Biosynthesis of 20-hydroxyecdysone in Ajuga hairy roots: The possibility of 7-ene introduction at a late stage

Recent advances in our knowledge of ecdysteroid biosynthesis in insects and crustaceans

Grieneisen, M. L. (1994) · Insect Biochemistry and Molecular Biology · doi:10.1016/0965-1748(94)90078-7 · Cited by 184 · see citing works

Biosynthesis and distribution of insect-molting hormones in plants—A review

Adler, J. H., & Grebenok, R. J. (1995) · Lipids · doi:10.1007/bf02537830 · Cited by 79 · see citing works

Conversion of 24-methylcholesterol to 6-oxo-24-methylcholestanol, a putative intermediate of the biosynthesis of brassinosteroids, in cultured cells of Catharanthus roseus

Suzuki, H., Inoue, T., Fujioka, S., Saito, T., Takatsuto, S., Yokota, T., Murofushi, N., Yanagisawa, T., & Sakurai, A. (1995) · Phytochemistry · doi:10.1016/0031-9422(95)00579-v · Cited by 51 · see citing works

Mechanism of formation of the A/B cis ring junction of ecdysteroids in Polypodium vulgare

Davies, T. G. E., Lockley, W. J. S., Boid, R., Rees, H. H., & Goodwin, T. W. (1980) · Biochemical Journal · doi:10.1042/bj1900537 · Cited by 38 · see citing works

Formation of the A/B cis ring junction of ecdysteroids in the locust, Schistocerca gregaria

Davies, T. G. E., Dinan, L. N., Lockley, W. J., Rees, H. H., & Goodwin, T. W. (1981) · Biochemical Journal · doi:10.1042/bj1940053 · Cited by 37 · see citing works

Incorporation of acetate and cholesterol into 20-hydroxyecdysone by hairy root clone of Ajuga reptans var. Atropurpurea

Nagakari, M., Kushiro, T., Matsumoto, T., Tanaka, N., Kakinuma, S., & Fujimoto, Y. (1994) · Phytochemistry · doi:10.1016/s0031-9422(00)90461-8 · Cited by 32 · see citing works

The stereochemistry of hydrogen elimination from C-7 during biosynthesis of ecdysones in insects and plants

Cook, I. F., Lloyd-Jones, J. G., Rees, H. H., & Goodwin, T. W. (1973) · Biochemical Journal · doi:10.1042/bj1360135 · Cited by 31 · see citing works

Zur Synthese des Ecdysons. II. Mitteilung über Insektenhormone Synthesen von 2β, 3β‐Dihydroxy‐6‐keto‐A|B‐cis‐Steroiden

Wiechert, R., Kerb, U., Hocks, P., Furlenmeier, A. E., Fürst, A., Langemann, A., & Waldvogel, G. (1966) · Helvetica Chimica Acta · doi:10.1002/hlca.19660490517 · Cited by 28 · see citing works

7-Dehydrosterols in prothoracic glands of the silkworm,Bombyx mori

Sakurai, S., Yonemura, N., Fujimoto, Y., HATA, F., & IKEKAWA, N. (1986) · Cellular and Molecular Life Sciences · doi:10.1007/bf01940720 · Cited by 25 · see citing works

The structures of two ecdysone analogues, cheilanthones A and B, from the fern Cheilanthes tenuifolia

Faux, A.-M., Galbraith, M. N., Horn, D. H. S., Middleton, E. J., & Thomson, J. A. (1970) · Journal of the Chemical Society D Chemical Communications · doi:10.1039/c29700000243 · Cited by 25 · see citing works

7,8-dihydroajugasterone C, norhygrine and other constituents of Nierembergia hippomanica

Pomilio, A. B., González, M. D., & Eceizabarrena, C. C. (1996) · Phytochemistry · doi:10.1016/0031-9422(95)00731-8 · Cited by 23 · see citing works

3β-Hydroxy-5β-cholest-7-en-6-one as an intermediate of 20-hydroxyecdysone biosynthesis in a hairy root culture of Ajuga reptans var. atropurpurea

Nagakari, M., Kushiro, T., Yagi, T., Tanaka, N., Matsumoto, T., Kakinuma, S., & Fujimoto, Y. (1994) · Journal of the Chemical Society Chemical Communications · doi:10.1039/c39940001761 · Cited by 21 · see citing works

3β-Hydroxy-5α-cholestan-6-one: a Possible Precursor of α-Ecdysone Biosynthesis

Sakurai, S., Ikekawa, N., Ohtaki, T., & Chino, H. (1977) · Science · doi:10.1126/science.198.4317.627 · Cited by 17 · see citing works

Early stages of ecdysteroid biosynthesis

Milner, N. P., Nali, M., Gibson, M., & Rees, H. H. (1986) · Insect Biochemistry · doi:10.1016/0020-1790(86)90073-9 · Cited by 17 · see citing works

