Investigating the human immunodeficiency virus type 1-infected monocyte-derived macrophage secretome
Open accessAbstract
Mononuclear phagocytes (bone marrow monocyte-derived macrophages, alveolar macrophages, perivascular macrophages, and microglia) are reservoirs and vehicles of dissemination for the human immunodeficiency virus type-1 (HIV-1). How virus alters mononuclear phagocyte immunoregulatory activities to complete its life cycle and influence disease is incompletely understood. In attempts to better understanding the influence of virus on macrophage functions, we used one-dimensional electrophoresis, and liquid chromatography tandem mass spectrometry to analyze the secretome of HIV-1-infected human monocyte-derived macrophages. We identified 110 proteins in culture supernatants of control (uninfected) and virus-infected cells. Differentially expressed cytoskeletal, enzymes, redox, and immunoregulatory protein classes were discovered and validated by Western blot tests. These included, but were not limited to, cystatin C, cystatin B, chitinase 3-like 1 protein, cofilin-1, l-plastin, superoxide dismutase, leukotriene A(4) hydrolase, and alpha-enolase. This study, using a unique proteomics platform, provides novel insights into virus-host cell interactions that likely affect the functional role of macrophages in HIV disease.
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Cites
Anderson, N. L., & Anderson, N. G. (2002) · Molecular & Cellular Proteomics · doi:10.1074/mcp.r200007-mcp200 · Cited by 4,414 · see citing works
Prostaglandins and Leukotrienes: Advances in Eicosanoid Biology
Funk, C. D. (2001) · Science · doi:10.1126/science.294.5548.1871 · Cited by 3,817 · see citing works
The Role of Mononuclear Phagocytes in HTLV-III/LAV Infection
Gartner, S., Markovits, P., Markovitz, D. M., Kaplan, M. H., Gallo, R. C., & Popovič, M. (1986) · Science · doi:10.1126/science.3014648 · Cited by 1,808 · see citing works
Dahlén, S., Björk, J., Hedqvist, P., Arfors, K. E., Hammarström, S., Lindgren, J. A., & Samuelsson, B. O. (1981) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.78.6.3887 · Cited by 1,019 · see citing works
Gendelman, H. E., Orenstein, J. M., Martin, M. A., Ferrua, C., Mitra, R., Phipps, T. J., Wahl, L. A., Lane, H. C., Fauci, A. S., & Burke, D. (1988) · The Journal of Experimental Medicine · doi:10.1084/jem.167.4.1428 · Cited by 781 · see citing works
Self-citationSecretion of neurotoxins by mononuclear phagocytes infected with HIV-1
Giulian, D., Vaca, K., & Noonan, C. (1990) · Science · doi:10.1126/science.2148832 · Cited by 651 · see citing works
Production of superoxide anions by a CNS macrophage, the microglia
Colton, C. A., & Gilbert, D. L. (1987) · FEBS Letters · doi:10.1016/0014-5793(87)80305-8 · Cited by 614 · see citing works
Neurological complications of HIV infection
McArthur, J. C., Brew, B. J., & Nath, A. (2005) · The Lancet Neurology · doi:10.1016/s1474-4422(05)70165-4 · Cited by 557 · see citing works
Vimentin is secreted by activated macrophages
Mor-Vaknin, N., Punturieri, A., Sitwala, K., & Markovitz, D. M. (2003) · Nature Cell Biology · doi:10.1038/ncb898 · Cited by 522 · see citing works
A panel of cerebrospinal fluid potential biomarkers for the diagnosis of Alzheimer's disease
Carrette, O., Demalte, I., Scherl, A., Yalkinoglu, O., Corthals, G. L., Burkhard, P. R., Hochstrasser, D. F., & Sanchez, J. (2003) · PROTEOMICS · doi:10.1002/pmic.200300470 · Cited by 375 · see citing works
The macrophage in the persistence and pathogenesis of HIV infection
Gendelman, H. E., Orenstein, J. M., Baca, L. M., et al. (1989) · AIDS · doi:10.1097/00002030-198908000-00001 · Cited by 356 · see citing works
Self-citationHIV-1 infection and AIDS: consequences for the central nervous system
