The DOI matches its Crossref record; 36 of the 36 references it cites were found in a registry or an index.
Mitochondrial uncoupling protein from mouse brown fat. Molecular cloning, genetic mapping, and mRNA expression
Open accessAbstract
We have identified cDNAs clones for several cold-inducible mRNAs from the brown adipose tissue of mice. pCIN-1, a plasmid with a 900-base pair insert, encoded the mitochondrial uncoupling protein (UCP) as determined by the ability of the cDNA insert to select, by hybridization, an mRNA that could be translated into a 32,000-Da protein immunoprecipitable with anti-UCP antibodies. Nine tissues were analyzed; however, UCP cDNA hybridized to an mRNA species of 1.6 and 2.0 kilobase pairs only in brown adipose tissue. A maximum induction of 10-fold occurred within 6 h of exposure to cold (5 degrees C). A BamHI restriction fragment polymorphism detected by Southern blot analysis of genomic DNA in recombinant inbred mouse strains allowed us to map the UCP gene to Chromosome 8. The analysis of the UCP gene expression in diabetic (db) and obese (ob) mice maintained at 27 degrees C for 3 days followed by cold exposure for 4 h at 5 degrees C indicated that UCP mRNA levels in mutant mice were unaffected at 27 degrees C and only slightly reduced at 5 degrees C. Accordingly, the inability of diabetic and obese mice to thermoregulate is not associated with a lack of UCP mRNA induction.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The source backs part of the claim, not all of it — or backs it for a different population, condition or comparison.
In the citing paper
“Importantly, although strong evidence of the mitochondrial uncoupling property is clear for UCP-1 [7,12], it is still heavily debated for the other UCPs [13,14,15].”
From Mitochondrial Uncoupling: A Key Controller of Biological Processes in Physiology and Diseases (2019) · cites this paper as [7]
Experimental — accuracy not yet measuredIn the citing paper
“UCP-1 is a transmembrane protein localized in the inner mitochondrial membrane catalyzing the transport of protons across the mitochondrial membrane and thereby inducing mitochondrial uncoupling [7].”
From Mitochondrial Uncoupling: A Key Controller of Biological Processes in Physiology and Diseases (2019) · cites this paper as [7]
In the citing paper
“In this tissue, electron transport is greatly accelerated due to tissue-specific expression of the mitochondrial UCP1.”
From The role of mitochondria in the pathogenesis of type 2 diabetes (2010) · cites this paper as [154]
In the citing paper
“UCP1 hinders the establishment of, or dissipates, a proton gradient of sufficient magnitude to sustain the synthetic activity of the mitochondrial ATPase (150, 153-155), thus driving continuous accelerated electron transport.”
From The role of mitochondria in the pathogenesis of type 2 diabetes (2010) · cites this paper as [154]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
LAEMMLI, U. K. (1970) · Nature · doi:10.1038/227680a0 · Cited by 249,961
Rigby, P. W. J., Dieckmann, M., Rhodes, C., & Berg, P. R. (1977) · Journal of Molecular Biology · doi:10.1016/0022-2836(77)90052-3 · Cited by 11,400
Quantitative Film Detection of 3H and 14C in Polyacrylamide Gels by Fluorography
Laskey, R. A., & Mills, A. D. (1975) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1975.tb02238.x · Cited by 4,629
Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.
Grunstein, M. R., & Hogness, D. S. (1975) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.72.10.3961 · Cited by 3,386
Thermogenic mechanisms in brown fat.
