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Cited by 57Malaria Research and ControlBiosensors and Analytical DetectionDigital Imaging for Blood Diseases

Comparison of a rapid field immunochromatographic test to expert microscopy for the detection of Plasmodium falciparum asexual parasitemia in Thailand

Wongsrichanalai, C, Chuanak, N, Tulyayon, S, Thanoosingha, N, Laoboonchai, A, Thimasarn, K, Brewer, T.G, Heppner, D.G·1999·Acta Tropica
DOI 10.1016/s0001-706x(99)00040-6
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Abstract

We assessed a rapid, Plasmodium falciparum histidine rich protein 2 (PfHRP2)-based immunochromatographic test (ICT Malaria Pf Test), for detection of asexual P. falciparum parasitemia in 551 subjects in three groups: (1) symptomatic patients self-referring for diagnosis, (2) villagers in a screening survey, and (3) patients recently treated for P. falciparum malaria. Expert light microscopy was the reference standard. ICT test performance was similar for diagnostic and screening modes. Four findings emerged: (1) test sensitivity correlated directly with parasite density, (2) test band intensity correlated directly with parasite density, (3) persistent test positivity after parasite clearance precludes its use for monitoring early therapeutic responses, and (4) a false negative test at 18,000 parasites/microl is unexplained. We conclude that a strong positive ICT test is highly predictive of falciparum asexual parasitemia for the diagnosis of new cases of falciparum malaria in Thailand, but a negative test result is inadequate to exclude parasitemia < 300/microl, and in some instances, even a higher parasitemia.

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Crossref · 10.1016/s0001-706x(99)00040-6

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  • These shortcomings hinder the use of existing RDTs for managing malaria and for monitoring treatment efficacy.

    “These shortcomings hinder the use of existing RDT for managing malaria, and also for monitoring treatment efficacy [23–25].”

    From Diagnosing malaria in pregnancy: An update (2012) · cites this paper as [25]

  • An important weakness of RDT-HRP2 tests is the prolonged half-life of HRP-2 antigen, which can be identified in plasma several weeks after parasite clearance and therefore cannot distinguish current from recent infection.

    “An important weakness of RDT-HRP2 tests is the prolonged half-life of the antigen. HRP-2 can be identified in plasma samples several weeks after parasite clearance, and therefore cannot be used to distinguish current from recent infection [23–25].”

    From Diagnosing malaria in pregnancy: An update (2012) · cites this paper as [25]

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Authors
Wongsrichanalai, C., Chuanak, N., Tulyayon, S., Thanoosingha, N., Laoboonchai, A., Thimasarn, K., Brewer, T. G., & Heppner, D. G.
Affiliations
  • Armed Forces Research Institute of Medical Science (TH)
  • Ministry of Public Health (TH)
Corresponding author
Wongsrichanalai, C
ORCID
Brewer, T.G
Venue
Acta Tropica
Publisher
Elsevier BV
Published
1999-10
Volume
73
Issue
3
Pages
263-273
ISSN
0001-706X (ISSN-L)
DOI
10.1016/s0001-706x(99)00040-6
OpenAlex
W1973784424
Cited by
57
References
34
Citations by year
2021: 1 · 2018: 1 · 2017: 1 · 2015: 1 · 2014: 1 · 2013: 1 · 2012: 2
FWCI
6.72
Type
journal article
Language
en
Open access
closed
Licence
Locations
  • Acta Tropica (publishedVersion)
  • PubMed (publishedVersion)
Funders
Ministry of Public Health
Topics
  • Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Malaria Research and Control
  • Physical Sciences › Engineering › Biomedical Engineering › Biosensors and Analytical Detection
  • Physical Sciences › Computer Science › Computer Vision and Pattern Recognition › Digital Imaging for Blood Diseases
Keywords
Plasmodium falciparum, rapid immunochromatographic test, HRP-2, malaria diagnosis, parasite density, test sensitivity, malaria screening, treatment response monitoring, Thailand
MeSH terms
Histidine-Rich Glycoprotein, Adult, Animals, Chromatography, Evaluation Studies as Topic, Female, Humans, Immunoassay, Male, Microscopy, Plasmodium falciparum, Proteins, Reagent Kits, Diagnostic, Thailand, Protozoan Proteins, Malaria, Falciparum, Parasitemia

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