CoChat
Sign Up
Cited by 153

The DOI matches its Crossref record, with a small difference in title or year; 1 of the 1 references it cites were found in a registry or an index.

Studies of the Regulatory Mechanism of Ca2+/Calmodulin-dependent protein kinase II

Fong, Y L, Taylor, W L, Means, A R, Soderling, T R·1989·Journal of Biological Chemistry
DOI 10.1016/s0021-9258(19)84770-1
Open this paper in CoChat — free account

Abstract

A cDNA clone for the alpha subunit of mouse brain Ca2+/CaM-dependent protein kinase II (CaM-kinase II) was transcribed in vitro and translated in a rabbit reticulocyte lysate system. Inclusion of [35S]methionine in the translation system yielded a single 35S-polypeptide of about 50 kDa. When the translation system was assayed for CaM-kinase II activity, there was a 5-10-fold enrichment of kinase activity which was totally dependent on Ca2+/calmodulin (CaM). Both the 50-kDa 35S-polypeptide and the Ca2+/CaM-dependent protein kinase activity were quantitatively immunoprecipitated by rat brain CaM-kinase II antibody. When the translated wild-type kinase was subjected to autophosphorylation conditions in the presence of Ca2+, CaM, Mg2+, and ATP, the Ca2+-independent activity (assayed in the presence of [ethylenebis(oxyethylenenitrilo)]tetraacetic acid) increased from 5.8 +/- 0.7 to 26.5 +/- 2.1% of total activity (assayed in the presence of Ca2+/CaM). These properties confirm the identity of the kinase translated in vitro as CaM-kinase II. The role of Thr-286 autophosphorylation in formation of the Ca2+-independent activity was investigated by site-directed mutation of Thr-286 to Ala (Ala-286 kinase) and to Asp (Asp-286 kinase). The Ala-286 kinase was completely dependent on Ca2+/CaM for activity prior and subsequent to autophosphorylation. The Asp-286 kinase exhibited 21.9 +/- 0.8% Ca2+-independent activity, and this was not increased by autophosphorylation. These results establi…

Overview

Integrity signals

Registry

Crossref · 10.1016/s0021-9258(19)84770-1

Cites

1 of 1 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Citations

Cites

Flagged first, then by citations

Research works printed but not linked: 29

  1. Nairn, 1985, Annu. Rev. Biochem., 54, 931, 10.1146/annurev.bi.54.070185.004435 · DOI
  2. Kennedy, 1983, Annu. Reu. Neurosci., 6, 493, 10.1146/annurev.ne.06.030183.002425 · DOI
  3. Colbran, 1989, Biochem. J., 258, 313, 10.1042/bj2580313 · DOI
  4. Schulman, 1989, Trends Biochem. Sci., 14, 62, 10.1016/0968-0004(89)90045-5 · DOI
  5. Hanley, 1987, Science, 237, 293, 10.1126/science.3037704 · DOI
  6. Lin, 1987, Proc. Natl. Acad. Sci. U. S. A., 84, 5962, 10.1073/pnas.84.16.5962 · DOI
  7. Bulleit, 1988, Neuron, 1, 63, 10.1016/0896-6273(88)90210-3 · DOI
  8. Bennett, 1987, Proc. Natl. Acad. Sci. U. S. A., 84, 1794, 10.1073/pnas.84.7.1794 · DOI
  9. Schworer, 1988, J. Biol. Chem., 263, 13486, 10.1016/S0021-9258(18)68264-X · DOI
  10. Thiel, 1988, Proc. Natl. Acad. Sci. U. S. A., 85, 6337, 10.1073/pnas.85.17.6337 · DOI
  11. Miller, 1988, Neuron, 1, 593, 10.1016/0896-6273(88)90109-2 · DOI · Paper page
  12. Schworer, 1986, J. Biol. Chem., 261, 8581, 10.1016/S0021-9258(19)84416-2 · DOI · Paper page
  13. Hashimoto, 1987, J. Biol. Chem., 262, 8051, 10.1016/S0021-9258(18)47525-4 · DOI · Paper page
  14. Miller, 1986, Cell, 44, 861, 10.1016/0092-8674(86)90008-5 · DOI · Paper page
  15. Colbran, 1988, J. Biol. Chem., 263, 18145, 10.1016/S0021-9258(19)81337-6 · DOI
  16. Payne, 1988, J. Biol. Chem., 263, 7190, 10.1016/S0021-9258(18)68626-0 · DOI
  17. Colbran, 1989, J. Biol. Chem., 264, 4800, 10.1016/S0021-9258(18)83661-4 · DOI · Paper page
  18. Lickteig, 1988, J. Biol. Chem., 263, 19233, 10.1016/S0021-9258(18)37414-3 · DOI
  19. Gopalakrishna, 1982, Biochem. Biophys. Res. Commun., 104, 830, 10.1016/0006-291X(82)90712-4 · DOI
  20. Ohta, 1988, J. Biol. Chem., 263, 11540, 10.1016/S0021-9258(18)37992-4 · DOI
  21. Hashimoto, 1987, Arch. Biochem. Biophys., 252, 418, 10.1016/0003-9861(87)90048-8 · DOI
  22. Waxham, 1989, J. Biol. Chem., 264, 7477, 10.1016/S0021-9258(18)83259-8 · DOI
  23. Sanger, 1977, Proc. Natl. Acad. Sci. U. S. A., 74, 5463, 10.1073/pnas.74.12.5463 · DOI · Paper page
  24. Krieg, 1987, Methods Enzymol., 155, 397, 10.1016/0076-6879(87)55027-3 · DOI
  25. Pongor, 1987, Methods Enzymol., 154, 450, 10.1016/0076-6879(87)54090-3 · DOI
  26. Dayhoff, 1983, Methods Enzymol., 91, 524, 10.1016/S0076-6879(83)91049-2 · DOI
  27. Gamier, 1978, J. Mol. Biol., 120, 97, 10.1016/0022-2836(78)90297-8 · DOI
  28. Herrera, 1988, J. Biol. Chem., 263, 5560, 10.1016/S0021-9258(18)60601-5 · DOI
  29. Kwiatkowski, 1988, J. Biol. Chem., 263, 6484, 10.1016/S0021-9258(18)68667-3 · DOI

Cited by

Sorted by citations

Metadata

Authors
Fong, Y. L., Taylor, W. L., Means, A. R., & Soderling, T. R.
Venue
Journal of Biological Chemistry
Publisher
Elsevier BV
Published
1989-10
Volume
264
Issue
28
Pages
16759-16763
ISSN
0021-9258
DOI
10.1016/s0021-9258(19)84770-1
Cited by
153
References
1
Language
en
Licence

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review