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Cited by 52Vector-borne infectious diseasesBartonella species infections researchInsect symbiosis and bacterial influences

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Molecular detection of Anaplasma platys, Ehrlichia canis, Hepatozoon canis and Rickettsia monacensis in dogs from Maio Island of Cape Verde archipelago

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Lauzi, Stefania, Maia, João P., Epis, Sara, Marcos, Ricardo, Pereira, Cristina, Luzzago, Camilla, Santos, Marta, Puente-Payo, Pablo, Giordano, Alessia, Pajoro, Massimo, Sironi, Giuseppe, Faustino, Augusto·2016·Ticks and Tick-borne Diseases
DOI 10.1016/j.ttbdis.2016.05.001
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Abstract

Tick-borne diseases are emerging worldwide and have an important zoonotic relevance. Dogs play an important role in the epidemiology of several zoonotic tick-borne pathogens acting as sentinels and/or reservoirs. This study focused on the molecular identification of tick-borne pathogens in blood samples of 153 autochthonous asymptomatic dogs in Maio Island, Cape Verde archipelago. Eighty-four (54.9%) dogs were positive for one or more pathogens. Fifty-five (35.9%) dogs were infected with Hepatozoon canis, 53 (34.6%) with Anaplasma platys, five (3.3%) with Ehrlichia canis and Rickettsia monacensis, an emerging human pathogen, was also identified in a single dog (0.7%). The former three pathogens cause important canine tick-borne diseases that are transmitted or potentially transmitted by Rhipicephalus sanguineus s.l., the only hard tick identified in Cape Verde. Furthermore, Wolbachia spp. was amplified from the blood of one dog. None of the dogs were positive for Anaplasma phagocytophilum, Borrelia burgdorferi sensu lato, Midichloria mitochondrii, Bartonella spp., Babesia spp. or Theileria spp. Fifty-four (35.3%) animals showed single infections and 30 (19.6%) co-infections, with A. platys and H. canis co-infection being the most frequent (28 dogs, 18.3%). The frequency of E. canis infection was statistically different among age groups (P=0.017), being higher among dogs older than 4 years compared to younger dogs. Infection by A. platys was also statistically different among age groups (P=0.031), being higher in dogs younger than 2 years compared to older dogs. The statistical analyses showed no significant association of PCR positivity with gender or location. The frequency of tick-borne pathogens detected in dogs in Maio Island, including R. monacensis, highlights the need to improve diagnosis and control in order to prevent the risk of transmission of these pathogens among dogs and humans living in or travelling to this touristic island.

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  • R. monacensis was identified in a blood sample of a dog (0.7%) in Maio Island, Cape Verde archipelago, but its pathogenicity in animals is still unknown.

    “R. monacensis was also identified in a blood sample of a dog (0.7%) in Maio Island, Cape Verde archipelago, however, its pathogenicity in animals is still unknown [26].”

    From Tick-borne pathogens in ticks collected from dogs, Latvia, 2011–2016 (2019) · cites this paper as [26]

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Research works printed but not linked: 50

