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Cited by 121

The DOI matches its Crossref record; 20 of the 20 references it cites were found in a registry or an index.

Immortalization of Human Adult Normal Prostatic Epithelial Cells by Liposomes Containing Large T-SV40 Gene

Cussenot, O., Berthon, Ph., Berger, R., Mowszowicz, I., Faille, A., Hojman, F., Teillac, P., Le Duc, A., Calvo, F.·1991·Journal of Urology
DOI 10.1016/s0022-5347(17)37953-3
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Abstract

Simian virus SV40 has been widely used to immortalize epithelial cells of mammalian origin. We report here, for the first time to our knowledge, the immortalization of normal adult prostatic epithelial cells in culture by transfection of a plasmid containing SV40 genome with a defective replication origin (SV40 ori-) encapsulated into liposomes. These cells (PNT1) have now been cultured for more than 12 months, and shown to contain the SV40 genome. They express large T protein, present the phenotype of differentiated luminal prostatic cells (positive with antibodies to cytokeratin 18, 19, weakly positive for prostatic acid phosphatase and prostatic specific antigen, negative with anticytokeratin 14 and KL2 antibody). PNT1 cells contain high affinity receptors for dihydrotestosterone. These cells provide a useful tool to study the biology and the pathology of adult prostatic epithelial cells, specially to understand the steps leading to prostatic transformation.

Overview

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Registry

Crossref · 10.1016/s0022-5347(17)37953-3

Cites

20 of 20 references verified0 citation errors · 0 not checkable

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Citations

Cites

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Research works printed but not linked: 3

  1. Cussenot, 1989, Culture de cellules normales et tumorales de prostate humaine, Annales d’Urologie, 23, 533
  2. I.S.C.N., an international system for human cytogenetic nomenclature. Edited by Harnden and Klinger, published in collaboration with Cytogenetic Cell Genet. Karger, Basel, 1985.
  3. Kraighn, 1979, Establishment characterization of a human prostatic carcinoma cell line (PC3), Invest. Urol., 17, 16

Cited by

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Metadata

Authors
Cussenot, O., Berthon, P., Berger, R., Mowszowicz, I., Faille, A., Hojman, F., Teillac, P., Le Duc, A., & Calvo, F.
Affiliations
From the Department of Urology, Department of Pharmacology, Institute of Molecular Genetics, INSERM Unit 301, Hopital Saint-Louis, and the Department of Biochemistry B, Hopital Necker, Paris, France
Venue
Journal of Urology
Publisher
Ovid Technologies (Wolters Kluwer Health)
Published
1991-09
Volume
146
Issue
3
Pages
881-886
ISSN
  • 0022-5347
  • 1527-3792
DOI
10.1016/s0022-5347(17)37953-3
Cited by
121
References
20
Language
en
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)

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CoChat. (2026). Citation check: Immortalization of Human Adult Normal Prostatic Epithelial Cells by Liposomes Containing Large T-SV40 Gene. https://app.cochat.ai/papers/10.1016/s0022-5347(17)37953-3
The paper
Cussenot, O., Berthon, P., Berger, R., Mowszowicz, I., Faille, A., Hojman, F., Teillac, P., Le Duc, A., & Calvo, F. (1991). Immortalization of Human Adult Normal Prostatic Epithelial Cells by Liposomes Containing Large T-SV40 Gene. Journal of Urology. https://doi.org/10.1016/s0022-5347(17)37953-3

Data sources

Bibliographic data: Semantic Scholar, OpenAlex, Crossref.

Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.