Cited by 87HIV Research and TreatmentHIV-related health complications and treatmentsAdvanced Proteomics Techniques and Applications

Investigating the human immunodeficiency virus type 1-infected monocyte-derived macrophage secretome

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Ciborowski, Pawel, Kadiu, Irena, Rozek, Wojciech, Smith, Lynette, Bernhardt, Kristen, Fladseth, Melissa, Ricardo-Dukelow, Mary, Gendelman, Howard E.·2007·Virology

Abstract

Mononuclear phagocytes (bone marrow monocyte-derived macrophages, alveolar macrophages, perivascular macrophages, and microglia) are reservoirs and vehicles of dissemination for the human immunodeficiency virus type-1 (HIV-1). How virus alters mononuclear phagocyte immunoregulatory activities to complete its life cycle and influence disease is incompletely understood. In attempts to better understanding the influence of virus on macrophage functions, we used one-dimensional electrophoresis, and liquid chromatography tandem mass spectrometry to analyze the secretome of HIV-1-infected human monocyte-derived macrophages. We identified 110 proteins in culture supernatants of control (uninfected) and virus-infected cells. Differentially expressed cytoskeletal, enzymes, redox, and immunoregulatory protein classes were discovered and validated by Western blot tests. These included, but were not limited to, cystatin C, cystatin B, chitinase 3-like 1 protein, cofilin-1, l-plastin, superoxide dismutase, leukotriene A(4) hydrolase, and alpha-enolase. This study, using a unique proteomics platform, provides novel insights into virus-host cell interactions that likely affect the functional role of macrophages in HIV disease.

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Crossref · 10.1016/j.virol.2007.01.013

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  1. Gendelman, H.E., Morahan, P.M., 1992. The macrophage in viral infections. In: Lewis, C.E., McGee, J. (Eds.), The Natural Immune System Series: The Macrophage. Oxford Univ. Press, London, pp. 156-232.

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Authors
Ciborowski, P., Kadiu, I., Rozek, W., Smith, L., Bernhardt, K., Fladseth, M., Ricardo-Dukelow, M., & Gendelman, H. E.
Affiliations
University of Nebraska Medical Center (US)
Corresponding author
Ciborowski, Pawel
Venue
Virology
Publisher
Elsevier BV
Published
2007-06
Volume
363
Issue
1
Pages
198-209
ISSN
0042-6822 (ISSN-L)
OpenAlex
W1990974472
Cited by
87
References
54
Citations by year
2024: 4 · 2022: 5 · 2021: 3 · 2020: 2 · 2019: 2 · 2018: 1 · 2017: 4 · 2016: 2 · 2015: 6 · 2014: 7 · 2013: 4 · 2012: 9
FWCI
3.91
Type
journal article
Language
en
Open access
Locations
  • Virology (publishedVersion)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
Funders
  • National Institutes of Health (5r21mh075662-02, R37 NS36126, 5 R37 NS36126-07)
  • National Institute of Mental Health (1 R21 MH075662-01, P01MH064570, 5 R01 MH64570-03, R21 MH075662)
  • National Institute of Neurological Disorders and Stroke (5 P01NS31492-11, 5 R37 NS36126-07, R37NS036126, P01NS011766, P01NS031492, 1P01 NS43985-01A1, P01 NS11766-28, P01NS043985, R01NS034239)
  • National Center for Research Resources (P20RR015635, 20 RR15635)
Topics
  • Life Sciences › Immunology and Microbiology › Virology › HIV Research and Treatment
  • Health Sciences › Medicine › Emergency Medicine › HIV-related health complications and treatments
  • Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
Keywords
HIV-1, monocyte-derived macrophages, secretome, host-virus interaction, mononuclear phagocytes, cystatin C, cystatin B, YKL-40, cofilin-1, L-plastin, superoxide dismutase, leukotriene A4 hydrolase, alpha-enolase, redox proteins, cytoskeletal proteins, HIV reservoir
MeSH terms
Cells, Cultured, Electrophoresis, Polyacrylamide Gel, Gene Expression Regulation, Humans, Macrophages, Monocytes, Proteins, Time Factors, Blotting, Western, HIV-1, Spectrometry, Mass, Electrospray Ionization

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