Quantification of apolipoproteins B-100, B-48, and E in human triglyceride-rich lipoproteins
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We have developed a procedure to quantify apolipoprotein (apo) B-100, apoB-48, and apoE in human triglyceride-rich lipoproteins. This procedure permits delipidation of small amounts of triglyceride-rich lipoproteins without appreciable losses, and quantification of these apolipoproteins in samples containing as little as 10 micrograms of protein. Delipidated triglyceride-rich lipoproteins are subjected to sodium dodecyl sulfate polyacrylamide slab gel electrophoresis, and the mass of apolipoproteins is estimated after densitometric scanning and volume integration of Coomassie blue-stained bands. The chromogenicities of apoB-100 and apoB-48 are virtually identical, and twofold lower than that of apoE. The standard curve for each apolipoprotein follows a power function over a wide protein range, permitting quantification of as little as 0.2 microgram of apoB-48 and as much as 30 micrograms of apoB-100 from a single application of triglyceride-rich lipoproteins to the gels. This method is suitable for routine use in studies of the intestinal and hepatic contributions to triglyceride-rich lipoproteins and their responses to postprandial lipemia.
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The chromogenicities of human apoB-48 and apoB-100 are equal.
“The chromogenicities of human apoB-48 and apoB-100 have been shown to be equal (24).”
From Omega-3 fatty acid supplementation accelerates chylomicron triglyceride clearance (2003) · cites this paper as [24]
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- Authors
- Kotite, L., Bergeron, N., & Havel, R. J.
- Affiliations
- University of California, San Francisco (US)
- Venue
- Journal of Lipid Research
- Publisher
- Elsevier BV
- Published
- 1995-04
- Volume
- 36
- Issue
- 4
- Pages
- 890-900
- ISSN
- 0022-2275 (ISSN-L)
- OpenAlex
- W2412088269
- Cited by
- 75
- References
- 28
- Citations by year
- 2018: 2 · 2016: 1 · 2014: 1 · 2013: 3
- FWCI
- 6.10
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Lipid Research (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- National Institutes of Health (5p50hl014237-25)
- National Heart, Lung, and Blood Institute (HL 14237)
- Topics
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Lipid metabolism and disorders
- Health Sciences › Medicine › Surgery › Lipoproteins and Cardiovascular Health
- Health Sciences › Medicine › Surgery › Cholesterol and Lipid Metabolism
- Keywords
- apolipoprotein B-100, apolipoprotein B-48, apolipoprotein E, triglyceride-rich lipoproteins, delipidation, densitometry, postprandial lipemia, hepatic lipoprotein synthesis
- MeSH terms
- Apolipoproteins B, Apolipoproteins E, Electrophoresis, Polyacrylamide Gel, Humans, Lipoproteins, Triglycerides, Apolipoprotein B-48, Apolipoprotein B-100
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