Qualitative And Semiquantitative Polymerase Chain Reaction To Predict Plasmodium falciparum Treatment Failure
Abstract
Multidrug-resistant falciparum malaria is increasing in most malaria-endemic areas. Rapid methods for predicting treatment failure would aid management and control of drug-resistant infections. In this study, Plasmodium falciparum DNA clearance was examined by qualitative and semiquantitative polymerase chain reaction (PCR). Thai patients with acute falciparum malaria were prospectively followed by light microscopy and by PCR of P. falciparum DNA eluted from filter paper blood samples. A 206-bp P. falciparum sequence was amplified and detected radiometrically and by high-performance liquid chromatography. Clearance of P. falciparum DNA was significantly delayed in treatment failures compared with that in successfully treated patients (P = .02). Semiquantitative PCR levels did not drop to < 50% of pretreatment levels until day 3 or later in treatment failures compared with day 1 or earlier for successfully treated parasitemia-matched controls (P = .005). These results suggest that qualitative and semiquantitative PCR may be useful as a method for monitoring response to therapy.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The DNA detected by PCR may be from a viable asexual parasite, a gametocyte, or represent nonviable DNA from past infections following treatment.
“The DNA detected by PCR may be from a viable asexual parasite, a gametocyte, or represent nonviable DNA from past infections following treatment. 35”
From What is the burden of submicroscopic malaria in pregnancy in central India? (2015) · cites this paper as [35]
Cites
Avoiding false positives with PCR
Kwok, S., & Higuchi, R. (1989) · Nature · doi:10.1038/339237a0 · Cited by 3,493 · see citing works
Aurelius, E., Johansson, B., Staland, Å., Forsgren, M., Aurelius, E., & Sköldenberg, B. (1991) · The Lancet · doi:10.1016/0140-6736(91)92155-u · Cited by 545 · see citing works
Detection of Plasmodium falciparum by Polymerase Chain Reaction in a Field Study
Sethabutr, O., Brown, A. E., Panyim, S., Kain, K. C., Webster, H. K., & Echeverria, P. D. (1992) · The Journal of Infectious Diseases · doi:10.1093/infdis/166.1.145 · Cited by 117 · see citing works
Self-citationA novel detection of a single plasmodium falciparum in infected blood
Tirasophon, W., Ponglikitmongkol, M., Wilairat, P., et al. (1991) · Biochemical and Biophysical Research Communications · doi:10.1016/s0006-291x(05)81217-3 · Cited by 61 · see citing works
Katz, E. D., Haff, L. A., & Eksteen, R. (1990) · Journal of Chromatography A · doi:10.1016/s0021-9673(01)89509-1 · Cited by 48 · see citing works
Rapid analysis and quantitation of PCR products by high-performance liquid chromatography.
Warren, W., Wheat, T. E., & Knudsen, P. (1991) · PubMed · Cited by 31 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Real-Time PCR for Detection and Identification of <i>Plasmodium</i> spp
Mangold, K. A., Manson, R. U., Koay, E. S. C., Stephens, L., Regner, M., Thomson, R. B., Peterson, L. R., & Kaul, K. L. (2005) · Journal of Clinical Microbiology · doi:10.1128/jcm.43.5.2435-2440.2005 · Cited by 278 · see citing works
Batwala, V., Magnussen, P., & Nuwaha, F. (2010) · Malaria Journal · doi:10.1186/1475-2875-9-349 · Cited by 101 · see citing works
Detection and clinical manifestation of placental malaria in southern Ghana
Mockenhaupt, F. P., Bedu-Addo, G., von Gaertner, C., Boyé, R., Fricke, K., Hannibal, I., Karakaya, F., Schaller, M., Ulmen, U., Acquah, P. A., et al. (2006) · Malaria Journal · doi:10.1186/1475-2875-5-119 · Cited by 81 · see citing works
EVALUATION OF A MALARIA RAPID DIAGNOSTIC TEST FOR ASSESSING THE BURDEN OF MALARIA DURING PREGNANCY
SINGER, L. M., NEWMAN, R. D., DIARRA, A., MORAN, A. C., HUBER, C. S., STENNIES, G., SIRIMA, S. B., KONATE, A., YAMEOGO, M., SAWADOGO, R., et al. (2004) · The American Journal of Tropical Medicine and Hygiene · doi:10.4269/ajtmh.2004.70.481 · Cited by 77 · see citing works
Wongsrichanalai, C., Chuanak, N., Tulyayon, S., Thanoosingha, N., Laoboonchai, A., Thimasarn, K., Brewer, T. G., & Heppner, D. G. (1999) · Acta Tropica · doi:10.1016/s0001-706x(99)00040-6 · Cited by 57 · see citing works
Self-citationWhat is the burden of submicroscopic malaria in pregnancy in central India?
Singh, N., Bharti, P. K., Singh, M. P., Singh, R., Yeboah-Antwi, K., Desai, M., Udhayakumar, V., Muniyandi, M., Hamer, D. H., & Wylie, B. J. (2015) · Pathogens and Global Health · doi:10.1179/2047773215y.0000000002 · Cited by 25 · see citing works
- Authors
- Kain, K. C., Kyle, D. E., Wongsrichanalai, C., Brown, A. E., Webster, H. K., Vanijanonta, S., & Looareesuwan, S.
- Affiliations
- University of Toronto (CA)
- Venue
- Journal of Infectious Diseases
- Publisher
- Oxford University Press (OUP)
- Published
- 1994-12-01
- Volume
- 170
- Issue
- 6
- Pages
- 1626-1630
- ISSN
- 0022-1899 (ISSN-L)
- 1537-6613
- OpenAlex
- W2111340611
- Cited by
- 43
- References
- 6
- Citations by year
- 2021: 1 · 2020: 1 · 2019: 1 · 2015: 5 · 2014: 1
- FWCI
- 1.54
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- The Journal of Infectious Diseases (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Malaria Research and Control
- Health Sciences › Medicine › Genetics › Hemoglobinopathies and Related Disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Phytochemical compounds biological activities
- Keywords
- Plasmodium falciparum, falciparum malaria, multidrug resistance, treatment failure prediction, semi-quantitative PCR, qualitative PCR, filter paper blood samples, radiometric detection, HPLC
- MeSH terms
- Adolescent, Adult, Animals, Antimalarials, Base Sequence, Chromatography, High Pressure Liquid, Drug Resistance, Female, Humans, Male, Molecular Sequence Data, Plasmodium falciparum, Prospective Studies, Recurrence, Sensitivity and Specificity, Thailand, DNA, Protozoan, Polymerase Chain Reaction, Malaria, Falciparum, Treatment Failure, Parasitemia
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