  1. Aktas, 2015, Molecular detection of tick-borne rickettsial and protozoan pathogens in domestic dogs from Turkey, Parasites Vectors, 8, 157, 10.1186/s13071-015-0763-z · DOI
  2. Arraga-Alvarado, 2014, Case report: molecular evidence of Anaplasma platys infection in two women from Venezuela, Am. J. Trop. Med. Hyg., 91, 1161, 10.4269/ajtmh.14-0372 · DOI
  3. Baneth, 2011, Perspectives on canine and feline hepatozoonosis, Vet. Parasitol., 181, 3, 10.1016/j.vetpar.2011.04.015 · DOI
  4. Bazzocchi, 2003, Immunological role of the endosymbionts of Dirofilaria immitis: the Wolbachia surface protein activates canine neutrophils with production of IL-8, Vet. Parasitol., 117, 73, 10.1016/j.vetpar.2003.07.013 · DOI
  5. Bazzocchi, 2013, Molecular and serological evidence for the circulation of the tick symbiont Midichloria (Rickettsiales: midichloriaceae) in different mammalian species, Parasites Vectors, 6, 350, 10.1186/1756-3305-6-350 · DOI
  6. Beck, 2009, Diversity of Babesia and Theileria species in symptomatic and asymptomatic dogs in Croatia, Int. J. Parasitol., 39, 843, 10.1016/j.ijpara.2008.12.005 · DOI
  7. Benredjem, 2014, Borrelia garinii and Rickettsia monacensis in Ixodes ricinus ticks, Algeria, Emerg. Infect. Dis., 20, 1776, 10.3201/eid2010.140265 · DOI
  8. Chomel, 2006, Bartonella spp. in pets and effect on human health, Emerg. Infect. Dis., 12, 389, 10.3201/eid1203.050931 · DOI · Paper page
  9. Chomel, 2012, Candidatus Bartonella merieuxii, a potential new zoonotic Bartonella species in canids from Iraq, PLoS. Negl. Trop. Dis., 6, e1843, 10.1371/journal.pntd.0001843 · DOI
  10. Chomel, 2011, Tick-borne infections in dogs-an emerging infectious threat, Vet. Parasitol., 179, 294, 10.1016/j.vetpar.2011.03.040 · DOI
  11. Dantas-Torres, 2015, Further thoughts on the taxonomy and vector role of Rhipicephalus sanguineus group ticks, Vet. Parasitol., 208, 9, 10.1016/j.vetpar.2014.12.014 · DOI
  12. Dantas-Torres, 2012, Ticks and tick-borne diseases: a one health perspective, Trends Parasitol., 28, 437, 10.1016/j.pt.2012.07.003 · DOI · Paper page
  13. De Sousa, 2012, Role of the lizard Teira dugesii as a potential host for Ixodes ricinus tick-borne pathogens, Appl. Environ. Microbiol., 78, 3767, 10.1128/AEM.07945-11 · DOI
  14. Delord, 2014, Rickettsioses and Q fever in travelers (2004–2013), Travel Med. Infect. Dis., 12, 443, 10.1016/j.tmaid.2014.08.006 · DOI
  15. Duarte, 2013, The haematophagous arthropods (Animalia: Arthropoda) of the Cape Verde islands: a review, Zoologia Caboverdiana, 4, 31
  16. Epis, 2008, Midichloria mitochondrii is widespread in hard ticks (Ixodidae) and resides in the mitochondria of phylogenetically diverse species, Parasitology, 135, 485, 10.1017/S0031182007004052 · DOI
  17. Götsch, 2009, Ticks and haemoparasites of dogs from Praia, Cape Verde, Vet. Parasitol., 166, 171, 10.1016/j.vetpar.2009.08.009 · DOI
  18. Harrus, 1997, Clinical manifestations of infectious canine cyclic thrombocytopenia, Vet. Rec., 141, 247, 10.1136/vr.141.10.247 · DOI
  19. Harrus, 1998, Amplification of ehrlichial DNA from dogs 34 months after infection with Ehrlichia canis, J. Clin. Microbiol., 36, 73, 10.1128/JCM.36.1.73-76.1998 · DOI
  20. Hornok, 2013, Non-pet dogs as sentinels and potential synanthropic reservoirs of tick-borne and zoonotic bacteria, Vet. Microbiol., 167, 700, 10.1016/j.vetmic.2013.08.011 · DOI
  21. Inokuma, 2000, Detection of Ehrlichia platys DNA in brown dog ticks (Rhipicephalus sanguineus) in Okinawa Island, Japan, J. Clin. Microbiol., 38, 4219, 10.1128/JCM.38.11.4219-4221.2000 · DOI
  22. Jado, 2007, Rickettsia monacensis and human disease, Spain, Emerg. Infect. Dis., 13, 1405, 10.3201/eid1309.060186 · DOI
  23. Jensen, 2000, Rapid identification and differentiation of Bartonella species using a single-step PCR assay, J. Clin. Microbiol., 38, 1717, 10.1128/JCM.38.5.1717-1722.2000 · DOI
  24. Jovani, 2006, Parasite prevalence and sample size: misconceptions and solutions, Trends Parasitol., 22, 214, 10.1016/j.pt.2006.02.011 · DOI · Paper page
  25. Kilpatrick, 2012, Drivers, dynamics, and control of emerging vector-borne zoonotic diseases, Lancet, 380, 1946, 10.1016/S0140-6736(12)61151-9 · DOI
  26. Kirchner, 2008, Vector-borne parasites of dogs on the islands of Cabo Verde, Wien. Klin. Wochenschr., 120, 49, 10.1007/s00508-008-1075-5 · DOI
  27. Labruna, 2004, Rickettsia species infecting Amblyomma cooperi ticks from an area in the state of Sao Paulo, Brazil, where Brazilian spotted fever is endemic, J. Clin. Microbiol., 42, 90, 10.1128/JCM.42.1.90-98.2004 · DOI
