The DOI matches its Crossref record; 54 of the 56 references it cites were found in a registry or an index.
Engineering cyanobacteria for photosynthetic production of 3-hydroxybutyrate directly from CO2
Abstract
(S)- and (R)-3-hydroxybutyrate (3HB) are precursors to synthesize the biodegradable plastics polyhydroxyalkanoates (PHAs) and many fine chemicals. To date, however, their production has been restricted to petroleum-based chemical industry and sugar-based microbial fermentation, limiting its sustainability and economical feasibility. With the ability to fix CO2 photosynthetically, cyanobacteria have attracted increasing interest as a biosynthesis platform to produce fuels and chemicals from alternative renewable resources. To this end, synthesis metabolic pathways have been constructed and optimized in cyanobacterium Synechocystis sp. PCC 6803 to photosynthetically produce (S)- and (R)-3HB directly from CO2. Both types of 3HB molecules were produced and readily secreted from Synechocystis cells without over-expression of transporters. Additional inactivation of the competing pathway by deleting slr1829 and slr1830 (encoding PHB polymerase) from the Synechocystis genome further promoted the 3HB production. Up to 533.4mg/L 3HB has been produced after photosynthetic cultivation of the engineered cyanobacterium Synechocystis TABd for 21 days. Further analysis indicated that the phosphate consumption during the photoautrophic growth and the concomitant elevated acetyl-CoA pool acted as a key driving force for 3HB biosynthesis in Synechocystis. For the first time, the study has demonstrated the feasibility of photosynthetic production of (S)- and (R)-3HB directly from sunlight and CO2.
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“This strain has become a key experimental platform due to its relatively fast growth, well-characterised physiology, and the availability of established genetic manipulation techniques [59,60].”
From Cyanobacteria as a Photosynthetic Chassis for Metabolic Pathway Engineering with Heterologous Gene Expression (2026) · cites this paper as [60]
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Cites
Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method
Livak, K. J., & Schmittgen, T. D. (2001) · Methods · doi:10.1006/meth.2001.1262 · Cited by 180,860
Chisti, Y. (2007) · Biotechnology Advances · doi:10.1016/j.biotechadv.2007.02.001 · Cited by 9,524
Generic Assignments, Strain Histories and Properties of Pure Cultures of Cyanobacteria
Rippka, R., Stanier, R. Y., Deruelles, J., Herdman, M., & Waterbury, J. B. (1979) · Microbiology · doi:10.1099/00221287-111-1-1 · Cited by 7,606
Kaneko, T., Sato, S., Kotani, H., Tanaka, A., Asamizu, E., Nakamura, Y., Miyajima, N., Hirosawa, M., Sugiura, M., Sasamoto, S., et al. (1996) · DNA Research · doi:10.1093/dnares/3.3.185 · Cited by 2,434
Kaneko, T., Sato, S., Kotani, H., Tanaka, A., Asamizu, E., Nakamura, Y., Miyajima, N., Hirosawa, M., Sugiura, M., Sasamoto, S., et al. (1996) · DNA Research · doi:10.1093/dnares/3.3.109 · Cited by 2,132
Metabolic Engineering of Poly(3-Hydroxyalkanoates): From DNA to Plastic
Madison, L. L., & Huisman, G. W. (1999) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.63.1.21-53.1999 · Cited by 1,598
Codon usage tabulated from international DNA sequence databases: status for the year 2000
Nakamura, Y. (2000) · Nucleic Acids Research · doi:10.1093/nar/28.1.292 · Cited by 1,259
Direct photosynthetic recycling of carbon dioxide to isobutyraldehyde
Atsumi, S., Higashide, W., & Liao, J. C. (2009) · Nature Biotechnology · doi:10.1038/nbt.1586 · Cited by 870
Genome sequence of the bioplastic-producing “Knallgas” bacterium Ralstonia eutropha H16
Pohlmann, A., Fricke, W. F., Reinecke, F., Kusian, B., Liesegang, H., Cramm, R., Eitinger, T., Ewering, C., Pötter, M., Schwartz, E., et al. (2006) · Nature Biotechnology · doi:10.1038/nbt1244 · Cited by 662
Lindberg, P., Park, S., & Melis, A. (2010) · Metabolic Engineering · doi:10.1016/j.ymben.2009.10.001 · Cited by 596
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Aresta, M., Dibenedetto, A., & Quaranta, E. (2016) · Journal of Catalysis · doi:10.1016/j.jcat.2016.04.003 · Cited by 354
Atmospheric CO2 mitigation technologies: carbon capture utilization and storage
Nocito, F., & Dibenedetto, A. (2019) · Current Opinion in Green and Sustainable Chemistry · doi:10.1016/j.cogsc.2019.10.002 · Cited by 351
Calero, P., & Nikel, P. I. (2018) · Microbial Biotechnology · doi:10.1111/1751-7915.13292 · Cited by 331
Synthetic biology of cyanobacteria: unique challenges and opportunities
Berla, B. M., Saha, R., Immethun, C. M., Maranas, C. D., Moon, T. S., & Pakrasi, H. B. (2013) · Frontiers in Microbiology · doi:10.3389/fmicb.2013.00246 · Cited by 305
Metabolic engineering of cyanobacteria for the synthesis of commodity products
Angermayr, S. A., Gorchs Rovira, A., & Hellingwerf, K. J. (2015) · Trends in Biotechnology · doi:10.1016/j.tibtech.2015.03.009 · Cited by 273
Metadata
- Authors
- Wang, B., Pugh, S., Nielsen, D. R., Zhang, W., & Meldrum, D. R.
- Affiliations
- Arizona State University (US)
- Corresponding author
- Zhang, Weiwen
- Meldrum, Deirdre R.
- Venue
- Metabolic Engineering
- Publisher
- Elsevier BV
- Published
- 2013-03
- Volume
- 16
- Pages
- 68-77
- ISSN
- 1096-7176 (ISSN-L)
- OpenAlex
- W2012010696
- Cited by
- 170
- References
- 56
- Citations by year
- 2026: 3 · 2025: 8 · 2024: 9 · 2023: 6 · 2022: 15 · 2021: 17 · 2020: 7 · 2019: 12 · 2018: 14 · 2017: 17 · 2016: 17 · 2015: 24 · 2014: 13 · 2013: 8
- FWCI
- 9.25
- Type
- journal article
- Language
- en
- Update notices
- erratum (2014-01-01)
- Open access
- closed
- Locations
- Metabolic Engineering (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Petroleum Technology Alliance Canada
- National Key Research and Development Program of China
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Physical Sciences › Materials Science › Biomaterials › biodegradable polymer synthesis and properties
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Keywords
- beta-hydroxybutyrate, cyanobacteria, Synechocystis sp. PCC 6803, photosynthetic carbon fixation, polyhydroxyalkanoates, biodegradable plastics, metabolic pathway engineering, phosphate consumption, acetyl-CoA pool, photoautotrophic cultivation
- MeSH terms
- Bacterial Proteins, Carrier Proteins, Photosynthesis, Gene Expression, Gene Deletion, 3-Hydroxybutyric Acid, Synechocystis, Metabolic Engineering
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