The DOI matches its Crossref record; 28 of the 28 references it cites were found in a registry or an index.
Androgens increase intracellular calcium concentration and inositol 1,4,5-trisphosphate and diacylglycerol formation via a pertussis toxin-sensitive G-protein.
Open accessAbstract
Bone is a target tissue of androgens, but the mechanisms by which they act on bone are still unclear. This study examines the early (5-60 s) effects of 1 pM to 1 microM testosterone on cytosolic free Ca2+ concentration ([Ca2+]i) and inositol 1,4,5-trisphosphate (InsP3) and diacyglycerol (DAG) formation in confluent male rat osteoblasts. 10 pM to 10 nM testosterone increased [Ca2+]i within 5 s via Ca2+ influx as shown by the effects of EGTA and the Ca2+ channel blockers nifedipine and verapamil and via Ca2+ mobilization from the endoplasmic reticulum as shown by the effects of thapsigargin and neomycin. 10 pM to 10 nM testosterone increased InsP3 and DAG formation within 10 s. Testosterone immobilized on bovine serum albumin (testosterone (O-carboxymethyl)oxime/bovine serum albumin) and its derivative, (O-carboxymethyl)oxime, rapidly increased [Ca2+]i and InsP3 and DAG formation and were full agonists, although they were less potent than the free steroid. Cyproterone acetate, a nuclear antagonist, did not block the increase in [Ca2+]i and InsP3 and DAG formation induced by testosterone. Finally, neomycin and pertussis toxin totally abolished the effects of testosterone on InsP3 and DAG. These results suggest that male rat osteoblasts bear nongenomic unconventional cell-surface receptors for testosterone that belong to the class of the membrane receptors coupled to a phospholipase C via a pertussis toxin-sensitive G-protein.
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Cited by
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The Roles of Androgen Receptors and Androgen-Binding Proteins in Nongenomic Androgen Actions
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Steroid-hormone rapid actions, membrane receptors and a conformational ensemble model
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Non-genomic actions of androgens
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Metadata
- Authors
- Lieberherr, M., & Grosse, B.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1994-03
- Volume
- 269
- Issue
- 10
- Pages
- 7217-7223
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1576763630
- Cited by
- 275
- References
- 28
- FWCI
- 4.70
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Lieberherr, M., & Grosse, B. (1994). Androgens increase intracellular calcium concentration and inositol 1,4,5-trisphosphate and diacylglycerol formation via a pertussis toxin-sensitive G-protein. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(17)37270-8
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