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In vitro and in vivo growth suppression of human papillomavirus 16-positive cervical cancer cells by e6 siRNA

Yoshinouchi, Mitsuo, Yamada, Taketo, Kizaki, Masahiro, Fen, Jin, Koseki, Takeyoshi, Ikeda, Yasuo, Nishihara, Tatsuji, Yamato, Kenji·2003·Molecular Therapy
DOI 10.1016/j.ymthe.2003.08.004
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Abstract

Human papillomavirus type 16 (HPV16), a causative agent of cervical cancers, encodes the E6 and E7 oncogenes, whose simultaneous expression is pivotal for malignant transformation and maintenance of malignant phenotypes. In the hope of developing a gene-specific therapy for HPV-related cancer, we examined the effects of E6 short-interfering RNA (siRNA) on the expression of these oncogenes and on the cell growth of HPV16-related cervical cancer cells. Using SiHa cervical cancer cells, we demonstrated that E6 siRNA decreased the levels of mRNA encoding E6 as well as that encoding E7 protein and also induced nuclear accumulation of p53, the most important target of E6. E6 siRNA suppressed monolayer and anchorage-independent growth of SiHa cells, which was associated with p21(CIP1/WAF1) induction and hypophosphorylation of retinoblastoma protein. Further, SiHa cells treated with E6 siRNA formed tumors in NOD/SCID mice that were significantly smaller than in those treated with control siRNA. Our results show HPV E6 siRNA as a candidate for gene-specific therapy for HPV-related cervical cancer.

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Research works printed but not linked: 49

