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Cited by 131Pharmaceutical and Antibiotic Environmental ImpactsAntibiotic Resistance in BacteriaAntibiotic Use and Resistance

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Stormwater loadings of antibiotic resistance genes in an urban stream

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Garner, Emily, Benitez, Romina, von Wagoner, Emily, Sawyer, Richard, Schaberg, Erin, Hession, W. Cully, Krometis, Leigh-Anne H., Badgley, Brian D., Pruden, Amy·2017·Water Research
DOI 10.1016/j.watres.2017.06.046
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Abstract

Antibiotic resistance presents a critical public health challenge and the transmission of antibiotic resistance via environmental pathways continues to gain attention. Factors driving the spread of antibiotic resistance genes (ARGs) in surface water and sources of ARGs in urban stormwater have not been well-characterized. In this study, five ARGs (sul1, sul2, tet(O), tet(W), and erm(F)) were quantified throughout the duration of three storm runoff events in an urban inland stream. Storm loads of all five ARGs were significantly greater than during equivalent background periods. Neither fecal indicator bacteria measured (E. coli or enterococci) was significantly correlated with sul1, sul2, or erm(F), regardless of whether ARG concentration was absolute or normalized to 16S rRNA levels. Both E. coli and enterococci were correlated with the tetracycline resistance genes, tet(O) and tet(W). Next-generation shotgun metagenomic sequencing was conducted to more thoroughly characterize the resistome (i.e., full complement of ARGs) and profile the occurrence of all ARGs described in current databases in storm runoff in order to inform future watershed monitoring and management. Between 37 and 121 different ARGs were detected in each stream sample, though the ARG profiles differed among storms. This study establishes that storm-driven transport of ARGs comprises a considerable fraction of overall downstream loadings and broadly characterizes the urban stormwater resistome to identify p…

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Metadata

Authors
Garner, E., Benitez, R., von Wagoner, E., Sawyer, R., Schaberg, E., Hession, W. C., Krometis, L.-A. H., Badgley, B. D., & Pruden, A.
Affiliations
Virginia Tech (US)
Corresponding author
Garner, Emily
ORCID
Venue
Water Research
Publisher
Elsevier BV
Published
2017-10
Volume
123
Pages
144-152
ISSN
0043-1354 (ISSN-L)
DOI
10.1016/j.watres.2017.06.046
OpenAlex
W2626463301
Cited by
131
References
4
Citations by year
2026: 13 · 2025: 9 · 2024: 17 · 2023: 14 · 2022: 19 · 2021: 22 · 2020: 14 · 2019: 13 · 2018: 8 · 2017: 1
FWCI
4.47
Type
journal article
Language
en
Open access
Licence
Locations
  • Water Research (acceptedVersion)
  • PubMed (publishedVersion)
Funders
  • National Science Foundation (0822220)
  • RAPID (1402651)
  • Virginia Water Resources Research Center Student Grant
  • Virginia Tech Institute for Critical Technology and Applied Science Center for Science and Engineering of the Exposome
  • Virginia Tech College of Agriculture and Life Sciences Integrated Grants Program
  • American Water Works Association Abel Wolman Doctoral Fellowship
  • NSF Partnership for International Research and Education Award (1545756)
Topics
  • Physical Sciences › Environmental Science › Pollution › Pharmaceutical and Antibiotic Environmental Impacts
  • Health Sciences › Medicine › Microbiology (medical) › Antibiotic Resistance in Bacteria
  • Health Sciences › Medicine › Infectious Diseases › Antibiotic Use and Resistance
Keywords
antibiotic resistance genes, urban stormwater, storm runoff, urban rivers, resistome, sul1, sul2, tetO, tetW, ermF, tetracycline resistance, fecal indicator bacteria, Escherichia coli, Enterococcus, watershed management
MeSH terms
Anti-Bacterial Agents, Cities, Drug Resistance, Microbial, Escherichia coli, Genes, Bacterial, RNA, Ribosomal, 16S, Tetracycline, Water Microbiology, Rivers

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