Cited by 396Immune Response and InflammationProtein Kinase Regulation and GTPase SignalingNF-κB Signaling Pathways

Bacterial lipopolysaccharide induces tyrosine phosphorylation and activation of mitogen-activated protein kinases in macrophages.

Open access
Weinstein, S.L., Sanghera, J.S., Lemke, K, DeFranco, A.L., Pelech, S.L.·1992·Journal of Biological Chemistry

Abstract

Bacterial lipopolysaccharide (LPS) is a potent activator of antibacterial responses by macrophages. Following LPS stimulation, the tyrosine phosphorylation of several proteins is rapidly increased in macrophages, and this event appears to mediate some responses to LPS. We now report that two of these tyrosine phosphoproteins of 41 and 44 kDa are isoforms of mitogen-activated protein (MAP) kinase. Each of these proteins was reactive with anti-MAP kinase antibodies and comigrated with MAP kinase activity in fractions eluted from a MonoQ anion-exchange column. Following LPS stimulation, column fractions containing the tyrosine phosphorylated forms of p41 and p44 exhibited increased MAP kinase activity. Inhibition of LPS-induced tyrosine phosphorylation of these proteins was accompanied by inhibition of MAP kinase activity. Additionally, induction of p41/p44 tyrosine phosphorylation and MAP kinase activity by LPS appeared to be independent of activation of protein kinase C, even though phorbol esters also induced these responses. These results demonstrate that LPS induces the tyrosine phosphorylation and activation of at least two MAP kinase isozymes. Since MAP kinases appear to modulate cellular processes in response to extracellular signals, these kinases may be important targets for LPS action in macrophages.

Integrity signals

Registry

Crossref · 10.1016/s0021-9258(18)42133-3

Cites

31 of 31 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

We couldn’t retrieve this paper’s full text, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • LPS activates three subclasses of MAPKs in macrophages and other cells: ERK, JNK, and p38; some compounds suppress proinflammatory cytokines and NF-κB and inhibit COX-2 and iNOS expression by inhibiting some of these MAPKs.

    “In macrophages and other types of cells, LPS activates three subclasses of MAPKs: extracellular signal-regulated kinase (ERK), c-Jun-terminal kinase (JNK) and p38. Studies have revealed that some compounds are able to suppress proinflammatory cytokines and NF-κB, to inhibit the COX-2 and iNOS expression inhibiting some of these MAPKS [129,130,131].”

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Weinstein, S. L., Sanghera, J. S., Lemke, K., DeFranco, A. L., & Pelech, S. L.
Affiliations
  • University of California, San Francisco (US)
  • Genentech (US)
Venue
Journal of Biological Chemistry
Publisher
Elsevier BV
Published
1992-07
Volume
267
Issue
21
Pages
14955-14962
ISSN
0021-9258 (ISSN-L)
OpenAlex
W1671728707
Cited by
396
References
31
Citations by year
2026: 2 · 2024: 3 · 2022: 2 · 2021: 2 · 2020: 1 · 2019: 4 · 2018: 5 · 2017: 8 · 2016: 4 · 2015: 4 · 2014: 4 · 2013: 4 · 2012: 8
FWCI
8.52
Type
journal article
Language
en
Open access
Locations
  • Journal of Biological Chemistry (publishedVersion, cc-by)
  • PubMed (publishedVersion)
Funders
National Institute of Allergy and Infectious Diseases (AI20038)
Topics
  • Life Sciences › Immunology and Microbiology › Immunology › Immune Response and Inflammation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › NF-κB Signaling Pathways
Keywords
lipopolysaccharide, macrophages, tyrosine phosphorylation, mitogen-activated protein kinase, signal transduction, phorbol ester, antibacterial response
MeSH terms
Amino Acid Sequence, Animals, Cells, Cultured, Chromatography, Ion Exchange, Electrophoresis, Polyacrylamide Gel, Enzyme Activation, Isoenzymes, Lipopolysaccharides, Macrophages, Molecular Sequence Data, Peptides, Phosphorylation, Precipitin Tests, Protein Kinase C, Protein Kinases, Quinones, Tyrosine, Blotting, Western, Benzoquinones, Rifabutin, Calcium-Calmodulin-Dependent Protein Kinases, Lactams, Macrocyclic, Protein Kinase Inhibitors, Mice

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review