Cited by 170Microbial Metabolic Engineering and Bioproductionbiodegradable polymer synthesis and propertiesAlgal biology and biofuel production

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Engineering cyanobacteria for photosynthetic production of 3-hydroxybutyrate directly from CO2

Wang, Bo, Pugh, Shawn, Nielsen, David R., Zhang, Weiwen, Meldrum, Deirdre R.·2013·Metabolic Engineering

DOI 10.1016/j.ymben.2013.01.001

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Abstract

(S)- and (R)-3-hydroxybutyrate (3HB) are precursors to synthesize the biodegradable plastics polyhydroxyalkanoates (PHAs) and many fine chemicals. To date, however, their production has been restricted to petroleum-based chemical industry and sugar-based microbial fermentation, limiting its sustainability and economical feasibility. With the ability to fix CO2 photosynthetically, cyanobacteria have attracted increasing interest as a biosynthesis platform to produce fuels and chemicals from alternative renewable resources. To this end, synthesis metabolic pathways have been constructed and optimized in cyanobacterium Synechocystis sp. PCC 6803 to photosynthetically produce (S)- and (R)-3HB directly from CO2. Both types of 3HB molecules were produced and readily secreted from Synechocystis cells without over-expression of transporters. Additional inactivation of the competing pathway by deleting slr1829 and slr1830 (encoding PHB polymerase) from the Synechocystis genome further promoted the 3HB production. Up to 533.4mg/L 3HB has been produced after photosynthetic cultivation of the engineered cyanobacterium Synechocystis TABd for 21 days. Further analysis indicated that the phosphate consumption during the photoautrophic growth and the concomitant elevated acetyl-CoA pool acted as a key driving force for 3HB biosynthesis in Synechocystis. For the first time, the study has demonstrated the feasibility of photosynthetic production of (S)- and (R)-3HB directly from sunlight and CO2.

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Authors: Wang, B., Pugh, S., Nielsen, D. R., Zhang, W., & Meldrum, D. R.
Affiliations: Arizona State University (US)
Corresponding author: Zhang, Weiwen
Meldrum, Deirdre R.
ORCID: Wang, Bo
Nielsen, David R.
Zhang, Weiwen
Venue: Metabolic Engineering
Publisher: Elsevier BV
Published: 2013-03
Volume: 16
Pages: 68-77
ISSN: 1096-7176 (ISSN-L)
DOI: 10.1016/j.ymben.2013.01.001
OpenAlex: W2012010696
Cited by: 170
References: 56
Citations by year: 2026: 3 · 2025: 8 · 2024: 9 · 2023: 6 · 2022: 15 · 2021: 17 · 2020: 7 · 2019: 12 · 2018: 14 · 2017: 17 · 2016: 17 · 2015: 24 · 2014: 13 · 2013: 8
FWCI: 9.25
Type: journal article
Language: en
Update notices: erratum (2014-01-01)
Open access: closed
Licence: www.elsevier.com/tdm/userlicense/1.0/ (tdm)
www.elsevier.com/legal/tdmrep-license (tdm)
Locations: Metabolic Engineering (publishedVersion)
PubMed (publishedVersion)
Funders: Petroleum Technology Alliance Canada
National Key Research and Development Program of China
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
Physical Sciences › Materials Science › Biomaterials › biodegradable polymer synthesis and properties
Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
Keywords: beta-hydroxybutyrate, cyanobacteria, Synechocystis sp. PCC 6803, photosynthetic carbon fixation, polyhydroxyalkanoates, biodegradable plastics, metabolic pathway engineering, phosphate consumption, acetyl-CoA pool, photoautotrophic cultivation
MeSH terms: Bacterial Proteins, Carrier Proteins, Photosynthesis, Gene Expression, Gene Deletion, 3-Hydroxybutyric Acid, Synechocystis, Metabolic Engineering

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Engineering cyanobacteria for photosynthetic production of 3-hydroxybutyrate directly from CO2

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