Cited by 51Marine Invertebrate Physiology and EcologyDevelopmental Biology and Gene RegulationPlanarian Biology and Electrostimulation

Notch-signalling is required for head regeneration and tentacle patterning in Hydra

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Münder, Sandra, Tischer, Susanne, Grundhuber, Maresa, Büchels, Nathalie, Bruckmeier, Nadine, Eckert, Stefanie, Seefeldt, Carolin A., Prexl, Andrea +2 more·2013·Developmental Biology

Abstract

Local self-activation and long ranging inhibition provide a mechanism for setting up organising regions as signalling centres for the development of structures in the surrounding tissue. The adult hydra hypostome functions as head organiser. After hydra head removal it is newly formed and complete heads can be regenerated. The molecular components of this organising region involve Wnt-signalling and β-catenin. However, it is not known how correct patterning of hypostome and tentacles are achieved in the hydra head and whether other signals in addition to HyWnt3 are needed for re-establishing the new organiser after head removal. Here we show that Notch-signalling is required for re-establishing the organiser during regeneration and that this is due to its role in restricting tentacle activation. Blocking Notch-signalling leads to the formation of irregular head structures characterised by excess tentacle tissue and aberrant expression of genes that mark the tentacle boundaries. This indicates a role for Notch-signalling in defining the tentacle pattern in the hydra head. Moreover, lateral inhibition by HvNotch and its target HyHes are required for head regeneration and without this the formation of the β-catenin/Wnt dependent head organiser is impaired. Work on prebilaterian model organisms has shown that the Wnt-pathway is important for setting up signalling centres for axial patterning in early multicellular animals. Our data suggest that the integration of Wnt-signalling with Notch-Delta activity was also involved in the evolution of defined body plans in animals.

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Crossref · 10.1016/j.ydbio.2013.08.022

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  • Notch signalling in bud detachment and tentacle formation during regeneration are fundamentally different processes from neuronal differentiation.

    “detachment of the bud and in tentacle formation during regeneration [66], thus, fundamentally different processes from neuronal differentiation.”
  • In Hydra, chemical inhibition of Notch signalling suggests a role in boundary formation during detachment of the bud and in tentacle formation during regeneration, processes fundamentally different from neuronal differentiation.

    “Notably, in hydra, chemical inhibition of Notch signalling suggests a role in boundary formation during detachment of the bud and in tentacle formation during regeneration [66], thus, fundamentally different processes from neuronal differentiation.”

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Authors
Münder, S., Tischer, S., Grundhuber, M., Büchels, N., Bruckmeier, N., Eckert, S., Seefeldt, C. A., Prexl, A., Käsbauer, T., & Böttger, A.
Affiliations
Ludwig-Maximilians-Universität München (DE)
Corresponding author
Böttger, Angelika
Venue
Developmental Biology
Publisher
Elsevier BV
Published
2013-11
Volume
383
Issue
1
Pages
146-157
ISSN
0012-1606 (ISSN-L)
OpenAlex
W1975337787
Cited by
51
References
46
Citations by year
2026: 1 · 2025: 1 · 2024: 8 · 2023: 1 · 2022: 4 · 2021: 9 · 2020: 5 · 2019: 9 · 2018: 1 · 2017: 3 · 2016: 1 · 2015: 3 · 2014: 4 · 2013: 1
FWCI
2.03
Type
journal article
Language
en
Open access
Locations
  • Developmental Biology (publishedVersion)
  • PubMed (publishedVersion)
Funders
DFG (BO1748-5)
Topics
  • Physical Sciences › Earth and Planetary Sciences › Paleontology › Marine Invertebrate Physiology and Ecology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Planarian Biology and Electrostimulation
Keywords
Notch signaling, Hydra, head regeneration, hypostome, lateral inhibition, Wnt signaling, β-catenin, body plan evolution, axial patterning
MeSH terms
Animals, Bromodeoxyuridine, Dipeptides, Extremities, Head, Hydra, Regeneration, Signal Transduction, In Situ Hybridization, DNA Primers, Microscopy, Confocal, Receptors, Notch

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