The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
Molecular cloning and expression of a cDNA encoding endothelial cell nitric oxide synthase.
Open accessAbstract
Endothelium-derived relaxing factor (EDRF), identified as nitric oxide (NO), is derived from a guanidino nitrogen of L-arginine via its metabolism by nitric oxide synthase (NOS). Herein, we report the molecular cloning of a cDNA encoding the constitutive calcium-calmodulin (Ca2+/CaM)-regulated nitric oxide synthase (ECNOS). A full-length ECNOS clone was isolated by screening a bovine aortic endothelial cell cDNA library using a fragment of rat brain NOS (bNOS) cDNA. This cDNA has an open reading frame of 3615 nucleotides encoding a 1205-amino acid protein. Membranes prepared from COS cells transfected with the ECNOS cDNA demonstrated NADPH- and Ca2+/CaM- dependent conversion of L-, but not D-, arginine to NO and citrulline that was inhibited by NG-nitro-L-arginine methyl ester. Comparison of the deduced amino acid sequence of ECNOS to the bNOS and macrophage NOS (Mac-NOS) sequences revealed 57 and 50% identity, respectively. In addition, ECNOS contains a unique N-myristylation consensus sequence (not shared by bNOS or Mac-NOS) that may explain its membrane localization.
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Nitric oxide: physiology, pathophysiology, and pharmacology.
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Nitric oxide synthases in mammals
Knowles, R. G., & Moncada, S. (1994) · Biochemical Journal · doi:10.1042/bj2980249 · Cited by 2,752
Laminar Shear Stress: Mechanisms by Which Endothelial Cells Transduce an Atheroprotective Force
Traub, O., & Berk, B. C. (1998) · Arteriosclerosis, Thrombosis, and Vascular Biology · doi:10.1161/01.atv.18.5.677 · Cited by 1,085
Gases as biological messengers: nitric oxide and carbon monoxide in the brain
Dawson, T., & Snyder, S. (1994) · The Journal of Neuroscience · doi:10.1523/jneurosci.14-09-05147.1994 · Cited by 1,043
Hambrecht, R., Fiehn, E., Weigl, C., Gielen, S., Hamann, C., Kaiser, R., Yu, J., Adams, V., Niebauer, J., & Schuler, G. (1998) · Circulation · doi:10.1161/01.cir.98.24.2709 · Cited by 963
Metadata
- Authors
- Sessa, W. C., Harrison, J. K., Barber, C. M., Zeng, D., Durieux, M. E., D'Angelo, D. D., Lynch, K. R., & Peach, M. J.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1992-08
- Volume
- 267
- Issue
- 22
- Pages
- 15274-15276
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1511098597
- Cited by
- 467
- References
- 3
- FWCI
- 26.40
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Sessa, W. C., Harrison, J. K., Barber, C. M., Zeng, D., Durieux, M. E., D'Angelo, D. D., Lynch, K. R., & Peach, M. J. (1992). Molecular cloning and expression of a cDNA encoding endothelial cell nitric oxide synthase. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(19)49528-8
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