The DOI matches its Crossref record; 31 of the 31 references it cites were found in a registry or an index.
Ser727-dependent transcriptional activation by association of p300 with STAT3 upon IL-6 stimulation
Open accessAbstract
Activation of the signal transducer and activator of transcription 3 (STAT3) in response to interleukin-6 (IL-6) type cytokines involves both phosphorylation of Tyr705, which enables dimerization, nuclear translocation and DNA binding, as well as ser727 phosphorylation. Here, we describe that the 65 C-terminal amino acids of STAT3 can function as an independent transcription activation domain (TAD), particularly when a negative charge is introduced at position 727 by mutation of the serine residue into aspartate. The strong transcriptional activity of the C-terminal STAT3 Ser727Asp TAD is coupled to a constitutive association with the co-activator p300. In HepG2 cells, p300 associates with STAT3 upon IL-6 stimulation, and overexpression of p300 enhances the transcriptional activity of STAT3alpha, but not of STAT3beta or STAT3 Ser727Ala. We conclude that Ser727 phosphorylation in the C-terminal region of STAT3 is required for transactivation by association with p300.
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“STAT3 transcriptional activity has been attributed to its transactivation domain that binds transcription co-activators such as p300 [46].”
From Selective STAT3-α or -β expression reveals spliceform-specific phosphorylation kinetics, nuclear retention and distinct gene expression outcomes (2012) · cites this paper as [46]
Citations
Cites
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TRANSCRIPTIONAL RESPONSES TO POLYPEPTIDE LIGANDS: The JAK-STAT Pathway
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Bromberg, J., & Darnell, J. E. (2000) · Oncogene · doi:10.1038/sj.onc.1203476 · Cited by 1,201
Serine phosphorylation of STATs
Decker, T., & Kovarik, P. (2000) · Oncogene · doi:10.1038/sj.onc.1203481 · Cited by 887
Schindler, C., Fu, X.-Y., Improta, T., Aebersold, R. H., & Darnell, J. E., Jr. (1992) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.89.16.7836 · Cited by 647
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Self-citationKadonaga, J. T. (1998) · Cell · doi:10.1016/s0092-8674(00)80924-1 · Cited by 554
Research works printed but not linked: 4
- Schuringa J.J. Timmer H. Luttickhuizen D. Vellenga E. and Kruijer W. (2001) Cytokine in press.
- Graham F.L., 1973, Mol. Cell Biol., 2, 607
- Bannister A.J., 1995, Oncogene, 11, 2509
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Cited by
Sorted by citations
Persistently Activated Stat3 Maintains Constitutive NF-κB Activity in Tumors
Lee, H., Herrmann, A., Deng, J.-H., Kujawski, M., Niu, G., Li, Z., Forman, S., Jove, R., Pardoll, D. M., & Yu, H. (2009) · Cancer Cell · doi:10.1016/j.ccr.2009.02.015 · Cited by 683
Interleukin-6: Biology, signaling and strategies of blockade
Schaper, F., & Rose‐John, S. (2015) · Cytokine & Growth Factor Reviews · doi:10.1016/j.cytogfr.2015.07.004 · Cited by 532
Therapeutically exploiting STAT3 activity in cancer — using tissue repair as a road map
Huynh, J., Chand, A. L., Gough, D. J., & Ernst, M. (2018) · Nature reviews. Cancer · doi:10.1038/s41568-018-0090-8 · Cited by 522
Lejeune, D., Dumoutier, L., Constantinescu, S. N., Kruijer, W., Schuringa, J. J., & Renauld, J. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m204204200 · Cited by 499
Self-citationGray, M. J., Zhang, J., Ellis, L. M., Semenza, G. L., Evans, D. B., Watowich, S. S., & Gallick, G. E. (2005) · Oncogene · doi:10.1038/sj.onc.1208513 · Cited by 378
Metadata
- Authors
- Schuringa, J.-J., Schepers, H., Vellenga, E., & Kruijer, W.
- Affiliations
- Sofia University "St. Kliment Ohridski" (BG)
- Venue
- FEBS Letters
- Publisher
- Wiley
- Published
- 2001-04-19
- Volume
- 495
- Issue
- 1-2
- Pages
- 71-76
- ISSN
- 0014-5793 (ISSN-L)
- 1873-3468
- OpenAlex
- W11322950
- Cited by
- 124
- References
- 31
- Citations by year
- 2026: 2 · 2025: 1 · 2024: 3 · 2023: 2 · 2022: 1 · 2021: 6 · 2020: 2 · 2019: 6 · 2018: 5 · 2017: 6 · 2016: 2 · 2015: 5 · 2014: 6 · 2013: 10 · 2012: 7
- FWCI
- 1.47
- Type
- journal article
- Language
- en
- Open access
- green
- doaj.org
- Licence
- Locations
- FEBS Letters (publishedVersion)
- PubMed (publishedVersion)
- Bollettino del CILEA (CILEA) (publishedVersion)
- University of Groningen research database (University of Groningen / Centre for Information Technology) (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- University of Groningen research database (University of Groningen / Centre for Information Technology) (submittedVersion)
- Hystrix (cc-by-nc)
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › Cytokine Signaling Pathways and Interactions
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › NF-κB Signaling Pathways
- Keywords
- STAT3, P300, interleukin-6, transactivation domain, transcriptional coactivator, STAT3β, cytokine signaling
- MeSH terms
- Animals, Cell Line, DNA-Binding Proteins, Carcinoma, Hepatocellular, Humans, Nuclear Proteins, Phosphorylation, Protein Binding, Serine, Transcriptional Activation, Trans-Activators, Interleukin-6, Mutagenesis, Site-Directed, Protein Structure, Tertiary, Amino Acid Substitution, STAT3 Transcription Factor
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