Nicholls, D. G., & Locke, R. M. (1984) · Physiological Reviews · doi:10.1152/physrev.1984.64.1.1 · Cited by 1,602
Genetic variants and strains of the laboratory mouse
(1990) · Choice Reviews Online · doi:10.5860/choice.28-1324 · Cited by 1,492
NON‐SHIVERING THERMOGENESIS AND ITS THERMOREGULATORY SIGNIFICANCE
Janský, L. (1973) · Biological reviews/Biological reviews of the Cambridge Philosophical Society · doi:10.1111/j.1469-185x.1973.tb01115.x · Cited by 612
Transcriptional control in the production of liver-specific mRNAs
Derman, E., Krauter, K. S., Walling, L. L., Weinberger, C., Ray, M., & Darnell, J. E., Jr. (1981) · Cell · doi:10.1016/0092-8674(81)90436-0 · Cited by 550
Foster, D. O., & Frydman, M. L. (1979) · Canadian Journal of Physiology and Pharmacology · doi:10.1139/y79-039 · Cited by 518
Maccecchini, M. L., Rudin, Y., Blobel, G., & Schatz, G. (1979) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.76.1.343 · Cited by 453
Heaton, G. M., Wagenvoord, R. J., Kemp, A., & Nicholls, D. G. (1978) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1978.tb12045.x · Cited by 407
Biogenic amines in development
Curzon, G. (1981) · Early Human Development · doi:10.1016/0378-3782(81)90059-1 · Cited by 323
Parnes, J. R., Velan, B., Felsenfeld, A. L., Ramanathan, L., Ferrini, U., Appella, E., & Seidman, J. G. (1981) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.78.4.2253 · Cited by 314
Dobberstein, B., Garoff, H., Warren, G., & Robinson, P. J. (1979) · Cell · doi:10.1016/0092-8674(79)90316-7 · Cited by 271
Ricquier, D., & Kader, J. (1976) · Biochemical and Biophysical Research Communications · doi:10.1016/0006-291x(76)90849-4 · Cited by 269
Thermoregulation and non-shivering thermogenesis in the genetically obese (ob/ob) mouse
Trayhurn, P., & James, W. P. T. (1978) · Pflügers Archiv - European Journal of Physiology · doi:10.1007/bf00584859 · Cited by 254
Bouillaud, F., Ricquier, D., Thibault, J., & Weissenbach, J. (1985) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.82.2.445 · Cited by 244
Cheley, S., & Anderson, R. (1984) · Analytical Biochemistry · doi:10.1016/0003-2697(84)90339-7 · Cited by 243
Nedergaard, J., & Lindberg, O. (1982) · International review of cytology · doi:10.1016/s0074-7696(08)61173-0 · Cited by 232
Himms‐Hagen, J., & Desautels, M. (1978) · Biochemical and Biophysical Research Communications · doi:10.1016/0006-291x(78)91036-7 · Cited by 210
Desautels, M., Zaror‐Behrens, G., & Himms‐Hagen, J. (1978) · Canadian Journal of Biochemistry · doi:10.1139/o78-060 · Cited by 179
Imperfect Homeothermia in the Hereditary Obese-Hyperglycemic Syndrome of Mice
Davis, T. R. A., & Mayer, J. (1954) · American Journal of Physiology-Legacy Content · doi:10.1152/ajplegacy.1954.177.2.222 · Cited by 175
Zimmermann, R., & Neupert, W. (1980) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1980.tb04787.x · Cited by 167
Exclusive occurrence of thermogenin antigen in brown adipose tissue
Cannon, B., Hedin, A., & Nedergaard, J. (1982) · FEBS Letters · doi:10.1016/0014-5793(82)81319-7 · Cited by 164
Shore, G. C., Carignan, P., & Raymond, Y. (1979) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)50731-6 · Cited by 114
Lean, M. E. J., Branch, W. J., James, W. P. T., Jennings, G., & Ashwell, M. (1983) · Bioscience Reports · doi:10.1007/bf01121572 · Cited by 85
Taub, F. E., & Thompson, E. B. (1982) · Analytical Biochemistry · doi:10.1016/0003-2697(82)90133-6 · Cited by 77
Ashwell, M., Jennings, G., Richard, D., Stirling, D. M., & Trayhurn, P. (1983) · FEBS Letters · doi:10.1016/0014-5793(83)80740-6 · Cited by 68
Supowit, S. C., Potter, E., Evans, R. M., & Rosenfeld, M. G. (1984) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.81.10.2975 · Cited by 67
Mihara, K., Omura, T., Harano, T., Brenner, S. C., Fleischer, S., Rajagopalan, K. V., & Blobel, G. (1982) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)34779-3 · Cited by 59
Ricquier, D., Thibault, J., Bouillaud, F., & Kuster, Y. (1983) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)32265-8 · Cited by 56
Kozak, L. P., & Birkenmeier, E. H. (1983) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.80.10.3020 · Cited by 46
Self-citationAshwell, M., Holt, S., Jennings, G., Stirling, D. M., Trayhurn, P., & York, D. A. (1985) · FEBS Letters · doi:10.1016/0014-5793(85)80525-1 · Cited by 46
Synthesis in vitro of rat brown adipose tissue 32000 Mr protein
Freeman, K. B., Chien, S.-M., Litchfield, D. W., & Patel, H. V. (1983) · FEBS Letters · doi:10.1016/0014-5793(83)80606-1 · Cited by 31
Kozak, L. P. (1985) · Genetics · doi:10.1093/genetics/110.1.123 · Cited by 17
Self-citationKozak, L. P., & Burkart, D. L. (1981) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)69380-4 · Cited by 12
Self-citation
Research works printed but not linked: 6
- Krawetz, 1984, Biotechniques, 2, 342
- Moutier, 1978, Mouse Newsletter, 58, 64
- Sundin, 1980, Comp. Biochem. Biophys., 65B, 463
- Nedergaard, 1985, Am. J. Physiol.
