Activity of Syzygium aromaticum essential oil and its main constituent eugenol in the inhibition of the development of Ctenocephalides felis felis and the control of adults
Abstract
The aim of this study was to evaluate the in vitro activity of Syzygium aromaticum essential oil (SAEO) and its main constituent eugenol (EG) against adult fleas and their action in the maturation of eggs into adults of Ctenocephalides felis felis. In order to evaluate the pulicidal activity, 10 adult fleas were exposed to a filter paper impregnated with SAEO and EG at increasing concentrations of 0.047; 0.094; 0.188; 0.375; 0.750; 1.50; 3.00; 6.00; 12.00 and 24.00 μg cm-2. Flea mortality was evaluated 24 and 48 h after exposure. In order to evaluate the inhibition of the maturation of eggs into adults, 10 eggs were exposed to filter paper impregnated with SAEO and EG at the same concentrations used in the pulicidal test, and the evaluation was performed 30 days after incubation. Untreated repetitions were maintained in both studies (control group). The lethal concentration 50 (LC 50) of pulicidal activity to SAEO was 5.70 μg cm-2 in 24 h and 3.91 μg cm-2 in 48 h. The LC 90 was 16.10 μg cm-2 and 15.80 μg cm-2 in 24 and 48 h, respectively. The LC 50 of inhibition of the maturation of eggs into adults was 0.30 μg cm-2 and the LC 90 3.44 μg cm-2. The LC 50 of pulicidal activity to EG was 2.40 μg cm-2 in 24 h and 1.40 μg cm-2 in 48 h; the LC 90 was 8.10 μg cm-2 and 3.70 μg cm-2 in 24 h and 48 h, respectively. The LC 50 of inhibition of the maturation of eggs into adults was 0.10 μg cm-2 and the LC 90 0.68 μg cm-2. Based on the results obtained, it is possible to conclude that the both SAEO and EG have in vitro pulicidal activity as well as acting as inhibitors of the maturation of eggs into adults of the flea C. felis felis.
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Lambert et al. evaluated the activity of CEO against adult C. felis felis and the development of their eggs; the LC50 was 5.70 μg/cm2 against adult fleas and 0.30 g/cm2 against flea eggs; however, the insecticidal activity of eugenol was three times higher.
“Lambert et al. [103] evaluated the activity of CEO against adult C. felis felis and the development of their eggs. The LC50 was 5.70 μg/cm2 against adult fleas and 0.30 g/cm2 against flea eggs; however, the insecticidal activity of eugenol was three times higher [103].”
From Clove essential oil (Syzygium aromaticum L. Myrtaceae): Extraction, chemical composition, food applications, and essential bioactivity for human health (2021) · cites this paper as [103]
CEO has shown high levels of repellency and fumigant toxicity on flea, aphids, nymphal instars, mites, imported red fire ants, C. pipiens, and American and German cockroaches.
“CEO has shown high levels of repellency and fumigant toxicity on flea, aphids, nymphal instars, mites, imported red fire ants, C. pipiens, and American and German cockroaches [103,105,106].”
From Clove essential oil (Syzygium aromaticum L. Myrtaceae): Extraction, chemical composition, food applications, and essential bioactivity for human health (2021) · cites this paper as [103]
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- Authors
- Lambert, M. M., Campos, D. R., Borges, D. A., de Avelar, B. R., Ferreira, T. P., Cid, Y. P., Boylan, F., Scott, F. B., de Almeida Chaves, D. S., & Coumendouros, K.
- Affiliations
- Universidade Federal Rural do Rio de Janeiro (BR)
- Trinity College Dublin (IE)
- Corresponding author
- Campos, Diefrey Ribeiro
- ORCID
- Venue
- Veterinary Parasitology
- Publisher
- Elsevier BV
- Published
- 2020-06
- Volume
- 282
- Pages
- 109126
- ISSN
- 0304-4017 (ISSN-L)
- OpenAlex
- W3025022465
- Cited by
- 35
- References
- 42
- Citations by year
- 2026: 5 · 2025: 2 · 2024: 6 · 2023: 4 · 2022: 11 · 2021: 4 · 2020: 3
- FWCI
- 3.55
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Veterinary Parasitology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Fundação de Apoio à Pesquisa Tecnológica da Universidade Federal Rural do Rio de Janeiro
- Coordenação de Aperfeiçoamento Pessoal de Nível Superior
- Conselho Nacional de Desenvolvimento Científico e Tecnológico
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Insect Pest Control Strategies
- Life Sciences › Agricultural and Biological Sciences › Food Science › Essential Oils and Antimicrobial Activity
- Health Sciences › Medicine › Infectious Diseases › Dermatological diseases and infestations
- Keywords
- Syzygium aromaticum, eugenol, essential oils, Ctenocephalides felis felis, Ctenocephalides felis, botanical insecticides, LC50, LC90
- MeSH terms
- Animals, Eugenol, Female, Insect Control, Insecticides, Male, Oils, Volatile, Syzygium, Ctenocephalides
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