  1. de Villiers, 1989, Heterogeneity of the human papillomavirus group, J. Virol., 63, 4898, 10.1128/JVI.63.11.4898-4903.1989 · DOI
  2. Bosch, 1995, Prevalence of human papillomavirus in cervical cancer, J. Natl. Cancer Inst., 87, 796, 10.1093/jnci/87.11.796 · DOI
  3. Huibregtse, 1993, Localization of the E6-AP regions that direct human papillomavirus E6 binding, association with p53, and ubiquitination of associated proteins, Mol. Cell. Biol., 13, 4918, 10.1128/MCB.13.8.4918 · DOI · Paper page
  4. Huibregtse, 1993, Cloning and expression of the cDNA for E6-AP, a protein that mediates the interaction of the human papillomavirus E6 oncoprotein with p53, Mol. Cell. Biol., 13, 775, 10.1128/MCB.13.2.775 · DOI · Paper page
  5. Patel, 1999, The E6 protein of human papillomavirus type 16 binds to and inhibits co-activation by CBP and p300, EMBO J., 18, 5061, 10.1093/emboj/18.18.5061 · DOI · Paper page
  6. Lee, 1997, Binding of human virus oncoproteins to hDlg/SAP97, a mammalian homolog of the Drosophila discs large tumor suppressor protein, Proc. Natl. Acad. Sci. USA, 94, 6670, 10.1073/pnas.94.13.6670 · DOI · Paper page
  7. Kiyono, 1997, Binding of high-risk human papillomavirus E6 oncoproteins to the human homologue of the Drosophila discs large tumor suppressor protein, Proc. Natl. Acad. Sci. USA, 94, 11612, 10.1073/pnas.94.21.11612 · DOI · Paper page
  8. Klingelhutz, 1996, Telomerase activation by the E6 gene product of human papillomavirus type 16, Nature, 380, 79, 10.1038/380079a0 · DOI · Paper page
  9. Veldman, 2001, Transcriptional activation of the telomerase hTERT gene by human papillomavirus type 16 E6 oncoprotein, J. Virol., 75, 4467, 10.1128/JVI.75.9.4467-4472.2001 · DOI · Paper page
  10. Munger, 1989, Complex formation of human papillomavirus E7 proteins with the retinoblastoma tumor suppressor gene product, EMBO J., 8, 4099, 10.1002/j.1460-2075.1989.tb08594.x · DOI · Paper page
  11. Dyson, 1992, Homologous sequences in adenovirus E1A and human papillomavirus E7 proteins mediate interaction with the same set of cellular proteins, J. Virol., 66, 6893, 10.1128/JVI.66.12.6893-6902.1992 · DOI · Paper page
  12. Davies, 1993, Human papillomavirus type 16 E7 associates with a histone H1 kinase and with p107 through sequences necessary for transformation, J. Virol., 67, 2521, 10.1128/JVI.67.5.2521-2528.1993 · DOI
  13. Funk, 1997, Inhibition of CDK activity and PCNA-dependent DNA replication by p21 is blocked by interaction with the HPV-16 E7 oncoprotein, Genes Dev., 11, 2090, 10.1101/gad.11.16.2090 · DOI · Paper page
  14. Jones, 1997, The human papillomavirus E7 oncoprotein can uncouple cellular differentiation and proliferation in human keratinocytes by abrogating p21Cip1-mediated inhibition of cdk2, Genes Dev., 11, 2101, 10.1101/gad.11.16.2101 · DOI · Paper page
  15. Zerfass-Thome, 1996, Inactivation of the cdk inhibitor p27KIP1 by the human papillomavirus type 16 E7 oncoprotein, Oncogene, 13, 2323
  16. Fire, 1999, RNA-triggered gene silencing, Trends Genet., 15, 358, 10.1016/S0168-9525(99)01818-1 · DOI
  17. Bass, 2000, Double-stranded RNA as a template for gene silencing, Cell, 101, 235, 10.1016/S0092-8674(02)71133-1 · DOI
  18. Cogoni, 2000, Post-transcriptional gene silencing across kingdoms, Curr. Opin. Genet. Dev., 10, 638, 10.1016/S0959-437X(00)00134-9 · DOI
  19. Plasterk, 2000, The silence of the genes, Curr. Opin. Genet. Dev., 10, 562, 10.1016/S0959-437X(00)00128-3 · DOI
  20. Sijen, 2000, Post-transcriptional gene-silencing, Bioessays, 22, 520, 10.1002/(SICI)1521-1878(200006)22:6<520::AID-BIES5>3.0.CO;2-W · DOI
  21. Sharp, 2001, RNA interference—2001, Genes Dev., 15, 485, 10.1101/gad.880001 · DOI
  22. Hammond, 2001, Post-transcriptional gene silencing by double-stranded RNA, Nat. Rev. Genet., 2, 110, 10.1038/35052556 · DOI
  23. Tuschl, 2001, RNA interference and small interfering RNAs, ChemBioChem, 2, 239, 10.1002/1439-7633(20010401)2:4<239::AID-CBIC239>3.0.CO;2-R · DOI
  24. Elbashir, 2001, Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells, Nature, 411, 494, 10.1038/35078107 · DOI · Paper page
  25. Harborth, 2001, Identification of essential genes in cultured mammalian cells using small interfering RNAs, J. Cell Sci., 114, 4557, 10.1242/jcs.114.24.4557 · DOI
