Metabolic engineering of cyanobacteria for the synthesis of commodity products

Angermayr, S. Andreas; Gorchs Rovira, Aleix; Hellingwerf, Klaas J.

doi:10.1016/j.tibtech.2015.03.009

Cited by 273Microbial Metabolic Engineering and BioproductionAlgal biology and biofuel productionPhotosynthetic Processes and Mechanisms

Metabolic engineering of cyanobacteria for the synthesis of commodity products

Angermayr, S. Andreas, Gorchs Rovira, Aleix, Hellingwerf, Klaas J.·2015·Trends in Biotechnology

DOI 10.1016/j.tibtech.2015.03.009

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Abstract

Through metabolic engineering cyanobacteria can be employed in biotechnology. Combining the capacity for oxygenic photosynthesis and carbon fixation with an engineered metabolic pathway allows carbon-based product formation from CO(2), light, and water directly. Such cyanobacterial 'cell factories' are constructed to produce biofuels, bioplastics, and commodity chemicals. Efforts of metabolic engineers and synthetic biologists allow the modification of the intermediary metabolism at various branching points, expanding the product range. The new biosynthesis routes 'tap' the metabolism ever more efficiently, particularly through the engineering of driving forces and utilization of cofactors generated during the light reactions of photosynthesis, resulting in higher product titers. High rates of carbon rechanneling ultimately allow an almost-complete allocation of fixed carbon to product above biomass.

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Native regulatory networks resist carbon flux redirection.
“native regulatory networks resist carbon flux redirection”
From Cyanobacteria as a Photosynthetic Chassis for Metabolic Pathway Engineering with Heterologous Gene Expression (2026) · cites this paper as [19]
Cultivation constraints-including light attenuation in dense cultures and mismatches between photosynthetic ATP/NADPH supply and heterologous pathway demand-limit achievable yields.
“cultivation constraints—including light attenuation in dense cultures and mismatches between photosynthetic ATP/NADPH supply and heterologous pathway demand—further limit achievable yields.”
From Cyanobacteria as a Photosynthetic Chassis for Metabolic Pathway Engineering with Heterologous Gene Expression (2026) · cites this paper as [19]

