Cited by 45Vitamin C and Antioxidants ResearchAntioxidant Activity and Oxidative StressExercise and Physiological Responses

Vitamin C supplementation in normal subjects reduces constitutive ICAM-1 expression

Rayment, Sarah J, Shaw, Jacqui, Woollard, Kevin J, Lunec, Joe, Griffiths, Helen R·2003·Biochemical and Biophysical Research Communications

Abstract

Regulation of monocyte adhesion molecule gene expression is via redox sensitive transcription factors. We have investigated whether dietary antioxidant supplementation with vitamin C (250 mg/day) can modulate monocyte ICAM-1 expression in healthy male subjects with low plasma vitamin C at baseline. In a randomised, double-blind, crossover study, monocyte ICAM-1 mRNA was analysed using quantitative reverse transcriptase PCR. Protein was determined by flow cytometry (monocytes) and ELISA (plasma). Monocyte numbers were unaltered by supplementation. Subjects with low plasma vitamin C (<50 microM) prior to supplementation expressed higher levels of monocyte ICAM-1mRNA, and showed a significant (50%) reduction in ICAM-1mRNA expression after 6 weeks of 250 mg/day vitamin C supplementation (p<0.05). This was paralleled by a reduction in sICAM-1 (p<0.05). For the first time, these results show that dietary vitamin C can modulate monocyte ICAM-1 gene expression in vivo, where regulation of gene expression represents a novel mechanism for benefit from dietary antioxidants.

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Crossref · 10.1016/s0006-291x(03)01383-4

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Authors
Rayment, S. J., Shaw, J., Woollard, K. J., Lunec, J., & Griffiths, H. R.
Affiliations
  • Aston University (GB)
  • University of Leicester (GB)
Corresponding author
Griffiths, Helen R
Venue
Biochemical and Biophysical Research Communications
Publisher
Elsevier BV
Published
2003-08
Volume
308
Issue
2
Pages
339-345
ISSN
0006-291X (ISSN-L)
OpenAlex
W2011224043
Cited by
45
References
38
Citations by year
2023: 1 · 2022: 2 · 2021: 3 · 2020: 1 · 2019: 1 · 2018: 1 · 2017: 1 · 2016: 1 · 2015: 2 · 2014: 3 · 2013: 2 · 2012: 4
FWCI
2.86
Type
journal article
Language
en
Open access
closed
Locations
  • Biochemical and Biophysical Research Communications (publishedVersion)
  • PubMed (publishedVersion)
  • Aston Publications Explorer (Aston University) (submittedVersion)
Funders
Food Standards Agency
Topics
  • Health Sciences › Nursing › Nutrition and Dietetics › Vitamin C and Antioxidants Research
  • Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
  • Health Sciences › Medicine › Rehabilitation › Exercise and Physiological Responses
Keywords
vitamin C supplementation, ICAM-1, gene expression regulation, dietary antioxidants, sICAM-1, plasma vitamin C, redox-sensitive transcription factors, monocytes
MeSH terms
Adult, Antioxidants, Ascorbic Acid, Base Sequence, Double-Blind Method, Humans, Male, Middle Aged, Monocytes, RNA, Messenger, Gene Expression, Cross-Over Studies, Intercellular Adhesion Molecule-1

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