- Hogan, 1980, Am. J. Physiol., 239, E301
- Zaror-Behrens, 1983, Am. J. Physiol., 244, E361
Cited by
Sorted by citations
Brown Adipose Tissue: Function and Physiological Significance
CANNON, B., & NEDERGAARD, J. (2004) · Physiological Reviews · doi:10.1152/physrev.00015.2003 · Cited by 6,433
Wu, Z., Puigserver, P., Andersson, U., Zhang, C., Adelmant, G., Mootha, V., Troy, A., Cinti, S., Lowell, B., Scarpulla, R. C., et al. (1999) · Cell · doi:10.1016/s0092-8674(00)80611-x · Cited by 4,139
A Cold-Inducible Coactivator of Nuclear Receptors Linked to Adaptive Thermogenesis
Puigserver, P., Wu, Z., Park, C. W., Graves, R., Wright, M., & Spiegelman, B. M. (1998) · Cell · doi:10.1016/s0092-8674(00)81410-5 · Cited by 3,864
Defects in Adaptive Energy Metabolism with CNS-Linked Hyperactivity in PGC-1α Null Mice
Lin, J., Wu, P.-H., Tarr, P. T., Lindenberg, K. S., St-Pierre, J., Zhang, C.-Y., Mootha, V. K., Jäger, S., Vianna, C. R., Reznick, R. M., et al. (2004) · Cell · doi:10.1016/j.cell.2004.09.013 · Cited by 1,162
Development of obesity in transgenic mice after genetic ablation of brown adipose tissue
Lowell, B. B., S-Susulic, V., Hamann, A., Lawitts, J. A., Himms-Hagen, J., Boyer, B. B., Kozak, L. P., & Flier, J. S. (1993) · Nature · doi:10.1038/366740a0 · Cited by 1,075
Self-citation
Metadata
- Authors
- Jacobsson, A., Stadler, U., Glotzer, M. A., & Kozak, L. P.
- Affiliations
- Stockholm University (SE)
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1985-12
- Volume
- 260
- Issue
- 30
- Pages
- 16250-16254
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1513078811
- Cited by
- 300
- References
- 36
- Citations by year
- 2026: 2 · 2025: 3 · 2024: 2 · 2023: 2 · 2022: 6 · 2021: 10 · 2020: 1 · 2019: 4 · 2018: 8 · 2017: 7 · 2016: 7 · 2015: 1 · 2014: 2 · 2013: 3 · 2012: 3
- FWCI
- 6.62
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Biological Chemistry (publishedVersion, cc-by)
- PubMed (publishedVersion)
- The Mouseion at the JAXlibrary (Jackson Laboratory) (submittedVersion)
- Funders
- National Institutes of Health
- Eunice Kennedy Shriver National Institute of Child Health and Human Development (HD-08431)
- Topics
- Health Sciences › Medicine › Physiology (medical) › Adipose Tissue and Metabolism
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Peroxisome Proliferator-Activated Receptors
- Keywords
- mitochondrial uncoupling proteins, brown adipose tissue, cold-responsive genes, cDNA cloning, gene mapping, chromosome 8, mRNA expression, cold exposure, thermoregulation, diabetic mice, recombinant inbred mouse strains, restriction fragment polymorphism
- MeSH terms
- Uncoupling Protein 1, Animals, Adipose Tissue, Brown, Carrier Proteins, Cloning, Molecular, Cold Temperature, DNA Transposable Elements, DNA, Mitochondrial, Genes, Ion Channels, Membrane Proteins, Mice, Inbred C57BL, Mice, Mutant Strains, Mitochondria, Molecular Weight, RNA, Messenger, Protein Biosynthesis, Mitochondrial Proteins, Mice
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