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Engineering synergetic CO2-fixing pathways for malate production
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A Hybrid Flux Balance Analysis and Machine Learning Pipeline Elucidates Metabolic Adaptation in Cyanobacteria
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Fusion of Ferredoxin and Cytochrome P450 Enables Direct Light-Driven Biosynthesis
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Strategies to Obtain Designer Polymers Based on Cyanobacterial Extracellular Polymeric Substances (EPS)
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System metabolic engineering of exopolysaccharide-producing cyanobacteria in soil rehabilitation by inducing the formation of biological soil crusts: A review
Tiwari, O. N., Bhunia, B., Mondal, A., Gopikrishna, K., & Indrama, T. (2018) · Journal of Cleaner Production · doi:10.1016/j.jclepro.2018.11.188 · Cited by 63 · see citing works
Biocatalysis in Green and Blue: Cyanobacteria
Jodlbauer, J., Rohr, T., Oliver Spadiut, A., Mihovilovič, M. D., & Rudroff, F. (2021) · Trends in biotechnology · doi:10.1016/j.tibtech.2020.12.009 · Cited by 63 · see citing works
The Many Roles of the Bacterial Second Messenger Cyclic di-AMP in Adapting to Stress Cues
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Hints at the Applicability of Microalgae and Cyanobacteria for the Biodegradation of Plastics
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Order-of-magnitude enhancement in photocurrent generation of Synechocystis sp. PCC 6803 by outer membrane deprivation
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Tunable Repression of Key Photosynthetic Processes Using Cas12a CRISPR Interference in the Fast-Growing Cyanobacterium Synechococcus sp. UTEX 2973
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Identifying the Metabolic Differences of a Fast-Growth Phenotype in Synechococcus UTEX 2973
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Synthetic Biology Toolkits for Metabolic Engineering of Cyanobacteria
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MoS2 Nanosheets–Cyanobacteria Interaction: Reprogrammed Carbon and Nitrogen Metabolism
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Systematic identification and elimination of flux bottlenecks in the aldehyde production pathway of Synechococcus elongatus PCC 7942
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Redirecting photosynthetic electron flux in the cyanobacterium Synechocystis sp. PCC 6803 by the deletion of flavodiiron protein Flv3
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The primary transcriptome of the fast-growing cyanobacterium Synechococcus elongatus UTEX 2973
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Photomixotrophic chemical production in cyanobacteria
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A balanced ATP driving force module for enhancing photosynthetic biosynthesis of 3-hydroxybutyrate from CO2
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Biocontainment of Genetically Engineered Algae
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100 Years Later, What Is New in Glycerol Bioproduction?
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Unlocking the photobiological conversion of CO 2 to ( R )-3-hydroxybutyrate in cyanobacteria
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A tightly inducible riboswitch system in Synechocystis sp. PCC 6803
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Microbial autotrophic biorefineries: Perspectives for biopolymer production
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High-throughput interaction screens illuminate the role of c-di-AMP in cyanobacterial nighttime survival
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Photosynthetic generation of heterologous terpenoids in cyanobacteria
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Metabolic pathway engineering: Perspectives and applications
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Analysis of the light intensity dependence of the growth of Synechocystis and of the light distribution in a photobioreactor energized by 635 nm light
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“Genetic engineering of cyanobacteria has demonstrated the opportunity to channel solar energy into the formation of various commodity products ( Angermayr, Gorchs Rovira & Hellingwerf, 2015 ; Zhou et al., 2016 ).”
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Physical, chemical, and metabolic state sensors expand the synthetic biology toolbox for Synechocystis sp. PCC 6803
Immethun, C. M., DeLorenzo, D. M., Focht, C. M., Gupta, D. K., Johnson, C. B., & Moon, T. S. (2017) · Biotechnology and Bioengineering · doi:10.1002/bit.26275 · Cited by 45 · see citing works
Metabolic engineering of Synechococcus elongatus PCC 7942 for improvement of 1,3-propanediol and glycerol production based on in silico simulation of metabolic flux distribution
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Metabolic engineering of Synechocystis sp. PCC 6803 for the photoproduction of the sesquiterpene valencene
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Sustainable protein production through genetic engineering of cyanobacteria and use of atmospheric N2 gas