  28. Landum, 2014, Detection of Wolbachia in Dirofilaria infected dogs in Portugal, Vet. Parasitol., 204, 407, 10.1016/j.vetpar.2014.05.027 · DOI · Paper page
  29. Leschnik, 2008, Imported non-endemic, arthropod-borne and parasitic infectious diseases in Austrian dogs, Wien. Klin. Wochenschr., 120, 59, 10.1007/s00508-008-1077-3 · DOI · Paper page
  30. Levin, 2012, Domestic dogs (Canis familiaris) as reservoir hosts for Rickettsia conorii, Vector Borne Zoonotic Dis., 12, 28, 10.1089/vbz.2011.0684 · DOI
  31. Madeddu, 2012, Rickettsia monacensis as cause of Mediterranean spotted fever-like illness, Italy, Emerg. Infect. Dis., 18, 702, 10.3201/eid1804.111583 · DOI
  32. Maia, 2015, Bacterial and protozoal agents of canine vector-borne diseases in the blood of domestic and stray dogs from southern Portugal, Parasites Vectors, 8, 138, 10.1186/s13071-015-0759-8 · DOI
  33. Marconi, 1992, Development of polymerase chain reaction primer sets for diagnosis of Lyme disease and for species-specific identification of Lyme disease isolates by 16S rRNA signature nucleotide analysis, J. Clin. Microbiol., 30, 2830, 10.1128/JCM.30.11.2830-2834.1992 · DOI
  34. Marcos, 2016, Buffy coat smear or Knott’s method: which to choose for microfilaria screening in field studies?, Vet. Clin. Pathol., 40, 201, 10.1111/vcp.12321 · DOI
  35. Massung, 1998, Nested PCR assay for detection of granulocytic ehrlichiae, J. Clin. Microbiol., 36, 1090, 10.1128/JCM.36.4.1090-1095.1998 · DOI
  36. Mi’ková, 2015, First detections of Rickettsia helvetica and R. monacensis in ectoparasitic mites (Laelapidae and Trombiculidae) infesting rodents in south-western Slovakia, Parasitol. Res., 114, 2465, 10.1007/s00436-015-4443-x · DOI
  37. Parola, 2000, Detection of ehrlichiae in African ticks by polymerase chain reaction, Trans. R. Soc. Trop. Med. Hyg., 94, 707, 10.1016/S0035-9203(00)90243-8 · DOI
  38. Parola, 2013, Update on tick-borne rickettsioses around the world: a geographic approach, Clin. Microbiol. Rev., 26, 657, 10.1128/CMR.00032-13 · DOI
  39. Pennisi, 2015, Ticks and associated pathogens collected from cats in Sicily and Calabria (Italy), Parasites Vectors, 8, 512, 10.1186/s13071-015-1128-3 · DOI
  40. Perez, 2006, Human infection with Ehrlichia canis accompanied by clinical signs in Venezuela, Ann. N. Y. Acad. Sci., 1078, 110, 10.1196/annals.1374.016 · DOI
  41. Roux, 1996, Differentiation of spotted fever group rickettsiae by sequencing and analysis of restriction fragment length polymorphism of PCR-amplified DNA of the gene encoding the protein rOmpA, J. Clin. Microbiol., 34, 2058, 10.1128/JCM.34.9.2058-2065.1996 · DOI
  42. Sainz, 2015, Guideline for veterinary practitioners on canine ehrlichiosis and anaplasmosis in Europe, Parasites Vectors, 8, 75, 10.1186/s13071-015-0649-0 · DOI · Paper page
  43. Schreiber, 2014, Pathogens in ticks collected from dogs in Berlin/Brandenburg, Germany, Parasites Vectors, 7, 535, 10.1186/s13071-014-0535-1 · DOI · Paper page
  44. Stich, 2002, Detection of Ehrlichia canis in canine carrier blood and in individual experimentally infected ticks with a p30-based PCR assay, J. Clin. Microbiol., 40, 540, 10.1128/JCM.40.2.540-546.2002 · DOI
  45. Sun, 2015, Detection of spotted fever group Rickettsiae in ticks from Zhejiang Province, China, Exp. Appl. Acarol., 65, 403, 10.1007/s10493-015-9880-9 · DOI
  46. Ujvari, 2004, High prevalence of Hepatozoon spp: (Apicomplexa: Hepatozoidae) infection in water pythons (Liasis fuscus) from tropical Australia, J. Parasitol., 90, 670, 10.1645/GE-204R · DOI
  47. Unver, 2003, Analysis of 16S rRNA gene sequences of Ehrlichia canis Anaplasma platys, and Wolbachia species from canine blood in Japan, Ann. N. Y. Acad. Sci., 990, 692, 10.1111/j.1749-6632.2003.tb07445.x · DOI
  48. Wächter, 2015, Serological differentiation of antibodies against Rickettsia helvetica, R., raoultii, R., slovaca, R. monacensis and R. felis in dogs from Germany by a micro-immunofluorescent antibody test, Parasites Vectors, 8, 126, 10.1186/s13071-015-0745-1 · DOI · Paper page
  49. Waner, 1997, Characterization of the subclinical phase of canine ehrlichiosis in experimentally infected beagle dogs, Vet. Parasitol., 69, 307, 10.1016/S0304-4017(96)01130-2 · DOI
  50. Ye, 2014, Vector competence of the tick Ixodes sinensis (Acari: Ixodidae) for Rickettsia monacensis, Parasites Vectors, 7, 512, 10.1186/s13071-014-0512-8 · DOI

