Membrane progestin receptors in the midbrain ventral tegmental area are required for progesterone-facilitated lordosis of rats
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Progesterone (P₄) and its metabolites, rapidly facilitate lordosis of rats partly through actions in the ventral tegmental area (VTA). The study of membrane progestin receptors (mPRs), of the Progestin and AdipoQ Receptor (PAQR) superfamily, has been limited to expression and regulation, instead of function. We hypothesized that if mPRs are required for progestin-facilitated lordosis in the VTA, then mPRs will be expressed in this region and knockdown will attenuate lordosis. First, expression of mPR was examined by reverse-transcriptase polymerase chain reaction (RT-PCR) in brain and peripheral tissues of proestrous Long-Evans rats. Expression of mPRα (paqr7) was observed in peripheral tissues and brain areas, including hypothalamus and midbrain. Expression of mPRβ (paqr8) was observed in brain tissues and was abundant in the midbrain and hypothalamus. Second, ovariectomized rats were estrogen (E₂; 0.09 mg/kg, SC), and P₄ (4 mg/kg, SC) or vehicle-primed, and infused with antisense oligodeoxynucleotides (AS-ODNs) targeted against mPRα and/or mPRβ intracerebroventricularly or to the VTA. Rats were assessed for motor (open field), anxiety (elevated plus maze), social (social interaction), and sexual (lordosis) behavior. P₄-facilitated lordosis was significantly reduced with administration of AS-ODNs for mPRα, mPRβ, or co-administration of mPRα and mPRβ to the lateral ventricle, compared to vehicle. P₄-facilitated lordosis was reduced, compared to vehicle, by administration of mPRβ AS-ODNs, or co-administration of mPRα and mPRβ AS-ODNs, but not mPRα AS-ODNs alone, to the VTA. No differences were observed for motor, anxiety, or social behaviors. Thus, mPRs in the VTA are targets of progestin-facilitated lordosis of rats.
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Many cells in the ventral tegmental area also express tyrosine hydroxylase, suggesting they produce dopamine.
“Many of these cells also express tyrosine hydroxylase, suggesting they produce dopamine.”
From Does puberty mark a transition in sensitive periods for plasticity in the associative neocortex? (2017) · cites this paper as Frye et al., 2013
In adult rodents, the ventral tegmental area expresses steroid receptors in specific spatial and concentration gradients, including progesterone receptor, androgen receptor, and both estrogen receptor alpha and beta.
“but in adult rodents this area expresses steroid receptors in specific spatial and concentration gradients, including progesterone receptor (Frye et al., 2013; Willing and Wagner, 2016), androgen receptor (Kritzer, 1997; Kritzer and Creutz, 2008) and both estrogen receptor alpha and beta ((Creutz and Kritzer, 2002; Kritzer, 1997; Vanderhorst et al., 2005).”
From Does puberty mark a transition in sensitive periods for plasticity in the associative neocortex? (2017) · cites this paper as Frye et al., 2013
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Does puberty mark a transition in sensitive periods for plasticity in the associative neocortex?
Piekarski, D. J., Johnson, C. M., Boivin, J. R., Thomas, A. W., Lin, W. C., Delevich, K., M. Galarce, E., & Wilbrecht, L. (2017) · Brain Research · doi:10.1016/j.brainres.2016.08.042 · Cited by 175 · see citing works
Non-genomic mechanisms of progesterone action in the brain
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- Authors
- Frye, C. A., Walf, A. A., Kohtz, A. S., & Zhu, Y.
- Affiliations
- University of Alaska Fairbanks (US)
- University at Albany, State University of New York (US)
- East Carolina University (US)
- Corresponding author
- Frye, Cheryl A.
- Venue
- Hormones and Behavior
- Publisher
- Elsevier BV
- Published
- 2013-08
- Volume
- 64
- Issue
- 3
- Pages
- 539-545
- ISSN
- 0018-506X (ISSN-L)
- PMC
- PMC4541797
- OpenAlex
- W2005353812
- Cited by
- 27
- References
- 68
- Citations by year
- 2026: 2 · 2025: 1 · 2024: 3 · 2023: 1 · 2022: 4 · 2021: 2 · 2020: 2 · 2019: 1 · 2018: 1 · 2017: 1 · 2016: 4 · 2015: 1 · 2014: 1 · 2013: 3
- FWCI
- 1.99
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Hormones and Behavior (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- NSF (IOS-0957148)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Estrogen and related hormone effects
- Health Sciences › Medicine › Reproductive Medicine › Hypothalamic control of reproductive hormones
- Social Sciences › Psychology › Social Psychology › Neuroendocrine regulation and behavior
- Keywords
- progesterone, lordosis, ventral tegmental area, antisense oligodeoxynucleotides, sexual behavior, midbrain, hypothalamus, anxiety-like behavior, social behavior
- MeSH terms
- Animals, Cell Membrane, Female, Male, Mesencephalon, Posture, Progesterone, Receptors, Progesterone, Sexual Behavior, Animal, Gene Expression, Ventral Tegmental Area, Rats, Long-Evans, Oligodeoxyribonucleotides, Antisense, Rats, Infusions, Intraventricular
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