Nawaz, T., Gu, L., Fahad, S., Saud, S., Harrison, M. T., & Zhou, R. (2024) · Food and Energy Security · doi:10.1002/fes3.536 · Cited by 45 · see citing works
Utilising the native plasmid, pCA2.4, from the cyanobacterium Synechocystis sp. strain PCC6803 as a cloning site for enhanced product production
Armshaw, P., Carey, D., Sheahan, C., & Pembroke, J. T. (2015) · Biotechnology for Biofuels · doi:10.1186/s13068-015-0385-x · Cited by 44 · see citing works
Heterologous synthesis of geranyllinalool, a diterpenol plant product, in the cyanobacterium Synechocystis
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Heterocyst Thylakoid Bioenergetics
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The Fluctuating Cell-Specific Light Environment and Its Effects on Cyanobacterial Physiology
Andersson, B., Shen, C., Cantrell, M., Dandy, D. S., & Peers, G. (2019) · PLANT PHYSIOLOGY · doi:10.1104/pp.19.00480 · Cited by 38 · see citing works
Photonfluxostat: A method for light-limited batch cultivation of cyanobacteria at different, yet constant, growth rates
Du, W., Jongbloets, J. A., Hernández, H. P., Bruggeman, F. J., Hellingwerf, K. J., & dos Santos, F. B. (2016) · Algal Research · doi:10.1016/j.algal.2016.10.004 · Cited by 43 · see citing works
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Transomics data-driven, ensemble kinetic modeling for system-level understanding and engineering of the cyanobacteria central metabolism
Nishiguchi, H., Hiasa, N., Uebayashi, K., Liao, J. C., Shimizu, H., & Matsuda, F. (2019) · Metabolic Engineering · doi:10.1016/j.ymben.2019.01.004 · Cited by 43 · see citing works
Malic Enzyme Facilitates d -Lactate Production through Increased Pyruvate Supply during Anoxic Dark Fermentation in Synechocystis sp. PCC 6803
Hidese, R., Matsuda, M., Osanai, T., Hasunuma, T., & Kondo, A. (2020) · ACS Synthetic Biology · doi:10.1021/acssynbio.9b00281 · Cited by 43 · see citing works
Photosynthetic Conversion of Carbon Dioxide to Oleochemicals by Cyanobacteria: Recent Advances and Future Perspectives
Wang, L., Chen, L., Yang, S., & Tan, X. (2020) · Frontiers in Microbiology · doi:10.3389/fmicb.2020.00634 · Cited by 42 · see citing works
Alignment of microbial fitness with engineered product formation: obligatory coupling between acetate production and photoautotrophic growth
Du, W., Jongbloets, J. A., van Boxtel, C., Hernández, H. P., Lips, D., Olivier, B. G., Hellingwerf, K. J., & dos Santos, F. B. (2018) · Biotechnology for Biofuels · doi:10.1186/s13068-018-1037-8 · Cited by 42 · see citing works
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Remodeling of the Photosynthetic Chain Promotes Direct CO2 Conversion into Valuable Aromatic Compounds
Ni, J., Liu, H., Tao, F., Wu, Y., & Xu, P. (2018) · Angewandte Chemie International Edition · doi:10.1002/anie.201808402 · Cited by 42 · see citing works
An Improved Natural Transformation Protocol for the Cyanobacterium Synechocystis sp. PCC 6803
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A roadmap for the synthesis of separation networks for the recovery of bio-based chemicals: Matching biological and process feasibility
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Genetic engineering of Synechocystis PCC6803 for the photoautotrophic production of the sweetener erythritol
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Techno-economic analysis of a conceptual biofuel production process from bioethylene produced by photosynthetic recombinant cyanobacteria
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CO2 to succinic acid – Estimating the potential of biocatalytic routes
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Introduction of a green algal squalene synthase enhances squalene accumulation in a strain of Synechocystis sp. PCC 6803
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Authors: Angermayr, S. A., Gorchs Rovira, A., & Hellingwerf, K. J.
Affiliations: University of Amsterdam (NL)
Corresponding author: Hellingwerf, Klaas J.
ORCID: Angermayr, S. Andreas
Hellingwerf, Klaas J.
Venue: Trends in Biotechnology
Publisher: Elsevier BV
Published: 2015-06
Volume: 33
Issue: 6
Pages: 352-361
ISSN: 0167-7799 (ISSN-L)
DOI: 10.1016/j.tibtech.2015.03.009
OpenAlex: W2134069782
Cited by: 273
References: 94
Citations by year: 2026: 9 · 2025: 16 · 2024: 10 · 2023: 10 · 2022: 30 · 2021: 22 · 2020: 30 · 2019: 37 · 2018: 38 · 2017: 30 · 2016: 33 · 2015: 8
FWCI: 3.52
Type: journal article
Language: en
Open access: closed
Licence: www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations: Trends in biotechnology (publishedVersion)
PubMed (publishedVersion)
UvA-DARE (University of Amsterdam) (publishedVersion)
UvA-DARE (University of Amsterdam) (submittedVersion)
Data Archiving and Networked Services (DANS) (submittedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Photosynthetic Processes and Mechanisms
Keywords: cyanobacteria, metabolic engineering, cell factories, biofuels, bioplastics, commodity chemicals, photosynthetic carbon fixation, oxygenic photosynthesis, metabolic pathway engineering, product titer
MeSH terms: Cyanobacteria, Biotechnology, Carbon Dioxide, Humans, Light, Photosynthesis, Plastics, Biomass, Biofuels, Synthetic Biology, Metabolic Engineering

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Metabolic engineering of cyanobacteria for the synthesis of commodity products

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