The DOI matches its Crossref record; 19 of the 19 references it cites were found in a registry or an index.
Generation of Arabidopsis thaliana plants with complex N‐glycans lacking β1,2‐linked xylose and core α1,3‐linked fucose
Abstract
The plant glycosyltransferases, β1,2‐xylosyltransferase (XylT) and core α1,3‐fucosyltransferase (FucT), are responsible for the transfer of β1,2‐linked xylose and core α1,3‐linked fucose residues to glycoprotein N‐glycans. These glycan epitopes are not present in humans and thus may cause immunological responses, which represent a limitation for the therapeutic use of recombinant mammalian glycoproteins produced in transgenic plants. Here we report the genetic modification of the N‐glycosylation pathway in Arabidopsis thaliana plants. Knockout plants were generated with complete deficiency of XylT and FucT. These plants lack antigenic protein‐bound N‐glycans and instead synthesise predominantly structures with two terminal β N‐acetylglucosamine residues (GlcNAc 2 Man 3 GlcNAc 2).
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Cites
Genome-Wide Insertional Mutagenesis of Arabidopsis thaliana
Alonso, J. M., Stepanova, A. N., Leisse, T. J., Kim, C. J., Chen, H., Shinn, P., Stevenson, D. K., Zimmerman, J., Barajas, P., Cheuk, R., et al. (2003) · Science · doi:10.1126/science.1086391 · Cited by 5,102
The production of recombinant pharmaceutical proteins in plants
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N-Glycoprotein biosynthesis in plants: recent developments and future trends
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Immunoglobulin E antibodies that crossreact with vegetable foods, pollen, and Hymenoptera venom
Aalberse, R. C., Koshte, V., & Clemens, J. G. J. (1981) · Journal of Allergy and Clinical Immunology · doi:10.1016/0091-6749(81)90133-0 · Cited by 409
van Ree, R., Cabanes-Macheteau, M., Akkerdaas, J., Milazzo, J.-P., Loutelier-Bourhis, C., Rayon, C., Villalba, M., Koppelman, S., Aalberse, R., Rodriguez, R., et al. (2000) · Journal of Biological Chemistry · doi:10.1074/jbc.275.15.11451 · Cited by 388
Galactose-extended glycans of antibodies produced by transgenic plants
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Function and glycosylation of plant-derived antiviral monoclonal antibody
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Cited by
Antibody therapeutics: isotype and glycoform selection
Jefferis, R. (2007) · Expert Opinion on Biological Therapy · doi:10.1517/14712598.7.9.1401 · Cited by 294
STRASSER, R., STADLMANN, J., SVOBODA, B., ALTMANN, F., GLÖSSL, J., & MACH, L. (2005) · Biochemical Journal · doi:10.1042/bj20041686 · Cited by 105
Self-citationKarg, S. R., Frey, A. D., Ferrara, C., Streich, D. K., Umaña, P., & Kallio, P. T. (2009) · Plant Physiology and Biochemistry · doi:10.1016/j.plaphy.2008.10.005 · Cited by 19
Metadata
- Authors
- Strasser, R., Altmann, F., Mach, L., Glössl, J., & Steinkellner, H.
- Venue
- FEBS Letters
- Publisher
- Wiley
- Published
- 2004-02-20
- Volume
- 561
- Issue
- 1-3
- Pages
- 132-136
- ISSN
- 0014-5793 (ISSN-L)
- 1873-3468
- OpenAlex
- W1966887838
- Cited by
- 321
- References
- 19
- FWCI
- 11.22
- Type
- journal article
- Language
- en
- Open access
- closed
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- Strasser, R., Altmann, F., Mach, L., Glössl, J., & Steinkellner, H. (2004). Generation of Arabidopsis thaliana plants with complex N‐glycans lacking β1,2‐linked xylose and core α1,3‐linked fucose. FEBS Letters. https://doi.org/10.1016/s0014-5793(04)00150-4
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