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Cited by 82Probiotics and Fermented FoodsAnimal Nutrition and PhysiologyReproductive tract infections research

Isolation of surface (S) layer protein carrying Lactobacillus species from porcine intestine and faeces and characterization of their adhesion properties to different host tissues

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Jakava-Viljanen, Miia, Palva, Airi·2007·Veterinary Microbiology
DOI 10.1016/j.vetmic.2007.04.029
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Abstract

Surface-layer proteins (Slps) of lactobacilli have been shown to confer tissue adherence. This study aimed to isolate and identify Slps carrying Lactobacillus species from the porcine intestine and faeces and to characterize these S-layer-expressing strains for their ability to adhere to the pig and human intestinal cells and to extracellular matrix (ECM) proteins. In total 99 strains, putatively belonging to the genus Lactobacillus, were isolated as pure cultures. SDS-PAGE and a gene probe specific for the Lactobacillus brevis ATCC 8287 S-layer protein gene (slpA) were used to screen the presence of strains possessing putative Slps. Eight of the 99 pure cultures exhibited Slps according to the SDS-PAGE analyses. In these strains the presence of genes encoding Slps was confirmed by PCR and partial sequencing. Only one isolate of the 99 strains gave a positive hybridisation signal with the L. brevis slpA probe but did not appear to produce S-layer protein. Their taxonomic identification, based on phenotyping and the 16S rRNA sequences, revealed that the eight S-layer protein-producing strains were closely related to Lactobacillus amylovorus, Lactobacillus sobrius and Lactobacillus crispatus. The strain with the slpA positive hybridisation result was identified as Lactobacillus mucosae. The SDS-extractable protein profile, the size of the putative S-layer protein and binding capability of the strains varied greatly, even among the isolates belonging to the same Lactobacillus cluster. Removal of the intact Slps from the bacterial surface by extraction with guanidine hydrochloride reduced the adhesion of some strains to fibronectin and laminin, whereas, the adhesiveness to laminin increased with some strains.

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  • Besides surface layer proteins, other surface structures such as lipoteichoic acids and pili, passive forces, electrostatic interactions, and hydrophobicity affect probiotics' adherence to the host.

    “The literature acknowledges that besides surface layer proteins, other surface structures such as lipoteichoic acids and pili, passive forces, electrostatic interactions, and hydrophobicity affects the probiotics' adherence to the host [28,50].”

    From The Biological Role of the S-Layer Produced by Lactobacillus helveticus 34.9 in Cell Protection and Its Probiotic Properties (2024) · cites this paper as [50]

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Authors
Jakava-Viljanen, M., & Palva, A.
Affiliations
University of Helsinki (FI)
Corresponding author
Jakava-Viljanen, Miia
Venue
Veterinary Microbiology
Publisher
Elsevier BV
Published
2007-10
Volume
124
Issue
3-4
Pages
264-273
ISSN
0378-1135 (ISSN-L)
DOI
10.1016/j.vetmic.2007.04.029
OpenAlex
W2085512170
Cited by
82
References
32
Citations by year
2026: 2 · 2025: 1 · 2024: 1 · 2023: 3 · 2022: 1 · 2021: 3 · 2020: 2 · 2019: 6 · 2018: 8 · 2017: 5 · 2016: 5 · 2015: 3 · 2014: 7 · 2013: 5 · 2012: 8
FWCI
7.58
Type
journal article
Language
en
Open access
Licence
Locations
  • Veterinary Microbiology (publishedVersion)
  • PubMed (publishedVersion)
  • HAL (submittedVersion, other-oa)
  • Trinity's Access to Research Output (TARA) (Trinity College Dublin) (submittedVersion)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
  • Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Nutrition and Physiology
  • Life Sciences › Immunology and Microbiology › Microbiology › Reproductive tract infections research
Keywords
surface layer protein, S-layer, Lactobacillus, porcine intestine, pig feces, bacterial adhesion, extracellular matrix proteins, fibronectin, laminin, Lactobacillus amylovorus, Lactobacillus crispatus, Lactobacillus mucosae, guanidine hydrochloride extraction, pigs
MeSH terms
Amino Acid Sequence, Animals, Bacterial Adhesion, Bacterial Proteins, DNA, Bacterial, DNA, Ribosomal, Electrophoresis, Polyacrylamide Gel, Feces, Humans, Intestines, Lactobacillus, Molecular Sequence Data, Phylogeny, RNA, Bacterial, RNA, Ribosomal, 16S, Swine, Polymerase Chain Reaction, Extracellular Matrix Proteins, Sequence Alignment

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