Kaul, M., Zheng, J., Okamoto, S., Gendelman, H. E., & Lipton, S. A. (2005) · Cell Death and Differentiation · doi:10.1038/sj.cdd.4401623 · Cited by 344 · see citing works
Self-citationEvidence That HIV Budding in Primary Macrophages Occurs through the Exosome Release Pathway
Nguyen, D. G., Booth, A. M., Gould, S. J., & Hildreth, J. E. K. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m309009200 · Cited by 337 · see citing works
Cells of the central nervous system as targets and reservoirs of the human immunodeficiency virus
Kramer-Hämmerle, S., Rothenaigner, I., Wolff, H., Bell, J. E., & Brack‐Werner, R. (2005) · Virus Research · doi:10.1016/j.virusres.2005.04.009 · Cited by 335 · see citing works
Platelet-activating factor: a candidate human immunodeficiency virus type 1-induced neurotoxin
Gelbard, H. A., Nottet, H. S. L. M., Swindells, S., Jett, M., Dzenko, K. A., Genis, P., White, R., Wang, L., Choi, Y., & Zhang, D. (1994) · Journal of Virology · doi:10.1128/jvi.68.7.4628-4635.1994 · Cited by 318 · see citing works
Goetzl, E. J., An, S., & Smith, W. L. (1995) · The FASEB Journal · doi:10.1096/fasebj.9.11.7649404 · Cited by 275 · see citing works
Macrophage defences against respiratory tract infections
Gordon, S. B., & Read, R. C. (2002) · British Medical Bulletin · doi:10.1093/bmb/61.1.45 · Cited by 248 · see citing works
Zhou, Y., Kurihara, T., Ryseck, R.-P., Yang, Y., Ryan, C. S., Loy, J. K., Warr, G. A., & Bravo, R. (1998) · The Journal of Immunology · doi:10.4049/jimmunol.160.8.4018 · Cited by 245 · see citing works
Tonnesen, M. G., Anderson, D. C., Springer, T. A., Knedler, A., Avdi, N., & Henson, P. M. (1989) · Journal of Clinical Investigation · doi:10.1172/jci113928 · Cited by 236 · see citing works
Cu/Zn Superoxide Dismutase Plays Important Role in Immune Response
Marikovsky, M., Ziv, V., Nevo, N., Harris‐Cerruti, C., & Mahler, O. S. (2003) · The Journal of Immunology · doi:10.4049/jimmunol.170.6.2993 · Cited by 206 · see citing works
The Role of Monocytes and Macrophages in the Pathogenesis of HIV-1 Infection
Kedzierska, K., & Crowe, S. M. (2002) · Current Medicinal Chemistry · doi:10.2174/0929867023368935 · Cited by 198 · see citing works
Giulian, D., Corpuz, M., Chapman, S., Mansouri, M. M., & Robertson, C. S. (1993) · Journal of Neuroscience Research · doi:10.1002/jnr.490360609 · Cited by 191 · see citing works
Enhanced HIV replication in macrophage colony-stimulating factor-treated monocytes
Kalter, D. C., Nakamura, M., Turpin, J. A., et al. (1991) · The Journal of Immunology · doi:10.4049/jimmunol.146.1.298 · Cited by 140 · see citing works
Potential roles of cellular proteins in HIV‐1
Ott, D. E. (2002) · Reviews in Medical Virology · doi:10.1002/rmv.367 · Cited by 116 · see citing works
Cystatin C as a potential cerebrospinal fluid marker for the diagnosis of Creutzfeldt‐Jakob disease
Sanchez, J., Guillaume, E., Lescuyer, P., et al. (2004) · PROTEOMICS · doi:10.1002/pmic.200300799 · Cited by 109 · see citing works
Vázquez, N., Greenwell‐Wild, T., Marinos, N. J., Swaim, W. D., Nares, S., Ott, D. E., Schubert, U., Henklein, P., Orenstein, J. M., Sporn, M. B., et al. (2005) · Journal of Virology · doi:10.1128/jvi.79.7.4479-4491.2005 · Cited by 102 · see citing works
Cystatin C and cathepsin B in CSF from patients with inflammatory neurologic diseases
Nagai, A., Murakawa, Y., Terashima, M., Shimode, K., Umegae, N., Takeuchi, H., & Kobayashi, S. (2000) · Neurology · doi:10.1212/wnl.55.12.1828 · Cited by 101 · see citing works
Cleavage of cystatin C in the cerebrospinal fluid of patients with multiple sclerosis
Irani, D. N., Anderson, C., Gundry, R. L., et al. (2006) · Annals of Neurology · doi:10.1002/ana.20786 · Cited by 98 · see citing works
Ichikawa, M., Sugita, M., Takahashi, M., et al. (2003) · Immunology · doi:10.1046/j.1365-2567.2003.01572.x · Cited by 97 · see citing works