Early stages of ecdysteroid biosynthesis: The role of 7-dehydrocholesterol

Milner, N. P., Nali, M., Gibson, M., & Rees, H. H. (1986) · Research Explorer (The University of Manchester) · Cited by 16 · see citing works

Biosynthesis of 20-hydroxyecdysone in Ajuga hairy roots: Hydrogen migration from C-6 to C-5 during cis-A/B ring formation

Fujimoto, Y., Kushiro, T., & Nakamura, K. (1997) · Tetrahedron Letters · doi:10.1016/s0040-4039(97)00432-2 · Cited by 16 · see citing works

Biosynthesis of 20-hydroxyecdysone in Ajuga hairy roots

Ohyama, K., Kushiro, T., Nakamura, K., & Fujimoto, Y. (1999) · Bioorganic & Medicinal Chemistry · doi:10.1016/s0968-0896(99)00243-6 · Cited by 13 · see citing works

Steroidal derivatives from the roots of mandevilla pentlandiana

Cabrera, G. M., Palermo, J. A., Seldes, A. M., Gros, E. G., & Oberti, J. C. (1991) · Phytochemistry · doi:10.1016/s0031-9422(00)95209-9 · Cited by 12 · see citing works

A steroidal glycoside from Lepisorus ussuriensis

Choi, Y. H., Kim, J., & Choi, Y. H. (1999) · Phytochemistry · doi:10.1016/s0031-9422(99)00056-4 · Cited by 10 · see citing works

Biosynthesis of phytoecdysone: incorporation of 2β,3β,14α-trihydroxy-5β-cholest-7-en-6-one into β-ecdysone and inokosterone in Achyranthes fauriei

Tomita, Y., & Sakurai, E. (1974) · Journal of the Chemical Society Chemical Communications · doi:10.1039/c39740000434 · Cited by 9 · see citing works

Phytoecdysteroids: Biological aspects

Dinan, L. (2001) · Phytochemistry · doi:10.1016/s0031-9422(01)00078-4 · Cited by 392 · see citing works

The Phytochemical, Biological, and Medicinal Attributes of Phytoecdysteroids: An Updated Review

Das, N., Mishra, S. K., Bishayee, A., Ali, E. S., & Bishayee, A. (2021) · Acta Pharmaceutica Sinica B · doi:10.1016/j.apsb.2020.10.012 · Cited by 115 · see citing works

Phytoecdysteroids: Diversity, biosynthesis and distribution

Dinan, L., Harmatha, J., Volodin, V., & Lafont, R. (2009) · Ecdysone: Structures and Functions · doi:10.1007/978-1-4020-9112-4_1 · Cited by 77 · see citing works

Phytoecdysteroids: Distribution, Structural Diversity, Biosynthesis, Activity, and Crosstalk with Phytohormones

Arif, Y., Singh, P., Bajguz, A., & Hayat, S. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms23158664 · Cited by 50 · see citing works

Biosynthesis of sterols and ecdysteroids in <i>Ajuga</i> hairy roots

Fujimoto, Y., Ohyama, K., Nomura, K., Hyodo, R., Takahashi, K., Yamada, J., & Morisaki, M. (2000) · Lipids · doi:10.1007/s11745-000-0524-z · Cited by 45 · see citing works

Recent advances in cholesterol chemistry

Morzycki, J. W. (2014) · Steroids · doi:10.1016/j.steroids.2014.02.001 · Cited by 39 · see citing works

Functional characterization of CYP71D443, a cytochrome P450 catalyzing C-22 hydroxylation in the 20-hydroxyecdysone biosynthesis of Ajuga hairy roots

Tsukagoshi, Y., Ohyama, K., Seki, H., Akashi, T., Muranaka, T., Suzuki, H., & Fujimoto, Y. (2016) · Phytochemistry · doi:10.1016/j.phytochem.2016.03.010 · Cited by 27 · see citing works

Genetically transformed root cultures — generation, properties and application in plant sciences

Kuzovkina, I. N., & Schneider, B. (2006) · Springer eBooks · doi:10.1007/3-540-27998-9_13 · Cited by 26 · see citing works

Ajuga Δ24-Sterol Reductase Catalyzes the Direct Reductive Conversion of 24-Methylenecholesterol to Campesterol

Tsukagoshi, Y., Suzuki, H., Seki, H., Muranaka, T., Ohyama, K., & Fujimoto, Y. (2016) · Journal of Biological Chemistry · doi:10.1074/jbc.m115.703470 · Cited by 24 · see citing works

Distribution and Biosynthesis of 20-Hydroxyecdysone in Plants ofAchyranthes japonicaNakai

