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Cited by 202

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Mitochondrial DNA in mortal and immortal human cells. Genome number, integrity, and methylation.

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Shmookler Reis, R J, Goldstein, S·1983·Journal of Biological Chemistry
DOI 10.1016/s0021-9258(17)44633-3
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Abstract

Mitochondrial DNA was quantitated in total DNA of various normal and mutant strains of human diploid fibroblasts (finite replicative lifespan) and permanent cell lines, using Southern-transfer hybridization to 32P-labeled pure mtDNA probe and saturation hybridization to 3H-labeled cRNA copied from mtDNA. In six normal fibroblast strains, mtDNA copy number increased during serial passage roughly in proportion to cell volume or protein content, whereas normalized mtDNA content per pg of protein depended upon in vivo donor age but not passage level ("in vitro" age). Copy numbers for mtDNA varied much more widely in individual fibroblast clones than in mass cultures, but were not well correlated with longevity or growth rate. Five mutant fibroblast strains associated with reduced replicative lifespan, and four permanent cell lines, were also examined; in each group, mtDNA values were observed both lower and higher than any obtained for normal fibroblasts. No evidence was found of petite-type deletions from human mtDNA, either at late passage or in individual clones of fibroblasts. Methylation of mtDNA genomes was strikingly non-random and apparently decreased with culture age.

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Metadata

Authors
Shmookler Reis, R. J., & Goldstein, S.
Venue
Journal of Biological Chemistry
Publisher
Elsevier BV
Published
1983-08
Volume
258
Issue
15
Pages
9078-9085
ISSN
0021-9258 (ISSN-L)
DOI
10.1016/s0021-9258(17)44633-3
OpenAlex
W1519160353
Cited by
202
References
1
FWCI
1.99
Type
journal article
Language
en
Open access
hybrid
Licence

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CoChat. (2026). Citation check: Mitochondrial DNA in mortal and immortal human cells. Genome number, integrity, and methylation. https://app.cochat.ai/papers/10.1016/s0021-9258(17)44633-3
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Shmookler Reis, R. J., & Goldstein, S. (1983). Mitochondrial DNA in mortal and immortal human cells. Genome number, integrity, and methylation. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(17)44633-3

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