Cited by 48Neurotransmitter Receptor Influence on BehaviorNeuroscience and Neuropharmacology ResearchParkinson's Disease Mechanisms and Treatments

Viral restoration of dopamine to the nucleus accumbens is sufficient to induce a locomotor response to amphetamine

Heusner, Carrie L., Hnasko, Thomas S., Szczypka, Mark S., Liu, Yuhong, During, Matthew J., Palmiter, Richard D.·2003·Brain Research

Abstract

Administration of amphetamine to mice evokes hyperlocomotion. Dopamine deficient (DD) mice, in which tyrosine hydroxylase (TH) has been specifically inactivated in dopaminergic neurons, have a blunted response to amphetamine, indicating that the hyperlocomotive response requires dopamine. Dopamine production can be restored to specific brain regions by using adeno-associated viruses expressing TH and GTP cyclohydrolase 1 (GTPCH1). Restoration of dopamine specifically to the nucleus accumbens (NAc) of DD mice completely restores the ability of these mice to respond to amphetamine. This response is specific to the dopamine production in the NAc, as restoration of dopamine production to the caudate putamen (CPu) does not fully restore the hyperlocomotive response to amphetamine. These data support previous studies in which accumbal dopamine is required for producing a normal locomotor response to amphetamine and further show that release of dopamine restricted to the NAc is sufficient for this response

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Crossref · 10.1016/s0006-8993(03)02986-x

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Authors
Heusner, C. L., Hnasko, T. S., Szczypka, M. S., Liu, Y., During, M. J., & Palmiter, R. D.
Affiliations
  • University of Washington (US)
  • Thomas Jefferson University (US)
  • University of Auckland (NZ)
  • Howard Hughes Medical Institute (US)
Corresponding author
Palmiter, Richard D.
Venue
Brain Research
Publisher
Elsevier BV
Published
2003-08
Volume
980
Issue
2
Pages
266-274
ISSN
0006-8993 (ISSN-L)
OpenAlex
W2095555089
Cited by
48
References
31
Citations by year
2026: 1 · 2024: 1 · 2021: 1 · 2020: 3 · 2019: 1 · 2018: 2 · 2017: 2 · 2016: 2 · 2015: 1 · 2014: 1 · 2013: 2 · 2012: 1
FWCI
2.08
Type
journal article
Language
en
Open access
closed
Locations
  • Brain Research (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health (2t32da007278-31)
  • National Institute on Drug Abuse (T32-DA07278)
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurotransmitter Receptor Influence on Behavior
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Health Sciences › Medicine › Neurology › Parkinson's Disease Mechanisms and Treatments
Keywords
amphetamine, hyperlocomotion, dopamine, nucleus accumbens, caudate-putamen, tyrosine hydroxylase, GTP cyclohydrolase I, adeno-associated virus, gene therapy, dopamine deficiency, dopaminergic neurons, locomotor response
MeSH terms
Dependovirus, Amphetamine, Animals, Dopamine, Genetic Vectors, Humans, Motor Activity, Nucleus Accumbens, Recombination, Genetic, Genetic Therapy, Mice

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