Boo, K.-H., Lee, D., Jeon, G. L., Ko, S. H., Cho, S. K., Kim, J. H., Park, S., Hong, Q. C., Lee, S., Lee, D., et al. (2010) · Bioscience Biotechnology and Biochemistry · doi:10.1271/bbb.100410 · Cited by 21 · see citing works

Sustainable Production of Ajuga Bioactive Metabolites Using Cell Culture Technologies: A Review

Popova, E., Титова, М. В., Тыныкулов, М., Zakirova, R. P., Куличенко, И. Е., Prudnikova, O. N., & Nosov, A. M. (2023) · Nutrients · doi:10.3390/nu15051246 · Cited by 20 · see citing works

Biosynthesis of 20-hydroxyecdysone in plants: 3β-Hydroxy-5β-cholestan-6-one as an intermediate immediately after cholesterol in Ajuga hairy roots

Fujimoto, Y., Maeda, I., Ohyama, K., Hikiba, J., & Kataoka, H. (2015) · Phytochemistry · doi:10.1016/j.phytochem.2014.12.019 · Cited by 12 · see citing works

Ecdysone 20-Monooxygenase Activity of Cytochrome P450 in Ajuga reptans L. Plants and Cell Culture

Alekseeva, L. I. (2004) · Applied Biochemistry and Microbiology · doi:10.1023/b:abim.0000018915.75810.a7 · Cited by 11 · see citing works

20-hydroxyecdysone accumulation and regulation in Ajuga lobata D. Don suspension culture

Qian, J., Yang, Y., Li, X., & Chi, D. (2015) · Bioscience Biotechnology and Biochemistry · doi:10.1080/09168451.2015.1116921 · Cited by 8 · see citing works

Transcriptome analysis of abscisic acid induced 20E regulation in suspension Ajuga lobata cells

Wang, Y., Yang, Y., & Chi, D. (2018) · 3 Biotech · doi:10.1007/s13205-018-1352-6 · Cited by 6 · see citing works

Advances in Genetic Engineering of Ajuga Species

Kayani, W. K., Hasan, H., & Mirza, B. (2018) · Springer eBooks · doi:10.1007/978-981-13-0535-1_27 · Cited by 5 · see citing works

Identification of oxidoreductase genes for phytoecdysteroids biosynthesis in spinach through comparative transcriptomics

Kim, J., Yang, X. U., Riu, K.-Z., Kim, J. H., Hyun, C., & Boo, K.-H. (2025) · Plant Physiology and Biochemistry · doi:10.1016/j.plaphy.2025.110216 · Cited by 4 · see citing works

Review of culture media and serum substitutes containing phytoecdysteroids for application in cultured meat technology

Park, J., Lee, D. Y., Mariano, E., Choi, Y., Han, D., Kim, J. S., Park, J. W. P. J. W., Namkung, S., Lee, S. Y., Lee, H. W., et al. (2026) · Food Science of Animal Resources · doi:10.1007/s44463-025-00003-2

Convergent biosynthesis of insect molting hormones in plantsPreprint

Park, J. J., Orozco, J. A., Tiong, G. J. L., Kim, C. Y., & Nett, R. S. (2026) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.64898/2026.08.07.743582

Biosynthesis of 20-hydroxyecdysone in plants: 3β-hydroxy-5β-cholestan-6-one as an intermediate immediately after cholesterol in Ajuga hairy roots

藤本, 善., & FUJIMOTO, Y. (2014) · Institutional Repositories DataBase (IRDB)

3β-Hydroxy-5β-cholest-7-en-6-one as an intermediate of 20-hydroxyecdysone biosynthesis in a hairy root culture of Ajuga reptans var. atropurpurea

Nagakari, M., Kushiro, T., Yagi, T., Tanaka, N., Matsumoto, T., Kakinuma, S., & Fujimoto, Y. (1994) · Journal of the Chemical Society Chemical Communications · doi:10.1039/c39940001761 · Cited by 21 · see citing works

Biosynthesis of 20-hydroxyecdysone in Ajuga hairy roots: Hydrogen migration from C-6 to C-5 during cis-A/B ring formation

Fujimoto, Y., Kushiro, T., & Nakamura, K. (1997) · Tetrahedron Letters · doi:10.1016/s0040-4039(97)00432-2 · Cited by 16 · see citing works

3β-Hydroxy-5β-cholest-7-en-6-one as an Intermediate of 20-Hydroxyecdysone Biosynthesis in Hairy Root Culture of Ajuga reptans var. atropurpurea

柿沼勝己, & KAKINUMA, K. (2006) · Tokyo Tech Research Repository (Tokyo Institute of Technology)

Biosynthesis of sterols and ecdysteroids in <i>Ajuga</i> hairy roots

Fujimoto, Y., Ohyama, K., Nomura, K., Hyodo, R., Takahashi, K., Yamada, J., & Morisaki, M. (2000) · Lipids · doi:10.1007/s11745-000-0524-z · Cited by 45 · see citing works