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Metadata

Authors
Lauzi, S., Maia, J. P., Epis, S., Marcos, R., Pereira, C., Luzzago, C., Santos, M., Puente-Payo, P., Giordano, A., Pajoro, M., Sironi, G., & Faustino, A.
Affiliations
  • University of Milan (IT)
  • Universidade do Porto (PT)
Corresponding author
Lauzi, Stefania
ORCID
Venue
Ticks and Tick-borne Diseases
Publisher
Elsevier BV
Published
2016-07
Volume
7
Issue
5
Pages
964-969
ISSN
1877-959X (ISSN-L)
DOI
10.1016/j.ttbdis.2016.05.001
OpenAlex
W2345972079
Cited by
52
References
0
Citations by year
2026: 3 · 2025: 7 · 2024: 1 · 2023: 6 · 2022: 6 · 2021: 7 · 2020: 2 · 2019: 7 · 2018: 7 · 2017: 4 · 2016: 2
FWCI
5.41
Type
journal article
Language
en
Open access
Licence
Locations
  • Ticks and Tick-borne Diseases (publishedVersion)
  • PubMed (publishedVersion)
  • Archivio Istituzionale della Ricerca (Universita Degli Studi Di Milano) (submittedVersion, other-oa)
Funders
University of Milan, Italy
Topics
  • Life Sciences › Immunology and Microbiology › Parasitology › Vector-borne infectious diseases
  • Health Sciences › Medicine › Infectious Diseases › Bartonella species infections research
  • Life Sciences › Agricultural and Biological Sciences › Insect Science › Insect symbiosis and bacterial influences
Keywords
Anaplasma platys, Ehrlichia canis, Hepatozoon canis, Rickettsia monacensis, canine tick-borne diseases, dogs, Rhipicephalus sanguineus, co-infection, asymptomatic infection, Wolbachia, zoonotic pathogens, Maio Island, Cape Verde, age-related infection risk
MeSH terms
Anaplasma, Anaplasmosis, Animals, Coccidiosis, Dog Diseases, Dogs, Female, Humans, Male, Rickettsia, Rickettsia Infections, Eucoccidiida, Ehrlichiosis, Tick-Borne Diseases, Wolbachia, Ehrlichia canis, Cabo Verde, Coinfection

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