The DOI matches its Crossref record; 19 of the 19 references it cites were found in a registry or an index.
Solubilization, purification, and characterization of an inositol trisphosphate receptor.
Open accessAbstract
Inositol 1,4,5-trisphosphate is a second messenger of the phosphoinositide system which can mobilize calcium from intracellular stores. Rat cerebellum is an abundant source of a receptor for inositol 1,4,5-trisphosphate (Worley, P. F., Baraban, J. M., Supattapone, S., Wilson, V. S., and Snyder, S. H. (1987) J. Biol. Chem. 262, 12132-12136). In this study we have solubilized and purified this receptor to apparent homogeneity from rat cerebellum. Crude membrane, detergent-solubilized, and purified receptor preparations display similar selectivity for inositol 1,4,5-trisphosphate over other inositol phosphates. The purified receptor is globular with a Stokes' radius of approximately 10 nm. Electrophoretic analysis reveals one protein band with an Mr of 260,000. While binding is reversibly inhibited by 300 nM calcium in particulate fractions and detergent-solubilized membranes, the purified protein is not inhibited by calcium concentrations up to 1.5 mM. Inhibition by calcium is reconstituted by addition of detergent-solubilized cerebellar membranes, but not by the cytosolic fraction of cerebellum.
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Characterization of inositol trisphosphate receptor binding in brain. Regulation by pH and calcium.
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Primary structure and functional expression of the inositol 1,4,5-trisphosphate-binding protein P400
Furuichi, T., Yoshikawa, S., Miyawaki, A., Wada, K., Maeda, N., & Mikoshiba, K. (1989) · Nature · doi:10.1038/342032a0 · Cited by 1,049
Conformational motions and ligand-binding underlying gating and regulation in IP3R channel
Fan, G., Baker, M. R., Terry, L. E., Arige, V., Chen, M., Seryshev, A. B., Baker, M. L., Ludtke, S. J., Yule, D. I., & Serysheva, I. I. (2022) · Nature Communications · doi:10.1038/s41467-022-34574-1 · Cited by 52
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Metadata
- Authors
- Supattapone, S., Worley, P. F., Baraban, J. M., & Snyder, S. H.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1988-01
- Volume
- 263
- Issue
- 3
- Pages
- 1530-1534
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1598081743
- Cited by
- 649
- References
- 19
- FWCI
- 34.12
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Supattapone, S., Worley, P. F., Baraban, J. M., & Snyder, S. H. (1988). Solubilization, purification, and characterization of an inositol trisphosphate receptor. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(19)57336-7
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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