The DOI matches its Crossref record; 59 of the 59 references it cites were found in a registry or an index.
Evolutionary engineered Saccharomyces cerevisiae wine yeast strains with increased in vivo flux through the pentose phosphate pathway
Abstract
Amplification of the flux toward the pentose phosphate (PP) pathway might be of interest for various S. cerevisiae based industrial applications. We report an evolutionary engineering strategy based on a long-term batch culture on gluconate, a substrate that is poorly assimilated by S. cerevisiae cells and is metabolized by the PP pathway. After adaptation for various periods of time, we selected strains that had evolved a greater consumption capacity for gluconate. (13)C metabolic flux analysis on glucose revealed a redirection of carbon flux from glycolysis towards the PP pathway and a greater synthesis of lipids. The relative flux into the PP pathway was 17% for the evolved strain (ECA5) versus 11% for the parental strain (EC1118). During wine fermentation, the evolved strains displayed major metabolic changes, such as lower levels of acetate production, higher fermentation rates and enhanced production of aroma compounds. These represent a combination of novel traits, which are of great interest in the context of modern winemaking.
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“Furthermore, experiments on pentose utilization highlighted that increased gene copy numbers of the heterologous genes in recombinant S. cerevisiae can emerge during evolutionary engineering and contribute to increased growth in anaerobic conditions [104,109].”
From Adaptive laboratory evolution—Principles and applications for biotechnology (2013) · cites this paper as [109]
In the citing paper
“For example, several studies improved the carbon source utilization of S. cerevisiae strains by genetic engineering in combination with evolutionary adaptation [104,107-109].”
From Adaptive laboratory evolution—Principles and applications for biotechnology (2013) · cites this paper as [109]
Citations
Cites
A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION
Bligh, E. G., & Dyer, W. J. (1959) · Canadian Journal of Biochemistry and Physiology · doi:10.1139/o59-099 · Cited by 54,751
The ade4 Package: Implementing the Duality Diagram for Ecologists
Dray, S., & Dufour, A. (2007) · Journal of Statistical Software · doi:10.18637/jss.v022.i04 · Cited by 6,740
Yeasts : characteristics and identification
Barnett, J. A., Payne, R. W., Yarrow, D., & Barnett, L. (2000) · Cited by 1,870
A method for the analysis of sugars in plant cell-wall polysaccharides by gas-liquid chromatography
Albersheim, P., Nevins, D. J., English, P. D., & Karr, A. L. (1967) · Carbohydrate Research · doi:10.1016/s0008-6215(00)80510-8 · Cited by 1,648
Yeasts: Characteristics and identification
Spencer, J. F. T. (1984) · Food Microbiology · doi:10.1016/0740-0020(84)90068-6 · Cited by 680
Roberts, R. L., & Fink, G. R. (1994) · Genes & Development · doi:10.1101/gad.8.24.2974 · Cited by 616
Ribéreau‐Gayon, P., Dubourdieu, D., Donèche, B. B., & Lonvaud‐Funel, A. (2005) · Wiley eBooks · doi:10.1002/0470010363 · Cited by 601
An improved procedure for the methylation analysis of oligosaccharides and polysaccharides
Harris, P. J., Henry, R. J., Blakeney, A. B., & Stone, B. (1984) · Carbohydrate Research · doi:10.1016/0008-6215(84)85106-x · Cited by 587
Bakers' Yeast, a Model for Fungal Biofilm Formation
Reynolds, T. B., & Fink, G. R. (2001) · Science · doi:10.1126/science.291.5505.878 · Cited by 585
Ribéreau‐Gayon, P., Dubourdieu, D., Donèche, B. B., Lonvaud‐Funel, A., Darriet, P., & Towey, J. P. (2021) · Wiley eBooks · doi:10.1002/9781119588320 · Cited by 556
Cited by
Sorted by citations
The return of metabolism: biochemistry and physiology of the pentose phosphate pathway
Stincone, A., Prigione, A., Cramer, T., Wamelink, M. M. C., Campbell, K., Cheung, E., Olin‐Sandoval, V., Grüning, N., Krüger, A., Tauqeer Alam, M., et al. (2015) · Biological Reviews · doi:10.1111/brv.12140 · Cited by 1,576
Adaptive laboratory evolution—Principles and applications for biotechnology
Dragosits, M., & Mattanovich, D. (2013) · Microbial Cell Factories · doi:10.1186/1475-2859-12-64 · Cited by 827
Sandberg, T. E., Salazar, M. J., Weng, L. L., Palsson, B. Ø., & Feist, A. M. (2019) · Metabolic Engineering · doi:10.1016/j.ymben.2019.08.004 · Cited by 585
Improving industrial yeast strains: exploiting natural and artificial diversity
Steensels, J., Snoek, T., Meersman, E., Nicolino, M. P., Voordeckers, K., & Verstrepen, K. J. (2014) · FEMS Microbiology Reviews · doi:10.1111/1574-6976.12073 · Cited by 513
Adaptive laboratory evolution principles and applications in industrial biotechnology
Mavrommati, M., Daskalaki, A., Papanikolaou, S., & Aggelis, G. (2021) · Biotechnology Advances · doi:10.1016/j.biotechadv.2021.107795 · Cited by 272
Metadata
- Authors
- Cadière, A., Ortiz-Julien, A., Camarasa, C., & Dequin, S.
- Affiliations
- Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement (FR)
- Lallemand (France) (FR)
- Corresponding author
- Dequin, Sylvie
- Venue
- Metabolic Engineering
- Publisher
- Elsevier BV
- Published
- 2011-05
- Volume
- 13
- Issue
- 3
- Pages
- 263-271
- ISSN
- 1096-7176 (ISSN-L)
- OpenAlex
- W2058737346
- Cited by
- 141
- References
- 59
- Citations by year
- 2026: 4 · 2025: 7 · 2024: 12 · 2023: 7 · 2022: 3 · 2021: 8 · 2020: 9 · 2019: 8 · 2018: 9 · 2017: 17 · 2016: 10 · 2015: 9 · 2014: 18 · 2013: 7 · 2012: 8
- FWCI
- 4.92
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Metabolic Engineering (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion)
- Funders
- Agence Nationale de la Recherche (ANR-05-PNRA-0011)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Life Sciences › Agricultural and Biological Sciences › Food Science › Fermentation and Sensory Analysis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Keywords
- Saccharomyces cerevisiae, wine yeast, pentose phosphate pathway, evolutionary engineering, metabolic flux redirection, lipogenesis, acetate production, aroma production, fermentation rate, 13C metabolic flux analysis, industrial yeast strains
- MeSH terms
- Gluconates, Glucose, Glycolysis, Pentose Phosphate Pathway, Saccharomyces cerevisiae, Wine, Directed Molecular Evolution
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
