromatography B

Abstract

Vitamin C plays a central role in the body. One of its important functions is its role as an antioxidant, and accurate measurements are important for interpretations of this role. However, its reactive nature and instability complicates the assessment, especially in biological samples. A high-throughput chromatographic method using monolithic column and UV-detection was developed for the assessment of plasma ascorbic acid and total ascorbic acid. The method showed excellent analytical sensitivity, specificity, precision, recovery and linearity during the validation study. The method was used for the assessment of ascorbic acid and total ascorbic acid during several clinical studies.

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Crossref · 10.1016/j.jchromb.2005.07.008

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Authors
KARLSEN, A., BLOMHOFF, R., & GUNDERSEN, T.
Affiliations
University of Oslo (NO)
Corresponding author
GUNDERSEN, T
Venue
Journal of Chromatography B
Publisher
Elsevier BV
Published
2005-09-25
Volume
824
Issue
1-2
Pages
132-138
ISSN
1570-0232 (ISSN-L)
OpenAlex
W2077813312
Cited by
94
References
32
Citations by year
2026: 1 · 2025: 1 · 2024: 1 · 2023: 2 · 2022: 3 · 2021: 4 · 2020: 3 · 2019: 2 · 2018: 4 · 2017: 1 · 2016: 5 · 2015: 6 · 2014: 13 · 2013: 7 · 2012: 2
FWCI
4.57
Type
journal article
Language
en
Open access
closed
Locations
  • Journal of Chromatography B (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Kreftforeningen
  • Norges Forskningsråd
Topics
  • Health Sciences › Nursing › Nutrition and Dietetics › Vitamin C and Antioxidants Research
  • Physical Sciences › Chemistry › Analytical Chemistry › Analytical Methods in Pharmaceuticals
  • Physical Sciences › Chemistry › Spectroscopy › Analytical Chemistry and Chromatography
Keywords
vitamin C, plasma, total ascorbic acid, monoliths, high-throughput analysis, UV detection, antioxidants, biological sample analysis, method validation
MeSH terms
Adult, Ascorbic Acid, Chromatography, High Pressure Liquid, Dehydroascorbic Acid, Humans, Infant, Middle Aged, Reference Standards, Spectrophotometry, Ultraviolet, Reproducibility of Results

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