CoChat
Sign Up
Cited by 79

The DOI matches its Crossref record.

Differing structural requirements for GTPase-activating protein responsiveness and NADPH oxidase activation by Rac.

Xu, X., Barry, D.C., Settleman, J., Schwartz, M.A., Bokoch, G.M.·1994·Journal of Biological Chemistry
DOI 10.1016/s0021-9258(17)31553-3
Open this paper in CoChat — free account

Abstract

The Rac GTP-binding proteins regulate the actin cytoskeleton and the superoxide-forming NADPH oxidase of phagocytic leukocytes. These functions of Rac are determined by the GTP/GDP state of the protein, which can be modulated by GTPase-activating proteins (GAPs). The interaction of Ras with both downstream signaling targets and GAPs is mediated via an "effector" domain (amino acids 30-40). We demonstrate that the effector domain of Rac2 is required for both NADPH oxidase activation and actin assembly, but that mutations in this region do not decrease the responsiveness of Rac to GAPs. In contrast, mutations of residues 12 (Gly-->Val) or 61 (Gln-->Leu) inhibit both intrinsic- and GAP-stimulated GTP hydrolysis by Rac2. A double mutation in which both the effector domain and Q61L were modified restored NADPH oxidase activation and membrane ruffling, while the equivalent effector domain and G12V double mutation did not. The Rac2 Q61L mutant had an increased "affinity" for NADPH oxidase activation and for GAP binding as compared to the wild type or G12V proteins. These experiments suggest that Rac contains at least two "effector" interaction sites, and that changes in binding interactions at one of these sites may influence the function of the other.

Overview

Integrity signals

Registry

Crossref · 10.1016/s0021-9258(17)31553-3

Cites

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Citations

Cites

No references could be resolved yet.

Research works printed but not linked: 36

  1. Hall, 1993, Curr. Opin. Cell Biol., 5, 265, 10.1016/0955-0674(93)90114-6 · DOI
  2. Bokoch, 1993, FASEB J., 7, 750, 10.1096/fasebj.7.9.8330683 · DOI
  3. Ridley, 1992, Cell, 70, 401, 10.1016/0092-8674(92)90164-8 · DOI
  4. Knaus, 1991, Science, 254, 1512, 10.1126/science.1660188 · DOI · Paper page
  5. Abo, 1991, Nature, 353, 668, 10.1038/353668a0 · DOI
  6. Bokoch, 1994, Curr. Opin. Immunol., 6, 98, 10.1016/0952-7915(94)90040-X · DOI
  7. Morel, 1993, Eur. J. Biochem., 201, 523, 10.1111/j.1432-1033.1991.tb16312.x · DOI
  8. Heyworth, 1993, Mol. Biol. Cell, 4, 261, 10.1091/mbc.4.3.261 · DOI
  9. Quinn, 1993, J. Biol. Chem., 268, 20983, 10.1016/S0021-9258(19)36882-6 · DOI
  10. Abo, 1992, J. Biol. Chem., 267, 16767, 10.1016/S0021-9258(18)41846-7 · DOI
  11. Rotrosen, 1992, Science, 256, 1459, 10.1126/science.1318579 · DOI
  12. Heyworth, 1993, Mol. Biol. Cell, 4, 1217, 10.1091/mbc.4.11.1217 · DOI
  13. Diekmann, 1991, Nature, 351, 400, 10.1038/351400a0 · DOI
  14. Settleman, 1992, Nature, 359, 153, 10.1038/359153a0 · DOI
  15. Heisterkamp, 1993, J. Biol. Chem., 268, 16903, 10.1016/S0021-9258(19)85281-X · DOI
  16. Cicchetti, 1992, Science, 257, 803, 10.1126/science.1379745 · DOI
  17. Grand, 1991, Biochem. J., 279, 609, 10.1042/bj2790609 · DOI
  18. Marshall, 1993, Trends Biol. Sci., 18, 250, 10.1016/0968-0004(93)90175-M · DOI
  19. Marshall, 1991, Mol. Cell. Biol., 11, 3997
  20. Sigal, 1986, Proc. Natl. Acad. Sci. U. S. A., 83, 4725, 10.1073/pnas.83.13.4725 · DOI
  21. Zhang, 1990, Science, 249, 162, 10.1126/science.2115210 · DOI
  22. Burstein, 1992, J. Biol. Chem., 267, 22715, 10.1016/S0021-9258(18)50006-5 · DOI
  23. Grussenmeyer, 1985, Proc. Natl. Acad. Sci. U. S. A., 82, 7952, 10.1073/pnas.82.23.7952 · DOI
  24. Summers, 1988, Tex. Agric. Exp. Stn. Bull., 1555
  25. Knaus, 1992, J. Biol. Chem., 267, 23575, 10.1016/S0021-9258(18)35877-0 · DOI
  26. Schaber, 1989, Proteins: Struct. Funct. Genet., 6, 306, 10.1002/prot.340060313 · DOI
  27. Vogel, 1988, Nature, 335, 90, 10.1038/335090a0 · DOI
  28. Krengel, 1990, Cell, 62, 539, 10.1016/0092-8674(90)90018-A · DOI
  29. Gideon, 1992, Mol. Cell. Biol., 12, 2050
  30. Adari, 1988, Science, 240, 518, 10.1126/science.2833817 · DOI
  31. Wittinghofer, 1991, Trends Biochem. Sci., 16, 382, 10.1016/0968-0004(91)90156-P · DOI
  32. Wittinghofer, 1992, Cancer Biol., 3, 189
  33. Self, 1993, Oncogene, 8, 655
  34. Morii, 1993, J. Biol. Chem., 268, 27160, 10.1016/S0021-9258(19)74231-8 · DOI
  35. Wood, 1994, J. Biol. Chem., 269, 5322, 10.1016/S0021-9258(17)37690-1 · DOI
  36. Hu, 1993, Biochemistry, 32, 6763, 10.1021/bi00077a031 · DOI

Cited by

Metadata

Authors
Xu, X., Barry, D. C., Settleman, J., Schwartz, M. A., & Bokoch, G. M.
Venue
Journal of Biological Chemistry
Publisher
Elsevier BV
Published
1994-09
Volume
269
Issue
38
Pages
23569-23574
ISSN
0021-9258
DOI
10.1016/s0021-9258(17)31553-3
Cited by
79
References
0
Language
en
Licence

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review