Vitamin C supplementation in normal subjects reduces constitutive ICAM-1 expression
Abstract
Regulation of monocyte adhesion molecule gene expression is via redox sensitive transcription factors. We have investigated whether dietary antioxidant supplementation with vitamin C (250 mg/day) can modulate monocyte ICAM-1 expression in healthy male subjects with low plasma vitamin C at baseline. In a randomised, double-blind, crossover study, monocyte ICAM-1 mRNA was analysed using quantitative reverse transcriptase PCR. Protein was determined by flow cytometry (monocytes) and ELISA (plasma). Monocyte numbers were unaltered by supplementation. Subjects with low plasma vitamin C (<50 microM) prior to supplementation expressed higher levels of monocyte ICAM-1mRNA, and showed a significant (50%) reduction in ICAM-1mRNA expression after 6 weeks of 250 mg/day vitamin C supplementation (p<0.05). This was paralleled by a reduction in sICAM-1 (p<0.05). For the first time, these results show that dietary vitamin C can modulate monocyte ICAM-1 gene expression in vivo, where regulation of gene expression represents a novel mechanism for benefit from dietary antioxidants.
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Apoptosis mediated by inflammatory cytokines and oxidized low-density lipoproteins can be attenuated by vitamin C.
“Apoptosis mediated by inflammatory cytokines and oxidized low-density lipoproteins (LDL) can be attenuated by vitamin C (Rayment et al. 2003).”
From Reactive Oxygen Species, Toxicity, Oxidative Stress, and Antioxidants: Chronic Diseases and Aging (2023) · cites this paper as Rayment et al. 2003
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- Authors
- Rayment, S. J., Shaw, J., Woollard, K. J., Lunec, J., & Griffiths, H. R.
- Affiliations
- Aston University (GB)
- University of Leicester (GB)
- Corresponding author
- Griffiths, Helen R
- Venue
- Biochemical and Biophysical Research Communications
- Publisher
- Elsevier BV
- Published
- 2003-08
- Volume
- 308
- Issue
- 2
- Pages
- 339-345
- ISSN
- 0006-291X (ISSN-L)
- OpenAlex
- W2011224043
- Cited by
- 45
- References
- 38
- Citations by year
- 2023: 1 · 2022: 2 · 2021: 3 · 2020: 1 · 2019: 1 · 2018: 1 · 2017: 1 · 2016: 1 · 2015: 2 · 2014: 3 · 2013: 2 · 2012: 4
- FWCI
- 2.86
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biochemical and Biophysical Research Communications (publishedVersion)
- PubMed (publishedVersion)
- Aston Publications Explorer (Aston University) (submittedVersion)
- Funders
- Food Standards Agency
- Topics
- Health Sciences › Nursing › Nutrition and Dietetics › Vitamin C and Antioxidants Research
- Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
- Health Sciences › Medicine › Rehabilitation › Exercise and Physiological Responses
- Keywords
- vitamin C supplementation, ICAM-1, gene expression regulation, dietary antioxidants, sICAM-1, plasma vitamin C, redox-sensitive transcription factors, monocytes
- MeSH terms
- Adult, Antioxidants, Ascorbic Acid, Base Sequence, Double-Blind Method, Humans, Male, Middle Aged, Monocytes, RNA, Messenger, Gene Expression, Cross-Over Studies, Intercellular Adhesion Molecule-1
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