  26. Schwarz, 1985, Structure and transcription of human papillomavirus sequences in cervical carcinoma cells, Nature, 314, 111, 10.1038/314111a0 · DOI
  27. Freedman, 1998, Nuclear export is required for degradation of endogenous p53 by MDM2 and human papillomavirus E6, Mol. Cell. Biol., 18, 7288, 10.1128/MCB.18.12.7288 · DOI
  28. Mantovani, 1999, Inhibition of E6 induced degradation of p53 is not sufficient for stabilization of p53 protein in cervical tumour derived cell lines, Oncogene, 18, 3309, 10.1038/sj.onc.1202688 · DOI · Paper page
  29. Steele, 1993, Effects of human papillomavirus type 18-specific antisense oligonucleotides on the transformed phenotype of human carcinoma cell lines, Cancer Res., 53, 2330
  30. Venturini, 1999, Kinetic selection of HPV 16 E6/E7-directed antisense nucleic acids, Nucleic Acids Res., 27, 1585, 10.1093/nar/27.7.1585 · DOI
  31. Alvarez-Salas, 1998, Inhibition of HPV-16 E6/E7 immortalization of normal keratinocytes by hairpin ribozymes, Proc. Natl. Acad. Sci. USA, 95, 1189, 10.1073/pnas.95.3.1189 · DOI
  32. Chen, 1996, Effects on tumor cells of ribozymes that cleave the RNA transcripts of human papillomavirus type 18, Cancer Gene Ther, 3, 18
  33. Scheffner, 1991, The state of the p53 and retinoblastoma genes in human cervical carcinoma cell lines, Proc. Natl. Acad. Sci. USA, 88, 5523, 10.1073/pnas.88.13.5523 · DOI
  34. El-Deiry, 1993, WAF1, a potential mediator of p53 tumor suppression, Cell, 75, 817, 10.1016/0092-8674(93)90500-P · DOI · Paper page
  35. Harper, 1993, The p21 Cdk-interacting protein Cip1 is a potent inhibitor of G1 cyclin-dependent kinases, Cell, 75, 805, 10.1016/0092-8674(93)90499-G · DOI · Paper page
  36. Gu, 1993, Inhibition of CDK2 activity in vivo by an associated 20K regulatory subunit, Nature, 366, 707, 10.1038/366707a0 · DOI
  37. Xiong, 1993, p21 is a universal inhibitor of cyclin kinases, Nature, 366, 701, 10.1038/366701a0 · DOI
  38. Jiang, 2002, Selective silencing of viral gene expression in HPV-positive human cervical carcinoma cells treated with siRNA, a primer of RNA interference, Oncogene, 21, 6041, 10.1038/sj.onc.1205878 · DOI
  39. Smotkin, 1989, Oncogenic and nononcogenic human genital papillomaviruses generate the E7 mRNA by different mechanisms, J. Virol., 63, 1441, 10.1128/JVI.63.3.1441-1447.1989 · DOI
  40. Sherman, 1992, Expression and splicing patterns of human papillomavirus type-16 mRNAs in pre-cancerous lesions and carcinomas of the cervix, in human keratinocytes immortalized by HPV 16, and in cell lines established from cervical cancers, Int. J. Cancer, 50, 356, 10.1002/ijc.2910500305 · DOI
  41. Stacey, 1995, Translation of the human papillomavirus type 16 E7 oncoprotein from bicistronic mRNA is independent of splicing events within the E6 open reading frame, J. Virol., 69, 7023, 10.1128/JVI.69.11.7023-7031.1995 · DOI
  42. Xia, 2002, siRNA-mediated gene silencing in vitro and in vivo, Nat. Biotechnol., 20, 1006, 10.1038/nbt739 · DOI
  43. Gao, 1995, Cationic liposome-mediated gene transfer, Gene Ther., 2, 710
  44. Clark, 2000, Studies of direct intratumoral gene transfer using cationic lipid-complexed plasmid DNA, Cancer Gene Ther., 7, 853, 10.1038/sj.cgt.7700184 · DOI
  45. Kaneda, 2002, Hemagglutinating virus of Japan (HVJ) envelope vector as a versatile gene delivery system, Mol. Ther., 6, 219, 10.1006/mthe.2002.0647 · DOI
  46. Lewis, 2002, Efficient delivery of siRNA for inhibition of gene expression in postnatal mice, Nat. Genet., 32, 107, 10.1038/ng944 · DOI
  47. Sorensen, 2003, Gene silencing by systemic delivery of synthetic siRNAs in adult mice, J. Mol. Biol., 327, 761, 10.1016/S0022-2836(03)00181-5 · DOI
  48. Kanda, 1988, Human papillomavirus type 16 open reading frame E7 encodes a transforming gene for rat 3Y1 cells, J. Virol., 62, 610, 10.1128/JVI.62.2.610-613.1988 · DOI · Paper page
  49. Yamato, 2000, Dissociation of bone morphogenetic protein-mediated growth arrest and apoptosis of mouse B cells by HPV-16 E6/E7, Exp. Cell Res., 257, 198, 10.1006/excr.2000.4876 · DOI

Cited by

Metadata

Authors
Yoshinouchi, M., Yamada, T., Kizaki, M., Fen, J., Koseki, T., Ikeda, Y., Nishihara, T., & Yamato, K.
Venue
Molecular Therapy
Publisher
Elsevier BV
Published
2003-11
Volume
8
Issue
5
Pages
762-768
ISSN
1525-0016
DOI
10.1016/j.ymthe.2003.08.004
Cited by
156
References
1
Language
en
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