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Cited by 43Aluminum toxicity and tolerance in plants and animalsErythrocyte Function and PathophysiologyHeavy Metal Exposure and Toxicity

The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.

Human erythroid cells are affected by aluminium. Alteration of membrane band 3 protein

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Vittori, Daniela, Garbossa, Graciela, Lafourcade, Carlos, Pérez, Gladys, Nesse, Alcira·2002·Biochimica et Biophysica Acta (BBA) - Biomembranes
DOI 10.1016/s0005-2736(01)00427-8
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Abstract

There is evidence that anaemia is associated with aluminium (Al). We have already reported on the sensitivity to Al, showed by erythroid cell populations of animals chronically exposed to the metal. In order to investigate whether Al could also affect human cells, experiments were carried out both on immature and mature human erythroid cells. Erythroid progenitors (CFU-E, colony-forming units-erythroid) concentrated from human peripheral blood were cultured in an Al-rich medium under erythropoietin stimulation and their development analysed. Human peripheral erythrocytes were aged in the presence of Al. Cells were examined using scanning electron microscopy, and membrane proteins analysed by polyacrylamide gel electrophoresis with sodium dodecyl sulphate and immunoblotting. The development of the Al-treated progenitors was 8750/6600-9200 CFU-E/10(6) cells, a significantly lower median value (P<0.05) than that showed by non-treated cells (12300/11200-20700 CFU-E/10(6) cells). Erythrocyte morphological changes were induced by Al during the in vitro ageing. The cells lost their typical biconcave shape, turning into acanthocytes and stomatocytes. Simultaneously, an increased membrane protein breakdown compatible with band 3 degradation was detected. Besides, Al was found within the cells and attached to the membrane. The present in vitro results suggest that Al may disturb human erythropoiesis through combined effects on mature erythrocytes and cellular metabolism in late erythroid progenitors.

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Crossref · 10.1016/s0005-2736(01)00427-8

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  • Al-induced change in erythrocyte size may be accompanied by change in erythrocyte shape resulting in formation of echinocytes, acanthocytes and stomatocytes in vitro due to altered membrane morphology.

    “The Al-induced change in erythrocyte size may also be accompanied by change in erythrocyte shape resulting in the formation of echinocytes (Suwalsky et al., 2004), acanthocytes and stomatocytes (Vittori et al., 2002) in vitro, due to altered membrane morphology (Lukyanenko et al., 2013).”

    From Aluminium toxicosis: a review of toxic actions and effects (2019) · cites this paper as Vittori et al., 2002

  • The effect of Al on erythroid progenitor cells and erythrocytes was associated with slow growth and increased degradation of membrane band 3 proteins, respectively.

    “The effect of Al on erythroid progenitor cells and erythrocytes was associated with slow growth and increased degradation of membrane band 3 proteins, respectively (Vittori et al., 2002).”

    From Aluminium toxicosis: a review of toxic actions and effects (2019) · cites this paper as Vittori et al., 2002

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Metadata

Authors
Vittori, D., Garbossa, G., Lafourcade, C., Pérez, G., & Nesse, A.
Affiliations
Universidad de Buenos Aires (AR)
Corresponding author
Vittori, Daniela
ORCID
Venue
Biochimica et Biophysica Acta (BBA) - Biomembranes
Publisher
Elsevier BV
Published
2002-02
Volume
1558
Issue
2
Pages
142-150
ISSN
0005-2736 (ISSN-L)
DOI
10.1016/s0005-2736(01)00427-8
OpenAlex
W1990300107
Cited by
43
References
1
Citations by year
2026: 1 · 2024: 2 · 2023: 3 · 2022: 4 · 2020: 4 · 2019: 1 · 2018: 1 · 2016: 1 · 2015: 3 · 2014: 1 · 2013: 2 · 2012: 6
FWCI
2.53
Type
journal article
Language
en
Open access
Licence
Locations
  • Biochimica et Biophysica Acta (BBA) - Biomembranes (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Consejo Nacional de Investigaciones Científicas y Técnicas
  • Universidad de Buenos Aires
  • Agencia Nacional de Promoción Científica y Tecnológica
Topics
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Aluminum toxicity and tolerance in plants and animals
  • Health Sciences › Medicine › Physiology (medical) › Erythrocyte Function and Pathophysiology
  • Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Heavy Metal Exposure and Toxicity
Keywords
aluminum toxicity, erythroid cells, band 3 protein, erythropoiesis, erythrocyte aging, erythrocyte morphology, acanthocytes, stomatocytes, erythroid progenitor cells, CFU-E, anemia, membrane protein degradation
MeSH terms
Anion Exchange Protein 1, Erythrocyte, Cytosol, Erythrocyte Aging, Erythrocyte Membrane, Erythrocytes, Humans, Time Factors, Immunoblotting, Erythroid Precursor Cells, Aluminum Compounds

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