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Identification of a novel angiotensin‐I‐converting enzyme inhibitory peptide corresponding to a tryptic fragment of bovine β‐lactoglobulin
Open accessAbstract
The angiotensin‐I‐converting enzyme (ACE) inhibitory activity of a tryptic digest of bovine β‐lactoglobulin (β‐lg) was investigated. Intact β‐lg essentially did not inhibit ACE while the tryptic digest gave an 84.3% inhibition of ACE. Peptide material eluting between 20 and 25% acetonitrile during C 18 solid‐phase extraction of the β‐lg tryptic digest inhibited ACE by 93.6%. This solid‐phase extraction fraction was shown by mass spectroscopy to contain β‐lg f(142–148). This peptide had an ACE IC 50 value of 42.6 μmol/l. The peptide was resistant to further digestion with pepsin and was hydrolysed to a very low extent with chymotrypsin. The contribution of specific amino acid residues within the peptide to ACE inhibitory activity and the potential application of this peptide as a nutraceutical is discussed.
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Yamamoto, N., Akino, A., & Takano, T. (1994) · Journal of Dairy Science · doi:10.3168/jds.s0022-0302(94)77026-0 · Cited by 418
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Clare, D. A., & Swaisgood, H. E. (2000) · Journal of Dairy Science · doi:10.3168/jds.s0022-0302(00)74983-6 · Cited by 718
Bioactive peptides and proteins from foods: indication for health effects.
Möller, N. P., Scholz-Ahrens, K. E., Roos, N., & Schrezenmeir, J. (2008) · European Journal of Nutrition · doi:10.1007/s00394-008-0710-2 · Cited by 595
Bioactive peptides of animal origin: a review
Bhat, Z. F., Kumar, S., & Bhat, H. F. (2015) · Journal of Food Science and Technology · doi:10.1007/s13197-015-1731-5 · Cited by 308
Meisel, H., & Bockelmann, W. (1999) · Antonie van Leeuwenhoek · doi:10.1023/a:1002063805780 · Cited by 284
Self-citationAngiotensin I-converting-enzyme-inhibitory and antimicrobial bioactive peptides.
Gobbetti, M., Minervini, F., & Rizzello, C. G. (2004) · International Journal of Dairy Technology · doi:10.1111/j.1471-0307.2004.00139.x · Cited by 268
β-Lactoglobulin as source of bioactive peptides.
Hernández-Ledesma, B., Recio, I., & Amigo, L. (2008) · Amino Acids · doi:10.1007/s00726-007-0585-1 · Cited by 210
Antihypertensive Peptides Derived from Egg Proteins
Miguel, M., & Aleixandre, A. (2006) · The Journal of Nutrition · doi:10.1093/jn/136.6.1457 · Cited by 168
Ricci-Cabello, I., Olalla Herrera, M., & Artacho, R. (2012) · Nutrition Reviews · doi:10.1111/j.1753-4887.2011.00448.x · Cited by 142
Barla, F., Koyanagi, T., Tokuda, N., Matsui, H., Katayama, T., Kumagai, H., Michihata, T., Sasaki, T., Tsuji, A., & Enomoto, T. (2016) · Biotechnology Reports · doi:10.1016/j.btre.2016.04.002 · Cited by 51
Milk Derived Peptides with Immune Stimulating Antiviral Properties
Sun, H., & Jensse, H. (2012) · Milk Protein · doi:10.5772/50158 · Cited by 12
Metadata
- Authors
- Mullally, M. M., Meisel, H., & FitzGerald, R. J.
- Venue
- FEBS Letters
- Publisher
- Wiley
- Published
- 1997-01-27
- Volume
- 402
- Issue
- 2-3
- Pages
- 99-101
- ISSN
- 0014-5793 (ISSN-L)
- 1873-3468
- OpenAlex
- W2056357797
- Cited by
- 252
- References
- 1
- FWCI
- 1.57
- Type
- journal article
- Language
- en
- Open access
- bronze
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