Bakers' Yeast, a Model for Fungal Biofilm Formation
Abstract
Biofilms are formed by the aggregation of microorganisms into multicellular structures that adhere to surfaces. Here we show that bakers' yeast Saccharomyces cerevisiae can initiate biofilm formation. When grown in low-glucose medium, the yeast cells adhered avidly to a number of plastic surfaces. On semi-solid (0.3% agar) medium they formed “mats”: complex multicellular structures composed of yeast-form cells. Both attachment to plastic and mat formation require Flo11p, a member of a large family of fungal cell surface glycoproteins involved in adherence. The ability to study biofilm formation in a tractable genetic system may facilitate the identification of new targets for antifungal therapy.
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Saccharomyces cerevisiae is able to form a thin biofilm consisting of round, budding yeast-form cells and pseudohyphal cells in vitro.
“and it is able to form a thin biofilm consisting of round, budding yeast-form cells and pseudohyphal cells in vitro (26, 50, 128, 164).”
From <i>Candida albicans</i> Biofilms and Human Disease (2015) · cites this paper as [164]
Saccharomyces cerevisiae has been implicated in catheter-associated infections with mixed-species biofilms in patients in intensive care units.
“In a few case studies, however, S. cerevisiae has been implicated in catheter-associated infections with mixed-species biofilms in patients in intensive care units (ICUs)”
From <i>Candida albicans</i> Biofilms and Human Disease (2015) · cites this paper as [164]
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- Authors
- Reynolds, T. B., & Fink, G. R.
- Affiliations
- Whitehead Institute for Biomedical Research (US)
- Corresponding author
- Fink, Gerald R.
- Venue
- Science
- Published
- 2001-02-02
- Volume
- 291
- Issue
- 5505
- Pages
- 878-881
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2032674459
- Cited by
- 585
- References
- 16
- Citations by year
- 2026: 7 · 2025: 9 · 2024: 17 · 2023: 23 · 2022: 24 · 2021: 20 · 2020: 21 · 2019: 28 · 2018: 28 · 2017: 21 · 2016: 26 · 2015: 28 · 2014: 32 · 2013: 32 · 2012: 30
- FWCI
- 7.10
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Science (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute of General Medical Sciences (GM20565, R01GM040266, F32GM020565, 5 RO1 GM40266)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
- Keywords
- biofilm formation, Saccharomyces cerevisiae, cell surface glycoproteins, cell adhesion, multicellular structures, antifungal drug targets
- MeSH terms
- Mating Factor, Agar, Cell Adhesion, Culture Media, Fungal Proteins, Genes, Fungal, Glucose, Lipoproteins, Membrane Glycoproteins, Membrane Proteins, Nuclear Proteins, Peptides, Pheromones, Plastics, Ploidies, Saccharomyces cerevisiae, Trans-Activators, Biofilms, MAP Kinase Signaling System, Saccharomyces cerevisiae Proteins
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