Engineering a platform for photosynthetic isoprene production in cyanobacteria, using Synechocystis as the model organism

Lindberg, Pia; Park, Sungsoon; Melis, Anastasios

doi:10.1016/j.ymben.2009.10.001

Cited by 598Microbial Metabolic Engineering and BioproductionPlant biochemistry and biosynthesisAlgal biology and biofuel production

Engineering a platform for photosynthetic isoprene production in cyanobacteria, using Synechocystis as the model organism

Lindberg, Pia, Park, Sungsoon, Melis, Anastasios·2010·Metabolic Engineering

DOI 10.1016/j.ymben.2009.10.001

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Abstract

The concept of "photosynthetic biofuels" envisions application of a single organism, acting both as photo-catalyst and producer of ready-made fuel. This concept was applied upon genetic engineering of the cyanobacterium Synechocystis, conferring the ability to generate volatile isoprene hydrocarbons from CO(2) and H(2)O. Heterologous expression of the Pueraria montana (kudzu) isoprene synthase (IspS) gene in Synechocystis enabled photosynthetic isoprene generation in these cyanobacteria. Codon-use optimization of the kudzu IspS gene improved expression of the isoprene synthase in Synechocystis. Use of the photosynthesis psbA2 promoter, to drive the expression of the IspS gene, resulted in a light-intensity-dependent isoprene synthase expression. Results showed that oxygenic photosynthesis can be re-directed to generate useful small volatile hydrocarbons, while consuming CO(2), without a prior requirement for the harvesting, dewatering and processing of the respective biomass.

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Synechocystis PCC 6803 was genetically modified with the Pueraria montana ispS gene to enable production of isoprene.
“In a recent study, Synechocystis PCC 6803 was genetically modified with the Pueraria montana ispS gene to enable the production of isoprene in this microorganism (Lindberg et al. 2010).”
From Renewable energy from Cyanobacteria: energy production optimization by metabolic pathway engineering (2011) · cites this paper as Lindberg et al. 2010
Previous work has shown that a minimum of the carbon derived from photosynthesis is directed to pathways involved in biofuel production.
“Previous work has shown that a minimum of the C derived from photosynthesis is directed to pathways involved in the biofuel production (Lindberg et al. 2010).”
From Renewable energy from Cyanobacteria: energy production optimization by metabolic pathway engineering (2011) · cites this paper as Lindberg et al. 2010

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Authors: Lindberg, P., Park, S., & Melis, A.
Affiliations: University of California, Berkeley (US)
Corresponding author: Melis, Anastasios
ORCID: Lindberg, Pia
Melis, Anastasios
Venue: Metabolic Engineering
Publisher: Elsevier BV
Published: 2010-01
Volume: 12
Issue: 1
Pages: 70-79
ISSN: 1096-7176 (ISSN-L)
DOI: 10.1016/j.ymben.2009.10.001
OpenAlex: W2032641946
Cited by: 596
References: 44
Citations by year: 2026: 19 · 2025: 17 · 2024: 19 · 2023: 29 · 2022: 34 · 2021: 42 · 2020: 39 · 2019: 21 · 2018: 44 · 2017: 40 · 2016: 57 · 2015: 53 · 2014: 57 · 2013: 51 · 2012: 38
FWCI: 9.89
Type: journal article
Language: en
Open access: closed
Licence: www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations: Metabolic Engineering (publishedVersion)
PubMed (publishedVersion)
eScholarship (California Digital Library) (submittedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant biochemistry and biosynthesis
Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
Keywords: cyanobacteria, Synechocystis, isoprene synthase, Pueraria lobata, codon optimization, heterologous expression, volatile hydrocarbons, oxygenic photosynthesis
MeSH terms: Bacterial Proteins, Butadienes, Pentanes, Photosynthesis, Plant Proteins, Promoter Regions, Genetic, Alkyl and Aryl Transferases, Pueraria, Photosystem II Protein Complex, Hemiterpenes, Synechocystis

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Engineering a platform for photosynthetic isoprene production in cyanobacteria, using Synechocystis as the model organism

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