Regulation of leukocyte adhesion and signaling in inflammation and disease
Wahl, S. M., Feldman, G. M., & McCarthy, J. B. (1996) · Journal of Leukocyte Biology · doi:10.1002/jlb.59.6.789 · Cited by 88 · see citing works
Ghorpade, A., Persidskaia, R., Suryadevara, R., Che, M., Liu, X. J., Persidsky, Y., & Gendelman, H. E. (2001) · Journal of Virology · doi:10.1128/jvi.75.14.6572-6583.2001 · Cited by 86 · see citing works
Self-citationMeltzer, M. S., & Gendelman, H. E. (1992) · Current topics in microbiology and immunology · doi:10.1007/978-3-642-77377-8_9 · Cited by 84 · see citing works
Self-citationTwo‐dimensional maps and databases of the human macrophage proteome and secretome
Dupont, A., Tokarski, C., Dekeyzer, O., Guihot, A., Amouyel, P., Rolando, C. H., & Pinet, F. (2004) · PROTEOMICS · doi:10.1002/pmic.200300691 · Cited by 82 · see citing works
Maslin, C., Kedzierska, K., Webster, N., et al. (2005) · Current HIV Research · doi:10.2174/157016205774370401 · Cited by 80 · see citing works
Gupta, S., & Gollapudi, S. V. S. (1993) · Journal of Clinical Immunology · doi:10.1007/bf00920237 · Cited by 75 · see citing works
Mononuclear phagocytes in the pathogenesis of neurodegenerative diseases
Kadiu, I., Glanzer, J. G., Kipnis, J., Gendelman, H. E., & THOMAS, M. P. (2005) · Neurotoxicity Research · doi:10.1007/bf03033818 · Cited by 69 · see citing works
Self-citationCollman, R. G., Perno, C. F., Crowe, S. M., Stevenson, M., & Montaner, L. J. (2003) · Journal of Leukocyte Biology · doi:10.1189/jlb.0703357 · Cited by 67 · see citing works
HIV-1 Nef protects human-monocyte-derived macrophages from HIV-1-induced apoptosis
Olivetta, E., & Federico, M. (2006) · Experimental Cell Research · doi:10.1016/j.yexcr.2005.12.003 · Cited by 55 · see citing works
Transmission of Macrophage‐Tropic HIV‐1 by Breast‐Milk Macrophages via DC‐SIGN
Satomi, M., Shimizu, M., Shinya, E., Watari, E., Owaki, A., Hidaka, C., Ichikawa, M., Takeshita, T., & Takahashi, H. (2005) · The Journal of Infectious Diseases · doi:10.1086/426829 · Cited by 54 · see citing works
Cellular and viral determinants that regulate HIV-1 infection in macrophages
Stevenson, M., & Gendelman, H. E. (1994) · Journal of Leukocyte Biology · doi:10.1002/jlb.56.3.278 · Cited by 45 · see citing works
Self-citationMucosal macrophage subsets of the gut in HIV: decrease in antigen-presenting cell phenotype
Lim, S. G., Condez, A., & Poulter, L. W. (1993) · Clinical & Experimental Immunology · doi:10.1111/j.1365-2249.1993.tb03418.x · Cited by 39 · see citing works
Ciborowski, P. S., Enose, Y., Mack, A., Fladseth, M., & Gendelman, H. E. (2004) · Journal of Neuroimmunology · doi:10.1016/j.jneuroim.2004.08.033 · Cited by 38 · see citing works
Self-citationClaesson, H., & Feinmark, S. J. (1984) · Biochimica et Biophysica Acta (BBA) - Molecular Cell Research · doi:10.1016/0167-4889(84)90098-3 · Cited by 36 · see citing works
CAPSONI, F., Minonzio, F., Ongari, A. M., et al. (1992) · Clinical Immunology and Immunopathology · doi:10.1016/0090-1229(92)90070-5 · Cited by 33 · see citing works
Autoimmunity, anergy, lentiviral immunity and disease
Ansari, A. A. (2004) · Autoimmunity Reviews · doi:10.1016/j.autrev.2004.07.009 · Cited by 24 · see citing works
Rostásy, K. (2005) · Neuropediatrics · doi:10.1055/s-2005-865864 · Cited by 23 · see citing works
Bhoopat, L., Rithaporn, T., Khunamornpong, S., et al. (2005) · Modern Pathology · doi:10.1038/modpathol.3800527 · Cited by 21 · see citing works
Inhibition of HIV-1 replication in primary human monocytes by the IκB-αS32/36A repressor of NF-κB
Palmieri, C., Trimboli, F., Puca, A., et al. (2004) · Retrovirology · doi:10.1186/1742-4690-1-45 · Cited by 17 · see citing works
Microglial oxyradical production: causes and consequences.
Colton, C. A. (1994) · PubMed · Cited by 13 · see citing works
Alveolar macrophage activation in HIV infection
Evans, M. R. W., & Wansbrough-Jones, M. H. (1996) · Journal of Infection · doi:10.1016/s0163-4453(96)92967-9 · Cited by 12 · see citing works
HIV1 Nef: the Machiavelli of cellular activation
Greenway, A. L., & McPhee, D. A. (1997) · Research in Virology · doi:10.1016/s0923-2516(97)81915-2 · Cited by 10 · see citing works
[Tumor necrosis factor (TNF) and oxygen free radicals: potential effects for immunity].
Ferlat, S., & Favier, A. E. (1993) · PubMed · Cited by 5 · see citing works
Ludwik Hirszfeld Memorial Lecture: HIV-1 reservoirs: major molecular obstacles to viral eradication.
Pomerantz, R. J. (2004) · PubMed · Cited by 3 · see citing works
Klegeris (1997) · doi:10.1002/(sici)1097-4547(19970715)49:2
Flagged first, then by citations
Research works printed but not linked: 1
- Gendelman, H.E., Morahan, P.M., 1992. The macrophage in viral infections. In: Lewis, C.E., McGee, J. (Eds.), The Natural Immune System Series: The Macrophage. Oxford Univ. Press, London, pp. 156-232.
Cited by
Sorted by citations
Cellular proteins detected in HIV‐1
Ott, D. E. (2008) · Reviews in Medical Virology · doi:10.1002/rmv.570 · Cited by 141 · see citing works
The Impact of Mass Spectrometry–Based Proteomics on Fundamental Discoveries in Virology
Greco, T. M., Diner, B. A., & Cristea, I. M. (2014) · Annual Review of Virology · doi:10.1146/annurev-virology-031413-085527 · Cited by 67 · see citing works
Global Secretome Characterization of Herpes Simplex Virus 1-Infected Human Primary Macrophages
Miettinen, J. J., Matikainen, S., & Nyman, T. A. (2012) · Journal of Virology · doi:10.1128/jvi.01545-12 · Cited by 48 · see citing works
- Authors
- Ciborowski, P., Kadiu, I., Rozek, W., Smith, L., Bernhardt, K., Fladseth, M., Ricardo-Dukelow, M., & Gendelman, H. E.
- Affiliations
- University of Nebraska Medical Center (US)
- Corresponding author
- Ciborowski, Pawel
- Venue
- Virology
- Publisher
- Elsevier BV
- Published
- 2007-06
- Volume
- 363
- Issue
- 1
- Pages
- 198-209
- ISSN
- 0042-6822 (ISSN-L)
- PMC
- PMC1993545
- OpenAlex
- W1990974472
- Cited by
- 87
- References
- 54
- Citations by year
- 2024: 4 · 2022: 5 · 2021: 3 · 2020: 2 · 2019: 2 · 2018: 1 · 2017: 4 · 2016: 2 · 2015: 6 · 2014: 7 · 2013: 4 · 2012: 9
- FWCI
- 3.91
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Virology (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institutes of Health (5r21mh075662-02, R37 NS36126, 5 R37 NS36126-07)
- National Institute of Mental Health (1 R21 MH075662-01, P01MH064570, 5 R01 MH64570-03, R21 MH075662)
- National Institute of Neurological Disorders and Stroke (5 P01NS31492-11, 5 R37 NS36126-07, R37NS036126, P01NS011766, P01NS031492, 1P01 NS43985-01A1, P01 NS11766-28, P01NS043985, R01NS034239)
- National Center for Research Resources (P20RR015635, 20 RR15635)
- Topics
- Life Sciences › Immunology and Microbiology › Virology › HIV Research and Treatment
- Health Sciences › Medicine › Emergency Medicine › HIV-related health complications and treatments
- Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
- Keywords
- HIV-1, monocyte-derived macrophages, secretome, host-virus interaction, mononuclear phagocytes, cystatin C, cystatin B, YKL-40, cofilin-1, L-plastin, superoxide dismutase, leukotriene A4 hydrolase, alpha-enolase, redox proteins, cytoskeletal proteins, HIV reservoir
- MeSH terms
- Cells, Cultured, Electrophoresis, Polyacrylamide Gel, Gene Expression Regulation, Humans, Macrophages, Monocytes, Proteins, Time Factors, Blotting, Western, HIV-1, Spectrometry, Mass, Electrospray Ionization
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